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Bio-Synthesis's BNA Genotyping Probes are dual-labeled DNA probes designed fro us in 5' nuclease assays. BNA enhanced oligonucleotide are ideal when studying short or very similar sequences. The high affinity of an BNA oligo to its complementary sequence results in improved specificity and sensitivity compared to traditional DNA or RNA oligonucletoides, it is an idea solution for SNP genotyping assays.
Bridged Nucleic Acids (BNAs) bases can significantly increase Tm of an oligonucleotide when incorporated into dual-labeled probe. BNA probes can be designed with shorter lengths than standard dual labeled probes. Shorter probe design permits better quenching, increased signal to noise ratio and are there more, more sensitive. These probes offer an improved ability to discriminate mutation or closely related sequences. BNA enhanced dual labeled probe can have a Tm > 15oC between perfect-match and mismatch hybridization. Ability to modulate Tm greatly increase accuracy of allele determination in real-time PCR or other detection system which require differential hybridization to discriminate closely related sequences.
When designing allele-specific primers or probes for SNP detection, vary the length and BNA™ positioning to obtain comparable melting temperatures ( Tm) for the alleles, while keeping the difference in melting temperatures ( Δ Tm) between the perfect match and mismatch binding as high as possible.
BNATM-enhanced clamps are oligonucleotides that compete with probes and primers for binding. They are very similar to the primers or probes they compete with, but are designed to perfectly match undesired PCR products or templates that should not be amplified.
Select your own BNA SNP probe using service package below or design your own using different dyes and quencher combination. Our BNA genotyping probes includes:
Every oligo is quality checked by MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE).
Fully deprotected and desalted. Purified by PAGE or RP-HPLC
Delivered as dried-down product in opaque tubes
Turn-around time is dependent upon successful QC validation and does not include shipping time.
See information on our oligos: storage recommendations
Shipped by mail or express delivery, dry, in individual, opaque tubes
Oligonucleotides are delivered with an OligonucleotideTechnical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, length, extinction coefficient and purification data.
Additional services may increase turn-around time
Please contact your local Bio-Synthesis representative
On-line (contact us), by email (email@example.com) or fax