Benefit of Using PlexorTM Primers:
- Simple primer design
- Does not require a separate probe
- Powerful multiplexing capability
Choosing Scorpions Probes for:
- Gene Expression
- SNP Genotyping
How does PlexorTM Primers Work?
PlexorTM Primer technology is a unique real-time PCR chemistry based on the specific interaction between two modified nucleotides: iso-dC and iso-dG. These two modified nucleotides only pair with each other when forming double-stranded DNA. Plexor assays incorporate iso-dC residue and a fluorescent label on the 5' end of the forward primer while the reaction mix includes DABCYL-labeled iso-dG, along with unlabeled reverse primers.
The first step involves heating to denature the double-stranded target DNA into single-stranded DNA. During the second step, the forward primer containing the 5' modified iso-dC and fluorophore anneals to the target DNA and is extended by polymerase. In the next cycle, the resulting double-stranded DNA is melted and the unlabeled reverse primer anneals and is extended by polymerase. When the polymerase encounters the 5' iso-dC, a modified iso-dGTP is added instead of a standard guanine. The binding of fluorophore linked iso-dC and quencher linked iso-dG is brought into close proximity, allowing the fluorescence to be quenched, resulting in a reduction in fluorescent signal.
Plexor Primer for Gene Expression and Genotyping
If you can't find the dye and quencher combination listed below, please contact us
PlexorTM Unlabeled Companion Primers
PlexorTM Primers Human Control Gene Primer Sets
|
Deliver Yields (HPLC purified)
|
Item No
|
| PlexorTM Human GAPHD Primer Set, 5' Texas Red |
|
$108.00 |
| PlexorTM Human GAPDH Primer Set, 5' HEX |
|
$108.00 |
| PlexorTM Human GAPDH Primer Set, 5' CAL Fluor Organge 560 |
|
$108.00 |
| PlexorTM Human GAPDH Primer Set, 5' CAL Fluor Organge Red 610 |
|
$108.00 |
| PlexorTM Human GAPDH Primer Set, 5' CAL Fluor Quasar 670 |
|
$108.00 |
| PlexorTM ß-Actin Primer Set, 5' Texas Red |
|
$108.00 |
| PlexorTMß-Actin Primer Set, 5' HEX |
|
$108.00 |
| Pre-mixed PlexorTM Control Gene Primer Set contains 0.5 nmoles of the PlexorTM Primer and an unlabeled companion reverse primer. |
Purification
In order to ensure that there is no background fluorescence, 100% of the dual labeled probes are purified by single or dual rp-HPLC and ie-HPLC as a standard protocol for all dual–labeled probes which give 90-97% purity. Depending on the type of the probes, one or two purification may performed to ensure the highest purity level
Quality Control
All dual labeld probes are quality checked by MALDI-TOP Mass spectrometry , analytical HPLC and analyze by Fluorometric Scan (ABS/EM) . In addition, the signal-to-background ratio is measured against a target sequence for all molecular beacons.
Delivery times
3-5 Working days
Packaging
Lyophilised
Shipping conditions
Room temperature
Storage conditions
-20 oC to -70 oC
Oligonucleotides are stable in solution at 4oC for up to 2 weeks. Properly reconstituted material stored at -20oC should be stable for at least 6 months. Lyophilized DNA (when kept at -20oC) in a nuclease-free environment should be stable for year
Specifications
Quality control:
Every oligo is quality checked by MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE).
Purification
Fully deprotected and desalted. Purified by PAGE or RP-HPLC
Length
|
Length |
| LightCycler probes |
15 to 40 mers (optimal length: 20 to 30 mers) |
| Dual-labeled fluorogenic probes: |
15 to 40 mers |
| Molecular Beacons |
15 to 40 mers |
| Scorpions probes |
30 to 60 mers (uni-probe) |
| 15 to 45 mers (bi-probe) |
| Plexor |
up to 30 mers |
Backbone
Phosphodiester bond
Format
Delivered as dried-down product in opaque tubes
Turn-around time:
|
Turn-around time |
| LightCycler probes |
4 to 5 working days |
| Dual-labeled fluorogenic probes: |
5 to 6 working days |
| Molecular Beacons |
5 to 6 working days |
| Scorpions probes |
7 to 10 working days |
Turn-around time is dependent upon successful QC validation and does not include shipping time.
Storage and stability:
See information on our oligos: storage recommendations
Shipment:
Shipped by mail or express delivery, dry, in individual, opaque tubes
Technical Documentation:
Oligonucleotides are delivered with an OligonucleotideTechnical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, length, extinction coefficient and purification data.
Additional Services:
- Probe design services
- High-throughput screening formats (microplates, etc)
- Aliquoting
Additional services may increase turn-around time
Pricing:
Please contact your local Bio-Synthesis representative
Ordering:
On-line (contact us), by email (info@biosyn.com) or fax