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PNA FISH Probes

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Fluorescence in situ hybridization (FISH) is a powerful tool in diverse areas of basic research as well as in clinical medicine. This molecular cytogenetic technique has revolutionized our ability to localize specific DNA sequences within interphase chromatin and metaphase chromosomes and to identify both structural and numerical chromosome changes. While FISH is becoming one of the most used cytochemical staining technique in many labs, the new PNA-FISH approach is quickly gaining speed. The method of using DNA analogues to recognize and bind specific nucleic acid sequences in situ to detect chromosomal abnormalities and repeat structure in the human genome have quickly become an important tool in biological assays.

Bio-Synthesis offers on-demand PNA FISH probes in either a red or a green fluorophore (Texas Red, FITC, Cy3, Alexa series respectively). Each FISH probe is supplied in 5 or 10 nmole quantities (100-200 tests), shipped lyophilized and is HPLC purified. Large volume is available, contact us for custom PNA manufacturing.

PNA probes can be used in many hybridization applications as natural or synthetic DNA probes, but PNA probes offer distinctive advantages over DNA probes. First, the neutral backbone allows PNA/DNA binding to occur more rapidly and tightly than DNA/DNA binding at lower ionic strength conditions that disfavor reannealing of complimentary genomic strands. This advantage is particularly important for in situ hybridization experiments which target repetitive sequences. Both the length and the repetitive nature of the target sequences will affectively favor renaturation over hybridization with labeled probes. Second, the high affinity of PNA probes to DNA also constitutes an important feature for chromosomal analysis. As studies have demonstrated, PNA probes can discriminate between two centromeric DNA repeats that differ by only a single base pair1,2, whereas standard FISH probes are unable to discriminate sequences with a single base resolution. Additional benefits of using PNAs in FISH applications are lower background signals and unlimited stability of the probe mixture3. PNA probes are compatible with a wide range of reporter molecules and fluorochromes, including fluoresceine, rhodamine, as well as cyanine and Alexa dyes in a wide variety of colors.

Comparison of PNA and DNA

Criteria PNA DNA
Hybridization affinity with DNA At least 1 °C higher Tm per base -
Hybridization rate with DNA 100 - 5000 times faster -
Salt concentration for hybridization Independent Dependent
Tm for each single mismatch Lowering 15°C Lowering 10°C
Chemical stability Stable Unstable or moderate
Biological stability Stable to nuclease and protease Degradation by nuclease
Thermal stability Good Moderate
Water solubility Restricted solubility enhanced by use of appropriate linkers Soluble
Probe length for diagnostic use 13 - 18 bases 20-30 bases

Reference/Citing:

1.
Chen C, Hong YK, Ontiveros SD, Egholm M, Strauss WM: Single base discrimination of CENP-B repeats on mouse and human chromosomes with PNA-FISH. Mamm Genome 1999; 10:13-18.
2.
Chen C, Wu B, Wei T Egholm M, Strauss WM: Unique chromosome identification and sequence-specific structural analysis with short PNA oligomers. Mamm Genome 2000; 11:384-391
3.
Williams et al.: PNA fluorescent in situ hybridization for rapid microbiology and cytogenetic analysis. Humana Press 2002; 181-193
4.
P. Paulasova et al.: The peptide nucleic acids as probes for chromosomal analysis: application to human oocytes, polar bodies and preimplantation embryos. Molecular Human Reproduction 2004; 10:467-472
5.
D. Griend at al.: Dual-Label Centromere and Telomere FISH Identifies Human, Rat, and Mouse Cell Contribution to Multispecies Recombinant Urogenital Sinus Xenografts. The Prostate 2009; 69:1557-156

Telomere PNA FISH Probes

Telomeres consist of repeats of TTAGGG and CCCTAA sequence at the ends of vertebrate chromosome. These repeats have played an important roles in essential cellular processes like replication, malignant transformation, cellular aging and believed to play a role in senescence and cancer.

Bio-Synthesis provides on-demand Telomere PNA FISH probes as an invaluable tool to researcher seeking solution to detect human telomere sequences using fluorophore-labeled PNA. This method provides excellent quantitation as the fluorescence intensity directly correlates to the length of the telomeres.

Bio-Synthesis offers on-demand Telomere PNA FISH probes in either a red or a green fluorophore (Texas Red, FITC, FAM, TAMRA Cy3, Alexa series respectively). Each FISH probe is supplied in 5 or 10 nmole quantities (100-200 tests), shipped lyophilized and HPLC purified. Large volume is available, contact us for custom PNA manufacturing.

