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Secondary Antibody Detection Probes
Secondary Antibody Detection Probes

Custom Secondary Antibody Labeling Probes

The interactions of biotin and biotin binding proteins have been a useful tool in protein and nucleic detection and purification. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and used as primary probes for detection with avidin or streptavidin "secondary" probes, or other detection reagents for use in biological assays.

Conjugates of high-quality and reproducible secondary antibodies are essential for consistent experimental results. Bio-Synthesis provides Custom Secondary Antibodies Detection Probes using state-of-the-art conjugation chemistries and purification methods with the ability to label microgram to milligram amounts of your antibody in a short amount of time.

We offer custom conjugation of these secondary probes by request:

  • Fluorescent Labeling
  • Microsphere Labeling
  • Qdot Conjugates
  • Enzyme Conjugation of Avidin and Streptavidin
  • Gold-Labeled Streptavidin
  • Streptavidin Agarose Conjugation
  • CaptAvidin Agarose Conjugation
  • Acrylamide Conjugates for Immobilization of Avidins in Polymers
  • Anti-Biotin Antibody: An Alternative to Avidins

To obtain further Information, please contact us.

The following is a list of our currently available custom antibody detection probe services. Should you not find a service that suits your needs, we will find a solution for you!

We can conjugate:

  • Secondary Antibodies: Anti-mouse, Anti-rabbit, anti-chicken, Anti-bovine conjugation, etc.
  • Biotin-Binding Proteins (Avidin, NeutrAvidin®, Streptavidin,etc.)
  • Antibody-Binding Protein (Protein A, G, etc.)
Services Application
Avidin and NeutrAvidin®
Avidin, NeutrAvidin® TRITC conjugation IHCP, IHCF, IC, IF
Avidin, NeutrAvidin® FITC conjugation IHCP, IHCF, IC, FCM, IF
Avidin, Alexa Fluor® 488 conjugation IHCP, IHCF, IC, FCM, IF
Avidin, NeutrAvidin®, R-phycoerythrin conjugation IHCP, IHCF, IC, FCM, IF
Avidin, NeutrAvidin®, Cascade Blue® conjugation IHCP, IHCF, IC, FCM, IF
Avidin, NeutrAvidin®, Texas Red® conjugation IHCP, IHCF, IC, FCM, IF
Avidin, NeutrAvidin® biotin-binding protein conjugation IHCP, IHCF, IC, FCM, IF
Avidin, egg white conjugation IHCP, IHCF, IC, FCM, IF
Avidin, NeutrAvidin®, tetramethylrhodamine conjugation IHCP, IHCF, IC, FCM, IF
Avidin, NeutrAvidin®, Oregon Green® 488 conjugation IHCP, IHCF, IC, FCM, IF
Avidin, NeutrAvidin®, Rhodamine RedTM-X conjugation IHCP, IHCF, IC, FCM, IF
Avidin Antibody, HRP conjugations (Avidin as target protein) WB IHCP, IHCF, Dot Blot
Avidin, NeutrAvidin® HRP conjugations WB IHCP, IHCF, Dot Blot
Avidin, NeutrAvidin® Colloidal gold (5 nm) conjugations IHCP, IHCF, EM
Avidin, NeutrAvidin®, HRP conjugations WB IHCP, IHCF, Dot Blot
Avidin NeutrAvidin, Alkaline Phosphatase (AP) conjugations WB, IHC, in situ Hybridization, ELISA
Steptavidin
Alexa Fluor® 488 streptavidin, 5 nm colloidal gold conjugation IHCP, IHCF, EM,WB
Alexa Fluor® 488 streptavidin, 10 nm colloidal gold conjugation IHCP, IHCF, EM,WB
Streptavidin, Alexa Fluor® 488 FluoroNanogoldTM conjugation IHCP, IHCF, EM,WB
Streptavidin, Alexa Fluor® 594 FluoroNanogoldTM conjugation IHCP, IHCF, EM,WB
Streptavidin, Marina Blue® conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Pacific BlueTM conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 488 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 532 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 546 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 568 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 594 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Cascade YellowTM conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 430 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 350 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 610-R-phycoerythrin conjugation (Alexa Fluor® 610-R-phycoerythrin Streptavidin) IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 680-R-phycoerythrin conjugation (Alexa Fluor® 680-R-phycoerythrin Streptavidin) IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 647-R-phycoerythrin conjugation (Alexa Fluor® 647-R-phycoerythrin