Live Chat Support Software
800.227.0627

Peptide Library Services

Peptide Library Services

Peptide Library Synthesis

Peptide Library is a large collection of synthetic peptides with a systematic combination of amino acids. These synthetic peptide library screening tools are used in epitope peptide mapping, peptide library screening for drug target validation and vaccine development. A typical peptide library contains a maximum of 25 amino acids. However, we can synthesize peptides of up to 30 amino acids in length in order to ensure that potential proteotypic peptides needed to be screened for an SRM assay are covered.

Peptide libraries are synthesized on our proprietary high throughput combinatorial peptide synthesis platform. Final unbound peptides are carefully placed in individual vials to avoid cross contamination or alternatively, packaged in a 96 well tube plate.

Our synthetic peptide libraries offer a flexible selection of peptide purity levels ranging from crude to 98% purity. Each random peptide in the library undergoes rigorous quality control to prevent any cross contaminants before delivery. All purified peptides are delivered with a QC reports of RP-HPLC, MS, and COA to ensure the highest quality.

Bio-Synthesis has created seven powerful Peptide Library Design Tools which are available online for generating peptide libraries. These tools enable you to design a large selection of random peptide libraries to screen for highly active compounds such as antigenic peptides, receptor ligands, antimicrobial compounds and enzyme inhibitors.

For additional information, please contact us or send an email to info@biosyn.com


What is a Peptide Library?

With the advent of combinatorial chemistry and the establishment of automated solid phase synthesis technologies, it became possible to synthesize large numbers of peptides in large quantities. These libraries are useful for the development of therapeutic drugs as well as for the design of peptide libraries to allow for screening of their activity using various combinations of screening technologies such as epitope mapping among others.

By combining different resins with linker molecules and coupling methods, a wide range of libraries can now be synthesized. After synthesis, libraries can be cleaved from the resin to yield peptide libraries in solution that can be used in various formats or for spotting onto supports such as membranes made of cellulose, pvdf or coated glass slides. Furthermore, peptide libraries can also be left on the resin or beads after synthesis. The use of a defined linker has proven to be beneficial for libraries on solid supports.

Applications of Peptide Library

Solid phase peptide synthesis as well as other methods that allow the creation of peptide libraries have been successfully used to prepare libraries for the following applications:

  • Antibody epitope mapping and validation to determine the specificity of antibodies
  • Identification of bioactive peptides
  • Development of biological assays
  • T-cell epitope mapping
  • Vaccine efficacy testing
  • Screening for ligand-binding activities
  • Screening for antimicrobial peptide activities
  • Screening for peptide-protein interactions
  • Libraries for drug discovery
  • Libraries for affinity optimization
  • Peptide libraries for LC-MS/MS method development and validation
  • Libraries for receptor-ligand studies and cellular assays
  • Libraries with constrained peptides
  • Libraries containing peptides with modifications such as histone peptides
  • Screening for MHC class I and class II peptides
  • Many others

Type of Peptide Libraries

  • On-resin or off-resin libraries
  • Libraries containing scrambled amino acid residue peptides
  • Peptide libraries containing specific linker molecules
  • Dye-labeled peptide libraries
  • Libraries with special modifications
  • Polyepitope peptide library for vaccine development
  • Other combinatorial libraries

Literature related to this subject indicates that synthetic peptide libraries can be used to identify biologically active peptides. For example, Smith et al. in 1994 used a random synthetic library of nonamer peptides to identify peptide motifs that bind to human class I major histocompatibility complex molecules HLA-A2 and B7. The identified allele-specific motifs for class I molecules were sequenced to identify the peptides that bound to the class I protein. These methods have the potential to detect peptides which may be valuable in predicting and engineering immunomodulatory T cell epitopes.

Peptide Pools contain a selection of peptides similar to a peptide library and are ideal for efficient in vitro stimulation of antigen-specific CD4+ and CD8+ T cells. If the pool contains 15-mer peptides with an 11 amino acid overlap, complete sequence of the respective antigen can be covered. This approach has been shown to be very effective in stimulating CD4+ as well as CD8+ T cells in various applications.