Telomere C FISH Probe

Item No. Product Name Sequences Quantity Price
PNA1001-005 PNA Telomere C FISH FAM Probe FAM-OO-(CCCTAA)3 5 nmoles $245
PNA1001-010 PNA Telomere C FISH FAM Probe FAM-OO-(CCCTAA)3 10 nmoles $395
PNA1002-005 PNA Telomere C FISH FITC Probe FITC-OO-(CCCTAA)3 5 nmoles $300
PNA1002-010 PNA Telomere C FISH FITC Probe FITC-OO-(CCCTAA)3 10 nmoles $500
PNA1003-005 PNA Telomere C FISH TAMRA Probe TAMRA-OO-(CCCTAA)3 5 nmoles $300
PNA1003-010 PNA Telomere C FISH TAMRA Probe TAMRA-OO-(CCCTAA)3 10 nmoles $500
PNA1004-005 PNA Telomere C FISH CY3 Probe CY3-OO-(CCCTAA)3 5 nmoles $300
PNA1004-010 PNA Telomere C FISH CY3 Probe CY3-OO-(CCCTAA)3 10 nmoles $500
PNA1005-005 PNA Telomere C FISH CY5 Probe CY5-OO-(CCCTAA)3 5 nmoles $300
PNA1005-010 PNA Telomere C FISH CY5 Probe CY5-OO-(CCCTAA)3 10 nmoles $500
PNA1006-005 PNA Telomere C FISH Alexa 488 Probe Alexa 488-OO-(CCCTAA)3 5 nmoles $450
PNA1006-010 PNA Telomere C FISH Alexa 488 Probe Alexa 488-OO-(CCCTAA)3 10 nmoles $700
PNA1007-005 PNA Telomere C FISH Alexa 555 Probe Alexa 555-OO-(CCCTAA)3 5 nmoles $450
PNA1007-010 PNA Telomere C FISH Alexa 555 Probe Alexa 555-OO-(CCCTAA)3 10 nmoles $700
PNA1008-005 PNA Telomere C FISH Alexa 610 Probe Alexa 610-OO-(CCCTAA)3 5 nmoles $450
PNA1008-010 PNA Telomere C FISH Alexa 610 Probe Alexa 610-OO-(CCCTAA)3 10 nmoles $700
PNA1009-005 PNA Telomere C FISH Alexa 647 Probe Alexa 647-OO-(CCCTAA)3 5 nmoles $450
PNA1009-010 PNA Telomere C FISH Alexa 647 Probe Alexa 647-OO-(CCCTAA)3 10 nmoles $700
PNA1010-005 PNA Telomere C FISH Texas Red Probe Texas Red-OO-(CCCTAA)3 5 nmoles $450
PNA1010-010 PNA Telomere C FISH Texas Red Probe Texas Red-OO-(CCCTAA)3 10 nmoles $700
PNA1011-005 PNA Telomere C FISH Biotin Probe Biotin-OO-(CCCTAA)3 5 nmoles $300
PNA1011-010 PNA Telomere C FISH Biotin Probe Biotin-OO-(CCCTAA)3 10 nmoles $500
PNA1012-005 PNA Telomere C FISH Unlabeled Probe OO-(CCCTAA)3 5 nmoles $180
PNA1012-010 PNA Telomere C FISH Unlabeled Probe OO-(CCCTAA)3 10 nmoles $220
PNA1013-005 PNA Telomere C FISH Naked Sequence (CCCTAA)3 5 nmoles $150
PNA1013-010 PNA Telomere C FISH Naked Sequence (CCCTAA)3 10 nmoles $200

