Cyanine Dye Oligo Labeling
Custom DNA Synthesis--"FASTEST services in the industry"
DNA Modifications
Cy® Fluorophores
Cy® dyes exhibit excellent spectroscopic performance with high quantum yields, high
extinction coefficient, and good stability of fluorescence signal against changes
in pH in various buffer conditions. In addition to labeling oligonucleotides chemical
during synthesis, we can also attached Cy3, Cy3.5, Cy5, Cy5.5 and Cy7 fluorescent
DNA probes by chemically by preparing 5'-amino-end-labeled oligonucleotide probes
on a DNA synthesizer. The amino-group containing probes are then labeled with a
fluorescent tag containing an isothiocyanate group or a succinimidyl active ester.
Contact your Account Representative for pricing on Cy dye labeled oligonucleotides.
For additional information, please Contact us.
Price/labeling, purification and synthesis is an additional charge
| Dyes |
Scales |
Scales |
| 5'
|
Int
|
3'
|
| Cy3Tm
|
250 nmol
|
$235
|
$295*
|
$235 |
| 1.0 µmol
|
$375 |
$450*
|
$375 |
| Cy3.5Tm
|
250 nmol
|
$395 |
$450*
|
$395*
|
| 1.0 µmol
|
$350
|
$450*
|
$350*
|
| Cy5Tm
|
250 nmol
|
$235
|
$295*
|
$235
|
| 1.0 µmol
|
$375
|
$450*
|
$375
|
| Cy5.5Tm |
250 nmol |
$265 |
$395* |
$265* |
| 1.0 µmol |
$485 |
$496* |
$485* |
* Product Note - This compound requires amino- or thiol- linker with a 6-Carbon
spacer arm or by substituting any thymidine with 5-C6-Amino-2'-deoxythymidine for
internal labeling, a double HPLC purification is required.
Purification
A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Quality Controls
260 In addition, we perform QC methods tailored to specific modifications,
such as OD ratio measurement where appropriate.
For additional information, please Contact us.
The most trusted name in Quality Custom Synthesis