Thiazole orange is an asymmetric cyanine dye with a fluorescent assymmetic cyanine exited at 514 nm and an emission peak at 533 nm when an oligonucleotide labeled with thiazole orange is hybridized to its complementary sequence, the thiazole orange acts as an intercalator. In addition to providing enhanced thermal stability, the dye adopts a mostly planar configuration resulting in as high as 34 fold light up capability which significantly enhanced fluorescence.

  • Up to 3.9 to 8.6 fluorescent enhancement during hybridization
  • Ehanced duplex stability which allow short probe design
  • High sensitve, multi-color detection of DNA and RNA

Product Information


Product Name:

Thiazole Orange NHS Ester



Delivery Format:


Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.


1. F.H. Martin, M.M. Castro, F. Aboul-ela, and I. Tinoco, Jr., Nucleic Acids Res, 1985, 13, 8927-38.
2. E. Ohtsuka, S. Matsuki, M. Ikehara, Y. Takahashi, and K. Matsubara, J Biol Chem, 1985, 260, 2605-8.
3. R.V. Patil, and E.E. Dekker, Nucleic Acids Res, 1990, 18, 3080