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Sirna Oligos Oligonucleotides Enzymatic Degradation

How to handle and store my siRNA oligo?

Storage:

  1. siRNA oligos are shipped as dry pellets at room temperature, Under these condition, they are stable for at least four weeks.
  2. Upon receipt, siRNA reagents should be stored at -20OC to -80OC.  Under these conditions, they are stable for at least one year.
  3. siRNA should be resuspended in RNase-free solutions.  RNase-free water (for short-term storage) is appropriate for resuspension of concentrated stocks (i.e. 20-100 µM).  Alternatively, an RNase-free buffer (pH7.3-7.6) maybe used such as PBS.

Handling Precautions:

Oligonucleotides are susceptible to enzymatic degradation by nucleases and to chemical degradation by extreme pH and temperature.  We recommend wearing gloves and maintaining nuclease-free conditions when handling the oligonucleotides.