Enhanced Diagnostic Tools
Several techniques are available at Bio-Synthesis to evaluate the quantity, quality,
and function of the antibodies produced. BSI's Protein Analytical Methods can provide you with important and detailed information about polyclonal and monoclonal antibodies and help you throughout the development phase.
Bio-Synthesis can provide you with detailed characterization of your antibody including:
To obtain further information, please contact us.
The multiplex Luminex® assay format differs from conventional ELISA in one significant way - the multiplex capture antibody is attached to a polystyrene bead whereas the ELISA capture antibody is attached to the microplate well.
Luminex assays are based on xMAP technology (multi-analyte profiling beads) enabling the detection and quantitation of multiple RNA or protein targets simultaneously. The xMAP system combines a flow cytometer, fluorescent-dyed microspheres (beads), lasers and digital signal processing to effectively allow multiplexing of up to 100 unique assays within a single sample.
Bio-Synthesis can guide you through all the steps starting from your needs finishing with your multiplex immunoassay.
See details on Luminex Assay Development and Services.
ELISAs (Enzyme Linked Immunosorbent Assays) are used to measure the specific binding between an antigen and antibody using a secondary enzyme labeled antibody and substrate system. This is a rapid and sensitive method for evaluating large numbers of monoclonal or polyclonal antibodies at low cost. The scientists at BSI have years of experience developing ELISAs for our clients and for our own in-house antibodies and we are pleased to share that experience with you. Once an ELISA has been developed for you, we will transfer the ELISA protocol to you and, if you wish, apply that ELISA to testing of your antibody samples.
This technique permits identification of proteins by their molecular weight and their immunoreactivity or the control of the immunoreactivity of serum on the denaturated protein of interest. The sample is prepared and loaded into a pre-cast 10% to 12% Tris-HCl denaturing gel. The standards are included with molecular weights ranging from 6.5kD to 200kD. Two bands are observed after staining in Coomassie Blue rep-resenting the heavy chain (55kD) and the light chain (25kD) of the antibody. Each client is provided with a photo of the gel as an indicator of purity.
The isotype of murine antibodies (either in supernatants or ascites) can be determined using murine isotyping kits or strips.
For additional antibody characterization services, visit our proteomic analytical services.