Peptide Arrays are similar to solid phase peptide libraries. Arrays of peptides may serve as valuable tools for identifying biologically active motifs as well as for profiling cellular activities, among other biological applications. Several methods for the preparation of peptide arrays have been reported. Arrays can be prepared by in situ peptide synthesis or by immobilization of functionalized peptides onto various supports.

Contact us for pre-made pools and arrays for your specific applications.

Key Features

  • Optimized Chemistry: Qquality, purity and batch to batch consistency
  • No Cross Contamination: Directly lyophilized in 96 tube-plates or in individual vials
  • Flexible Options: Crude to 95% pure from 1 to 100 mgs scales
  • Comprehensive Modifications: Biotin, fluorescence, unnatural A.A.
  • Stringent Quality Control: COA, MS and HPLC validation data for each
  • Fast Turnaround Time: 2-3 week delivery
  • Vaccine Development: Polyepitope peptide library for vaccine development
  • Made in USA: No third pary outsourcing

Peptide Library Synthesis Services

Bio-Synthesis has developed several peptide library service packages with different purity levels to accommodate multiple applications. On-demand requests such as large amount, modifications or additional QC also available upon request.

Peptide library PL1000-48-1 is designed for applications in T-cell epitope discovery, biological screening or identification of proteotypic peptides which require large number of peptides quickly and affordably.

This synthetic peptide library is composed of 48 crude peptides, 7-15 amino acid in length, 2 mgs each with 5% random QC per each peptide library set. Longer peptides of higher purity or larger quantity (10mg, 20mg, 50mg, or 100mg) are also available upon request

Purity: Crude
Qty: 2-3 mgs (large quantity available)
Peptide Length: 7-15 aa
Counter-ion: TFA
QC/QA: 5% MALDI MS analysis of peptides
Formulation: lyophilized in tube or tube-plates
Delivery Time: 1-2 weeks
Price/peptide: Inquire
Minimum order: 48 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL1000-48-2 is designed for applications in T-cell assays when fully analysized peptides is required to prevent cross contamination and failure sequences which may result with false positive T-cell response or toxic inhibition.

This synthetic peptide library is composed of 48 crude peptides, 7-15 amino acid in length, 2 mgs each with 100% MALDI mass spectrometry analysis of every peptides. Longer peptides of higher purity or larger quantity (10mg, 20mg, 50mg, or 100mg) are also available upon request.

Purity: Crude
Qty: 2-3 mgs (large quantity available)
Peptide Length: 7-15 aa
Counter-ion: TFA
QC/QA: 100% MALDI MS analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 1-2 weeks
Price/peptide: Inquire
Minimum order: 48 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL1000-48-3 is designed for applications in T-cell assays when fully analysized peptides is required to prevent cross contamination and failure sequences which may result with false positive T-cell response or toxic inhibition.

This synthetic peptide library is composed of 48 crude peptides, 7-15 amino acid in length, 2 mgs each with 100% MALDI mass spectrometry and HPLC analysis of every peptides. Longer peptides of higher purity or larger quantity (10mg, 20mg, 50mg, or 100mg) are also available upon request.

Purity: Crude
Qty: 2-3 mgs (large quantity available)
Peptide Length: 7-15 aa
Counter-ion: TFA
QC/QA: 100% MALDI MS and HPLC analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 1-2 weeks
Price/peptide: Inquire
Minimum order: 48 peptides

*For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL1000-96-1 is designed for applications in T-cell epitope discovery, biological screening or identification of proteotypic peptides which require large number of peptides quickly and affordably.

This synthetic peptide library is composed of 48 crude peptides, 7-15 amino acid in length, 2 mgs each with 5% random QC per each peptide library set. Longer peptides of higher purity or larger quantity (10mg, 20mg, 50mg, or 100mg) are also available upon request.

