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Bio-synthesis is offering PlexorTM primers based on Promega’s PlexorTM technology for real-time qPCR to multiplex assays. This technology is based on highly specific interaction between two novel nucleotides, isoguanine (iso-dG) and 5'-methylisocytosine (iso-dC) which form a unique base pair when incorporated in double-stranded DNA and pair only with each other. As amplification progresses, the binding of iso-dC (linked to a flourophore) and iso-dG (linked to a quencher) bring the flourophore and quencher into close proximity, allowing the flourescence to be quenched, reducing the fluorecence at the level proportional to the amount of PCR product generated. The quenching effect is reversible, so melt curve analysis can be performed using this technology. Plexor primers commonly used for Gene Expression and SNP Genotyping
All Bio-Synthesis's Plexor Primers are available at a fixed price per oligonucleotide, within the following parameters:
Please contact technical support for assistance with design of Plexor Primers
PlexorTM Primer technology is a unique real-time PCR chemistry based on the specific interaction between two modified nucleotides, iso-dC and iso-dG. These two modified nucleotides only pair with each other when forming double-stranded DNA. Plexor assays incorporate iso-dC residue and a fluorescent label on the 5' end of the forward primer while DABCYL-labeled iso-dG are included in the reaction mix along with unlabeled reverse primers.
The first step involves heating to denature the double-stranded target DNA into single-stranded DNA. During the second step, the forward primer containing the 5' modified iso-dC and fluorophore anneals to the target DNA and is extended by polymerase. In the next cycle, the resulting double stranded DNA is melted and the unlabled reverse primer annleas and is extended by polymerase. When the polymerase encoutners the 5' iso-dC, a modified iso-dGTP is added instead of a standard guanine. The binding of fluorophore linked iso-dC and quencher linked iso-dG are brought into close proximity, allowing the fluorescence to be quenched, resulting in a reduction in fluorescent singnal.
If you can't find the dye and quencher combination listed below, please contact us
Forward PlexorTM Primer
Deliver Yields (dual HPLC purified)
No. of nmole
No. of µg
Reversed PlexorTM Primer
Deliver Yields (HPLC purified)
In order to ensure that there is no background fluorescence, 100% of the dual labeled probes are purified by single or dual rp-HPLC and ie-HPLC as a standard protocol for all dual–labeled probes which give 90-97% purity. Depending on the type of the probes, one or two purification may performed to ensure the highest purity level
All dual labeld probes are quality checked by MALDI-TOP Mass spectrometry , analytical HPLC and analyze by Fluorometric Scan (ABS/EM) . In addition, the signal-to-background ratio is measured against a target sequence for all molecular beacons.
3-5 Working days
-20 oC to -70 oC
Oligonucleotides are stable in solution at 4oC for up to 2 weeks. Properly reconstituted material stored at -20oC should be stable for at least 6 months. Lyophilized DNA (when kept at -20oC) in a nuclease-free environment should be stable for year
Every oligo is quality checked by MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE).
Fully deprotected and desalted. Purified by PAGE or RP-HPLC
Delivered as dried-down product in opaque tubes
Turn-around time is dependent upon successful QC validation and does not include shipping time.
See information on our oligos: storage recommendations
Shipped by mail or express delivery, dry, in individual, opaque tubes
Oligonucleotides are delivered with an OligonucleotideTechnical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, length, extinction coefficient and purification data.
Additional services may increase turn-around time
Please contact your local Bio-Synthesis representative
On-line (contact us), by email (firstname.lastname@example.org) or fax