Telomere G FISH Probe

Item No. Product Name Product Description Quantity
Price
PNA1050-005 PNA Telomere G FISH FAM Probe FAM-OO-(TTAGGG)3 5 nmoles $245
PNA1050-010 PNA Telomere G FISH FAM Probe FAM-OO-(TTAGGG)3 10 nmoles $395
PNA1051-005 PNA Telomere G FISH FITC Probe FITC-OO-(TTAGGG)3 5 nmoles $300
PNA1051-010 PNA Telomere G FISH FITC Probe FITC-OO-(TTAGGG)3 10 nmoles $500
PNA1052-005 PNA Telomere G FISH TAMRA Probe TAMRA-OO-(TTAGGG)3 5 nmoles $300
PNA1052-010 PNA Telomere G FISH TAMRA Probe TAMRA-OO-(TTAGGG)3 10 nmoles $500
PNA1053-005 PNA Telomere G FISH CY3 Probe CY3-OO-(TTAGGG)3 5 nmoles $300
PNA1053-010 PNA Telomere G FISH CY3 Probe CY3-OO-(TTAGGG)3 10 nmoles $500
PNA1054-005 PNA Telomere G FISH CY5 Probe CY5-OO-(TTAGGG)3 5 nmoles $300
PNA1054-010 PNA Telomere G FISH CY5 Probe CY5-OO-(TTAGGG)3 10 nmoles $500
PNA1055-005 PNA Telomere G FISH Alexa 488 Probe Alexa 488-OO-(TTAGGG)3 5 nmoles $450
PNA1055-010 PNA Telomere G FISH Alexa 488 Probe Alexa 488-(TTAGGG)3 10 nmoles $700
PNA1056-005 PNA Telomere G FISH Alexa 555 Probe Alexa 555-OO-(TTAGGG)3 5 nmoles $450
PNA1056-010 PNA Telomere G FISH Alexa 555 Probe Alexa 555-OO-(TTAGGG)3 10 nmoles $700
PNA1057-005 PNA Telomere G FISH Alexa 610 Probe Alexa 610-OO-(TTAGGG)3 5 nmoles $450
PNA1057-010 PNA Telomere G FISH Alexa 610 Probe Alexa 610-OO-(TTAGGG)3 10 nmoles $700
PNA1058-005 PNA Telomere G FISH Alexa 647 Probe Alexa 647-OO-(TTAGGG)3 5 nmoles $450
PNA1058-010 PNA Telomere G FISH Alexa 647 Probe Alexa 647-OO-(TTAGGG)3 10 nmoles $700
PNA1059-005 PNA Telomere G FISH Texas Red Probe Texas Red-OO-(TTAGGG)3 5 nmoles $450
PNA1059-010 PNA Telomere G FISH Texas Red Probe Texas Red-OO-(TTAGGG)3 10 nmoles $700
PNA1060-005 PNA Telomere G FISH Biotin Probe Biotin-OO-(TTAGGG)3 5 nmoles $300
PNA1060-010 PNA Telomere G FISH Biotin Probe Biotin-OO-(TTAGGG)3 10 nmoles $500
PNA1061-005 PNA Telomere G FISH Unlabeled Probe OO-(TTAGGG)3 5 nmoles $180
PNA1061-010 PNA Telomere G FISH Unlabeled Probe OO-(TTAGGG)3 10 nmoles $220
PNA1062-005 PNA Telomere G FISH Naked Sequence (TTAGGG)3 5 nmoles $150
PNA1062-010 PNA Telomere G FISH Naked Sequence (TTAGGG)3 10 nmoles $200

Centromere PNA FISH Probes

Centromeres are special structures of eukaryotic chromosomes that hold sister chromatid together and ensure proper chromosome segregation during cell division. These repeated sequences play an important role in centromere mitotic recombination and its consequences for centromeric function. PNA-FISH centromere probes have been used to visualize and quantify directed against aberration in these interspersed alpha-satellite chromosomal related diseases, such as, aneuploidies (trisomies), polysomy in human.

Bio-Synthesis offers on-demand centromere PNA FISH probes in either a red or a green fluorophore (FAM, FITC, TAMRA, Cy3, Alexa series respectively). Each FISH probe is supplied in 5 or 10 nmole quantities (100-200 tests), shipped lyophilized and HPLC purified. Large volume is available, contact us for custom PNA manufacturing.

Centromere FISH Probe (Human)

