Photocleavable Linker (PC Linker) often used in the design of multi-functional single-stranded nucleotide conjugates for the selection of novel DNA or RNA-based catalysts in vitro. Photocleavable PC linker modified oligonucleotide are demonstrated in Bruker Daltonik's genoSNIP, a MALDI-TOF MS based assay system for SNP detection.
Photocleavable PC linker contained a non-nucleosidic group that can be used to link two nucleotide sequences through a short, UV photo-cleavable C3 spacer arm. Upon irradiation by UV light, photo-cleavage released oligonucleotide contain a 5-phosphorylated group with greater hybridization affinity for a complementary DNA strand than PC spacer.
Photocleavable PC linker can be incorporate at any position within an oligonucleotide. Contact Bio-Synthesis for service details.
Cleavage occurs by irradiation with near-UV light (300-350 nm, complete cleavage occurs within 5 minutes. Try using a Black Ray XX-15 UV lamp (Ultraviolet Products Inc., San Gabriel, CA) at a distance of 15 cm (emission peak 365 nm, 300 nm cut-off, 1.1 mW intensity at~31 cm).
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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