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Bio-Synthesis Inc. Introduces "BNA-NC" - A Third Generation Multi Functional Bridged Nucleic Acid
November 07, 2012
Bio-Synthesis, Inc. has acquired a license from BNA Inc. of Osaka, Japan for the manufacturing and distribution of BNA-NC, a third generation of BNA oligonucleotides.
Bio-Synthesis is proud to announce that it has acquired a license from BNA Inc. of Osaka, Japan for the manufacturing and distribution of BNA-NC, a third generation of BNA oligonucleotides. This agreement is valid worldwide and is meant for research use only at this time.
In 1997, the first generation BNA (2’-O ,4’-C-methylene-bridged nucleic acid, 2’,4’-BNA, also known as LNA) was developed by Prof. Takeshi Imanishi of Osaka University (now, Emeritus Professor and CEO of BNA Inc.) and co-workers. This was followed by development of second generation BNAs such as 2’,4’-BNA-COC, 5’-amino-2’,4’-BNA.
The third generation of 2’,4’-BNA-NC contains a 6-membered ring with a N-O bond between the 2’-hydroxyl and the 4’-carbon of the ribose moiety. It exhibits very strong binding affinity to complementary single stranded RNA, DNA, and double stranded DNA with a highly sequence-selective mode. In particular, the binding ability to complementary RNA is significantly high. BNA-NC also possesses great stability due to its nuclease resistant properties.

Dr. Miguel Castro commented: "In the past two decades we have seen the development of chemically modified oligonucleotides with enhanced binding properties to DNA and RNA. Most of these oligonucleotides also have increased affinity to complementary DNA. Some analogs such as Peptide Nucleic Acids (PNA) are fairly stable. However, they also have several disadvantages including a tendency to aggregate, limitation in size due to solubility issues and difficulty in integrating into chimeras of mixed oligos. The first generation BNA (2’,4’-BNA/LNA) has good RNA binding affinity and fair stability to exonucleases. Third generation BNA (BNA-NC) improves on that by exhibiting superior binding affinity towards single stranded RNA, a better binding affinity to double stranded DNA and excellent enzymatic stability to exonucleases, thereby making it a great choice for many biological applications.
"We aim to expand our product offering with oligonucleotides which are sufficiently stable in physiological conditions and bind well to RNA complements. Variation of N-substitution at the 6-membered ring will result in additional advantages for biological applications. We are very excited to introduce BNA-NC oligonucleotides to the biomedical research community. These oligonucleotides will have a great impact in antisense, siRNA, SNP detection, FISH as well as a variety of diagnostic applications. BNA-NC can be used both as a primer and as a probe in a myriad of PCR and RT-PCR applications as well as applications in Molecular Biology research and commercial areas." 

Contact us to learn more about Bridged Nucleic Acids (BNAs) services.