Streptavidin) IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 680-allophycocyanin conjugation (Alexa Fluor® 680-allophycocyanin Streptavidin) IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 700-allophycocyanin conjugation (Alexa Fluor® 700-allophycocyanin Streptavidin) IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 750-allophycocyanin conjugation (Alexa Fluor® 750-allophycocyanin Streptavidin) IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 647 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 633 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 660 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 680 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin acrylamide conjugation Biosensors
Streptavidin, Alexa Fluor® 555 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 700 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 750 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, R-phycoerythrin conjugation (SAPE) IHCP, IHCF, IC, FCM, IF
Streptavidin, β-lactamase TEM-1 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, B-phycoerythrin conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 405 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 514 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 488 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 555 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 594 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 647 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 680 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, allophycocyanin conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 750-R-phycoerythrin conjugation (Alexa Fluor® 750-R-phycoerythrin Streptavidin) IHCP, IHCF, IC, FCM, IF
Streptavidin, Alexa Fluor® 635 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Pacific OrangeTM conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Rhodamine RedTM-X conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Oregon Green® 488 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Oregon Green® 514 conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Texas Red®-X conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, R-phycoerythrin conjugation (SAPE) IHCP, IHCF, IC, FCM, IF
Streptavidin, FITC Conjugation IHCP, IHCF, IC, FCM, IF, IM
Streptavidin, Tetramethylrhodamine Conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Rhodamine B Conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, Texas Red® Conjugation IHCP, IHCF, IC, FCM, IF
Streptavidin, HRP conjugations WB, IHCP, IHCF, IM, Dot Blot
Streptavidin, Alkaline Phosphatase (AP) conjugations WB, ELISA, IHC,
Streptavidin, β-galactosidase conjugation (BGAL) EIA, IHC, ELISA, IC, TSA,
Protein A
Protein A, FITC conjugations IHCP, IHCF, IC, FCM, IF
Protein A, HRP conjugations WB IHCP, IHCF, Dot Blot
Protein A, Alkaline Phosphatase conjugations (AP) ELISA, WB
Protein A, Colloidal gold (10 nm) conjugations IHCP, IHCF, EM
Protein A Colloidal gold (5 nm) conjugations IHCP, IHCF, EM

Binding Characteristics of Biotin-Binding Proteins

Streptavidin, avidin and NeutrAvidin biotin-binding proteins each bind four biotins per molecule with high affinity and specificity. Their multiple binding sites permit a number of techniques in which unlabeled avidin, streptavidin or NeutrAvidin biotin-binding protein can be used to bridge two biotinylated reagents. This bridging method, which is commonly used to link a biotinylated probe to a biotinylated enzyme in enzyme-linked immunohistochemical applications, often eliminates background problems that can occur when using direct avidin or streptavidin-enzyme conjugates. The table below is a comparison of each binding protein we offer.

We offer three different kinds of biotin-binding proteins:

Properties of Biotin-Binding Proteins
  Avidin Streptavidin NeutrAvidin Protein
Molecular Weight 67K 53K 60K
Biotin-binding Sites 4 4 4
Isoelectric Point (pl) 10.5 6.8-7.5 6.3
Specificity Low High Highest
Affinity for Biotin (Kd) 10-15 M 10-15 M 10-15 M
Nonspecific Binding High Low Lowest

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please call 1-972-420-8505 or Fax at 1-972-420-0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."