Purity: Crude
Qty: 2-3 mgs (large quantity available)
Peptide Length: 7-15 aa
Counter-ion: TFA
QC/QA: 5% MALDI MS analysis of peptides
Formulation: lyophilized in tube or tube-plates
Delivery Time: 1-2 weeks
Price/peptide: Inquire
Minimum order: 96 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL1000-48-2 is designed for applications in T-cell assays when fully analysized peptides is required to prevent cross contamination and failure sequences which may result with false positive T-cell response or toxic inhibition.

This synthetic peptide library is composed of 96 crude peptides, 7-15 amino acid in length, 2 mgs each with 100% MALDI mass spectrometry analysis of every peptides. Longer peptides of higher purity or larger quantity (10mg, 20mg, 50mg, or 100mg) are also available upon request.

Purity: Crude
Qty: 2-3 mgs (large quantity available)
Peptide Length: 7-15 aa
Counter-ion: TFA
QC/QA: 100% MALDI MS analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 1-2 weeks
Price/peptide: Inquire
Minimum order: 96 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL1000-96-2 is designed for applications in T-cell assays when fully analysized peptides is required to prevent cross contamination and failure sequences which may result with false positive T-cell response or toxic inhibition.

This synthetic peptide library is composed of 96 crude peptides, 7-15 amino acid in length, 2 mgs each with 100% MALDI mass spectrometry and HPLC analysis of every peptides. Longer peptides of higher purity or larger quantity (10mg, 20mg, 50mg, or 100mg) are also available upon request.

Purity: Crude
Qty: 2-3 mgs (large quantity available)
Peptide Length: 7-15 aa
Counter-ion: TFA
QC/QA: 100% MALDI MS and HPLC analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 1-2 weeks
Price/peptide: Inquire
Minimum order: 96 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL2000-48-70 has similar and specified purity of >70% for all peptides in the pool. It is ideal for immune monitoring applications. These are fully analyzed peptides by MALDI-MS and analystical HPLC in quantity of 1-5 mgs for peptides with 7-18 amino acids in length. Longer peptide size, higher purity or large quantity(10mg, 20mg, 50mg, 100mg) also available upon request.

Purity: 70% pure
Qty: 1-5 mgs (large quantity available)
Peptide Length: 7-18 aa
Counter-ion: TFA
QC/QA:100% MALDI MS and HPLC analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 2-3 weeks
Price/peptide: Inquire
Minimum order: 48 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL2000-96-70 has similar and specified purity of >70% for all peptides in the pool. It is ideal for immune monitoring applications. These are fully analyzed peptides by MALDI-MS and analystical HPLC in quantity of 1-5 mgs for peptides with 7-18 amino acids in length. Longer peptide size, higher purity or large quantity(10mg, 20mg, 50mg, 100mg) also available upon request.

Purity: 70% pure
Qty: 1-5 mgs (large quantity available)
Peptide Length: 7-18 aa
Counter-ion: TFA
QC/QA:100% MALDI MS and HPLC analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 2-3 weeks
Price/peptide: Inquire
Minimum order: 96 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL3000-48-80 has a specified purity of >80% for all peptides in the pool. It is designed as a monitoring tool for vaccination screening. These peptides are fully analyzed by MALDI-MS and analytical HPLC in quantities of 1-5 mgs per peptide with 7-18 amino acids in length. Longer peptide sizes, higher purity, large quantity (10mg, 20mg, 50mg, 100mg) or extended analytical services are also available upon request.

Purity: 80% pure
Qty: 1-5 mgs (large quantity available)
Peptide Length: 7-18 aa
Counter-ion: TFA
QC/QA:100% MALDI MS and HPLC analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 2-3 weeks
Price/peptide: Inquire
Minimum order: 48 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL3000-96-80 has a specified purity of >80% for all peptides in the pool. It is designed as a monitoring tool for vaccination screening. These peptides are fully analyzed by MALDI-MS and analytical HPLC in quantities of 1-5 mgs per peptide with 7-18 amino acids in length. Longer peptide sizes, higher purity, large quantity (10mg, 20mg, 50mg, 100mg) or extended analytical services are also available upon request.

