Fluorophore Antibody Conjugation
Labeling antibodies with fluorochromes provides a colored reagent that can be directly observed. The intensity and narrow wavelength of the fluorochromes makes fluorochromes useful tools in immunochemistry. Many of our labels can be used with multiple antibodies that do not cross-react to create custom multiplexed assays.
We are experienced in performing both small-scale and large-scale conjugations and purifications - let us put our expertise to work for you.
We can conjugate antibodies/proteins to many labels, including:
- Alexa Fluor dyes
- Qdot nanocrystals
- ATTO Fluor dyes
- Dylight series
- QCy dyes
- Cascade Blue, Oregon Green, and other Molecular Probes proprietary fluorophores
- Traditional fluorophores - e.g. FITC, TRITC, Texas Red
- Biotins and other haptens
- R-PE, APC and Alexa Fluor tandems
- Horseradish peroxidase, alkaline phosphatase and other enzymes
Don't see the label you are interested in? Just ask. In most cases we can accommodate your request. Consult our experts if you need assistance in choosing the optimal fluorophore for your conjugation.
Cost of Service
For standard antibody conjugations to organic dyes, please contact us for a quote.
To initiate a Custom Antibody Labeling Project:
Contact us to initiate a Custom Antibody Labeling Project. BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting customer specifications.
We will make our best effort to produce the conjugate you request. However, because optimal labeling must be determined empirically, we cannot guarantee that a single conjugation will produce the best product for your needs. Please note that we cannot guarantee the final yield or that the final product will be biologically active. We do not refund payments for services diligently performed.
*Trademarks and Patents
Spectral Red and Fluoromount-G® are trademarks of Southern Biotechnology Associates, Inc.
Spectral Red is a PE-CY5 tandem conjugate.
The following are trademarks of other companies mentioned or referenced in this catalog:
FACScanTM is a registered trademark of Becton Dickinson Immunocytometry Systems, Inc.
VectaStainTM and ABCTM are registered trademarks of Vector Laboratories.
*Cy5 is a trademark of Amersham Biosciences Corp. Cy5 is for non-commercial research use only, not for therapeutic or in vivo applications.
R-phycoerythrin (R-PE) is protected by US Patent Nos. 4,520,114 and 4,524,104, European Patent No. 0076695, and Canadian Patent No. 1,179,942.
Cy3 and Cy5 fluorescent probes are used under license from Amersham Life Sciences, Inc., U.S. Patent Nos. 4,981,977 and 5,268,486, and other patents pending. This material (or portions of this material) is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. There is no implied license hereunder for any commercial use. Commercial Use shall include; 1. sale, lease, license or other transfer of the material or any material derived or produced from it. 2. sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3. use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, return this material, unopened to SouthernBiotech, 160A Oxmoor Blvd, Birmingham, AL 35209, USA, and any money paid for the material will be refunded.
Don't see the label you are interested in? Just ask, in most cases we can accommodate your request.
And if you require help in choosing the optimal fluorophore for your conjugation, you can consult our experts.
Antibodies should be supplied at a minimum concentration of 5 mg/ml in neutral buffer without preservatives or protease inhibitors.