Prod. No. Human Centromere FISH Probe Product Description Quantity Price
PNA1100-005 PNA Centromere FISH FAM Probe FAM-OO-ATTCGTTGGAAACGGGA 5 nmoles $245
PNA1100-010 PNA Centromere FISH FAM Probe FAM-OO-ATTCGTTGGAAACGGGA 10 nmoles $395
PNA1101-005 PNA Centromere FISH FITC Probe FITC-OO-ATTCGTTGGAAACGGGA 5 nmoles $300
PNA1101-010 PNA Centromere FISH FITC Probe FITC-OO-ATTCGTTGGAAACGGGA 10 nmoles $500
PNA1102-005 PNA Centromere FISH TAMRA Probe TAMRA-OO-ATTCGTTGGAAACGGGA 5 nmoles $300
PNA1102-010 PNA Centromere FISH TAMRA Probe TAMRA-OO-ATTCGTTGGAAACGGGA 10 nmoles $500
PNA1103-005 PNA Centromere FISH CY3 Probe CY3-OO-ATTCGTTGGAAACGGGA 5 nmoles $300
PNA1103-010 PNA Centromere FISH CY3 Probe CY3-OO-ATTCGTTGGAAACGGGA 10 nmoles $500
PNA1104-005 PNA Centromere FISH CY5 Probe CY5-OO-ATTCGTTGGAAACGGGA 5 nmoles $300
PNA1104-010 PNA Centromere FISH CY5 Probe CY5-OO-ATTCGTTGGAAACGGGA 10 nmoles $500
PNA1105-005 PNA Centromere FISH Alexa 488 Probe Alexa488-OO-ATTCGTTGGAAACGGGA 5 nmoles $450
PNA1105-010 PNA Centromere FISH Alexa 488 Probe Alexa488-OO-ATTCGTTGGAAACGGGA 10 nmoles $700
PNA1106-005 PNA Centromere FISH Alexa 555Probe Alexa 555-OO-ATTCGTTGGAAACGGGA 5 nmoles $450
PNA1106-010 PNA Centromere FISH Alexa 555Probe Alexa 555-OO-ATTCGTTGGAAACGGGA 10 nmoles $700
PNA1107-005 PNA Centromere FISH Alexa 610 Probe Alexa 610-OO-ATTCGTTGGAAACGGGA 5 nmoles $450
PNA1107-010 PNA Centromere FISH Alexa 610 Probe Alexa 610-OO-ATTCGTTGGAAACGGGA 10 nmoles $700
PNA1108-005 PNA Centromere FISH Alexa 647 Probe Alexa 647-OO-ATTCGTTGGAAACGGGA 5 nmoles $450
PNA1108-010 PNA Centromere FISH Alexa 647 Probe Alexa 647-OO-ATTCGTTGGAAACGGGA 10 nmoles $700
PNA1109-005 PNA Centromere FISH Texas Red Probe Texas Red-OO-ATTCGTTGGAAACGGGA 5 nmoles $450
PNA1109-010 PNA Centromere FISH Texas Red Probe Texas Red-OO-ATTCGTTGGAAACGGGA 10 nmoles $700
PNA1110-005 PNA Centromere FISH Biotin Probe Biotin-OO-ATTCGTTGGAAACGGGA 5 nmoles $300
PNA1110-010 PNA Centromere FISH Biotin Probe Biotin-OO-ATTCGTTGGAAACGGGA 10 nmoles $500
PNA1111-005 PNA Centromere FISH Unlabeled Probe OO-ATTCGTTGGAAACGGGA 5 nmoles $180
PNA1111-010 PNA Centromere FISH Unlabeled Probe OO-ATTCGTTGGAAACGGGA 10 nmoles $220
PNA1112-005 PNA Centromere FISH Naked Sequence ATTCGTTGGAAACGGGA 5 nmoles $150
PNA1112-010 PNA Centromere FISH Naked Sequence ATTCGTTGGAAACGGGA 10 nmoles $200

Centromere FISH Probe (Mouse)