Purity: 80% pure
Qty: 1-5 mgs (large quantity available)
Peptide Length: 7-18 aa
Counter-ion: TFA
QC/QA:100% MALDI MS and HPLC analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 2-3 weeks
Price/peptide: Inquire
Minimum order: 96 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL4000-48-90 has a specified purity of >90% for all peptides in the pool. It is designed as a monitoring tool for vaccination screening for clinical trials. Peptides are produced in a regulated environment according to ISO9001 regulation. These peptides are fully analyzed by MALDI-MS, analytical HPLC and amino acid analysis in quantities of 1-5 mgs per peptide with 7-18 amino acids in length. Longer peptide sizes, higher purity, large quantity (10mg, 20mg, 50mg, 100mg) or extended analytical services are also available upon request.

Purity: 90% pure
Qty: 1-5 mgs (large quantity available)
Peptide Length: 7-18 aa
Counter-ion: TFA
QC/QA:100% MALDI MS, HPLC and amino acid analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time:3-4 weeks
Price/peptide: Inquire
Minimum order: 48 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.

Peptide library PL4000-96-90 has a specified purity of >90% for all peptides in the pool. It is designed as a monitoring tool for vaccination screening for clinical trials. Peptides are produced in a regulated environment according to ISO9001 regulation. These peptides are fully analyzed by MALDI-MS, analytical HPLC and amino acid analysis in quantities of 1-5 mgs per peptide with 7-18 amino acids in length. Longer peptide sizes, higher purity, large quantity (10mg, 20mg, 50mg, 100mg) or extended analytical services are also available upon request.

Purity: 90% pure
Qty: 1-5 mgs (large quantity available)
Peptide Length: 7-18 aa
Counter-ion: TFA
QC/QA:100% MALDI MS, HPLC and amino acid analysis
Formulation: lyophilized in tube or tube-plates
Delivery Time: 3-4 weeks
Price/peptide: Inquire
Minimum order: 96 peptides

* For very hydrophobic peptides yields may be very low. We do not guarantee the purity or success of any one peptide on the same synthesis.


* MS = MALDI mass spectrometry
* AAA = Amino Acid Analysis

List of our ready to go Peptide Arrays, Pools and Libraries

  • Histone Peptide Libraries
  • Mtb Peptide Library
  • CEP Peptide Library
  • Beta Amyloid Peptide Library
  • CD4 T-Cell Restricted Epitope Library

Bio-Synthesis offers seven powerful online peptide library design tools for you to generate large numbers of screening peptides, which involves epitope mapping, sequence optimization, and sequence stabilization. Epitope mapping requires the construction of overlapping peptide libraries, which can be customized by adjusting the fragment length and offset number for the optimum balance between low cost and high data value. In addition to overlapping peptide libraries, Bio-Synthesis offers additional peptide library screening service tools, featuring different strategies for sequence optimization.

overlapping peptide library

Overlapping Peptide Library:

The generation of peptide library by breaking the original protein or peptide into many equal-length overlapping fragments using them for linear or continuous epitope mapping and T-cell epitope determination.

Alanine Peptide Scanning Library:

The generation of peptide library in which alanine (Ala, A) is systematically substituted into each of the amino acids, which can be used to identify epitope activity.

Positional Peptide Library:

A selected position in a peptide sequence is systematically replaced with different amino acid to show the effect on the substitute amino acid at certain position.

Truncation Peptide Library:

The truncation library is been used to predict the minimum amino acid length required for optimum epitope activity.

Random Peptide Library:

It is constructed by substituting selected positions on the original peptide randomly and simultaneously with all other natural amino acids in a shot gun approach with a purpose to elucidate potential alternatives for enhanced peptide activity.

Scrambled Peptide Library:

Scramble Library is constructed by carrying out permutation on the original peptide's sequence. It has the potential to give all possible alternatives and offers and represents the highest degree of variability for peptide library.

T-cell Truncated Peptide Libraries:

Allow the testing of all possible T-cell epitopes across a protein of interest.

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please call 1-972-420-8505 or Fax at 1-972-420-0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."