Prod. No. Mouse Centromere FISH Probe Product Description Quantity Price
PNA1150-005 Mouse PNA Centromere FISH FAM Probe FAM-OO-CACAAAGAAGTTTCTGAG 5 nmoles $245
PNA1150-010 Mouse PNA Centromere FISH FAM Probe FAM-OO-CACAAAGAAGTTTCTGAG 10 nmoles $395
PNA1151-005 Mouse PNA Centromere FISH FITC Probe FITC-OO-CACAAAGAAGTTTCTGAG 5 nmoles $300
PNA1151-010 Mouse PNA Centromere FISH FITC Probe FITC-OO-CACAAAGAAGTTTCTGAG 10 nmoles $500
PNA1152-005 Mouse PNA Centromere FISH TAMRA Probe TAMRA-OO-CACAAAGAAGTTTCTGAG 5 nmoles $300
PNA1152-010 Mouse PNA Centromere FISH TAMRA Probe TAMRA-OO-CACAAAGAAGTTTCTGAG 10 nmoles $500
PNA1153-005 Mouse PNA Centromere FISH CY3 Probe CY3-OO-CACAAAGAAGTTTCTGAG 5 nmoles $300
PNA1153-010 Mouse PNA Centromere FISH CY3 Probe CY3-OO-CACAAAGAAGTTTCTGAG 10 nmoles $500
PNA1154-005 Mouse PNA Centromere FISH CY5 Probe CY5-OO-CACAAAGAAGTTTCTGAG 5 nmoles $300
PNA1154-010 Mouse PNA Centromere FISH CY5 Probe CY5-OO-CACAAAGAAGTTTCTGAG 10 nmoles $500
PNA1155-005 Mouse PNA Centromere FISH Alexa488 Probe Alexa 488-OO-CACAAAGAAGTTTCTGAG 5 nmoles $450
PNA1155-010 Mouse PNA Centromere FISH Alexa488 Probe Alexa 488-OO-CACAAAGAAGTTTCTGAG 10 nmoles $700
PNA1156-005 Mouse PNA Centromere FISH Alexa 555 Probe Alexa 555-OO-CACAAAGAAGTTTCTGAG 5 nmoles $450
PNA1156-010 Mouse PNA Centromere FISH Alexa 555 Probe Alexa 555-OO-CACAAAGAAGTTTCTGAG 10 nmoles $700
PNA1157-005 Mouse PNA Centromere FISH Alexa 610 Probe Alexa 610-OO-CACAAAGAAGTTTCTGAG 5 nmoles $450
PNA1157-010 Mouse PNA Centromere FISH Alexa 610 Probe Alexa 610-OO-CACAAAGAAGTTTCTGAG 10 nmoles $700
PNA1158-005 Mouse PNA Centromere FISH Alexa 647 Probe Alexa 647-OO-CACAAAGAAGTTTCTGAG 5 nmoles $450
PNA1158-010 Mouse PNA Centromere FISH Alexa 647 Probe Alexa 647-OO-CACAAAGAAGTTTCTGAG 10 nmoles $700
PNA1159-005 Mouse PNA Centromere FISH Texas Red Probe Texas Red-OO-CACAAAGAAGTTTCTGAG 5 nmoles $450
PNA1159-010 Mouse PNA Centromere FISH Texas Red Probe Texas Red-OO-CACAAAGAAGTTTCTGAG 10 nmoles $700
PNA1160-005 Mouse PNA Centromere FISH Biotin Probe Biotin-OO-CACAAAGAAGTTTCTGAG 5 nmoles $300
PNA1160-010 Mouse PNA Centromere FISH Biotin Probe Biotin-OO-CACAAAGAAGTTTCTGAG 10 nmoles $500
PNA1161-005 Mouse PNA Centromere FISH Unlabeled Probe OO-CACAAAGAAGTTTCTGAG 5 nmoles $180
PNA1161-010 Mouse PNA Centromere FISH Unlabeled Probe OO-CACAAAGAAGTTTCTGAG 10 nmoles $220
PNA1162-005 Mouse PNA Centromere FISH Naked Sequence CACAAAGAAGTTTCTGAG 5 nmoles $150
PNA1162-010 Mouse PNA Centromere FISH Naked Sequence CACAAAGAAGTTTCTGAG 10 nmoles $200

Links and Resources



Protocol:


Protocol for PNA Telomere Probe for FISH Application

Useful Links

Reference/Citing:

1.
S. Selvaraju et al.: Peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) assay for specific detection of Mycobacterium immunogenum and DNA-FISH assay for analysis of pseudomonads in metalworking fluids and sputum. Molecular and Cellular Probes 2008, Vol 22, Issue 5-6, P273-280
2.
E. Akbay et al.: Differential Roles of Telomere Attrition in Type I and II Endometrial Carcinogenesis. American Society for Investigative Pathology 2008, 173:536-544
3.
B Steensel at al: TRF2 Protects Human Telomeres from End-to-End Fusions. Cell 1998, Vol. 92, 401-413.
4.
J. Kenny at al.: Detection of viable Yersinia pestis by fluorescence in situ hybridization using peptide nucleic acid probes, Journal of Microbiological Methods 2008 Vol 75, Issue 2, P293-301
5.
A. Meeker at al.: Telomere Length Assessment in Human Archival Tissues Combined Telomere Fluorescence in Situ Hybridization and Immunostaining. American Society for Investigative Pathology 2002, 160(4): 1259-1268
6.
K. Shimizu-Kohno at al.: Detection of HTLV-1 by means of HBZ gene in situ hybridization in formalin-fixed and paraffin-embedded tissues. Cancer Science 102 (7):1432-1436
7.
E. Sloand at al.: T cell immune responses to Wilms tumor 1 protein in myelodysplasia responsive to immunosuppressive therapy. Blood 2010
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