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Antibody Fluorescent Labeling

Antibody Fluorescent Labeling

Antibody Fluorescent Dye Labeling

Fluorescent labeling for antibodies have become a common tool in laboratories for visualizing and studying proteins, whether they are attached directly to the protein of interest or to antibodies specific for the protein of interest. The intensity and narrow wavelength of the fluorochromes makes them useful tools in immunohistochemistry and immunocytochemistry. Bio-Synthesis offers high quality, efficient and cost-saving antibody fluorescent labeling services between antibody/protein, phycobiliproteins (RPE, APC, cl-APC), streptavidin, and biotin, with a wide selection of fluorescent dyes. Since every antibody/protein has its own unique characteristics, conjugation needs to be optimized. Antibody/protein of interest will be subject to quality check by HPLC prior to conjugation. We will complete the conjugation within 1-2 weeks from the time we receive the sample.

Choosing the best antibody fluorophore conjugate for your application is not always easy. We can help you decide on the most suitable conjugate for your applications. Below is a list of our currently available custom antibody fluorescent labeling services. Should you not find a service that suits your needs, we will find a solution for you!

To obtain further information, contact us.

Fluorophore Antibody Conjugation

Labeling antibodies with fluorochromes provides a colored reagent that can be directly observed. The intensity and narrow wavelength of the fluorochromes makes fluorochromes useful tools in immunochemistry. Many of our labels can be used with multiple antibodies that do not cross-react to create custom multiplexed assays.

We are experienced in performing both small-scale and large-scale conjugations and purifications - let us put our expertise to work for you.

We can conjugate antibodies/proteins to many labels, including:

  • Alexa Fluor dyes
  • Qdot nanocrystals
  • ATTO Fluor dyes
  • Dylight series
  • QCy dyes
  • Cascade Blue, Oregon Green, and other Molecular Probes proprietary fluorophores
  • Traditional fluorophores - e.g. FITC, TRITC, Texas Red
  • Biotins and other haptens
  • R-PE, APC and Alexa Fluor tandems
  • Horseradish peroxidase, alkaline phosphatase and other enzymes

Don't see the label you are interested in? Just ask. In most cases we can accommodate your request. Consult our experts if you need assistance in choosing the optimal fluorophore for your conjugation.

Cost of Service

For standard antibody conjugations to organic dyes, please contact us for a quote.

To initiate a Custom Antibody Labeling Project:

Contact us to initiate a Custom Antibody Labeling Project. BSI supplies a Polyclonal Antibody Information Sheet with each inquiry to assist in meeting customer specifications.

We will make our best effort to produce the conjugate you request. However, because optimal labeling must be determined empirically, we cannot guarantee that a single conjugation will produce the best product for your needs. Please note that we cannot guarantee the final yield or that the final product will be biologically active. We do not refund payments for services diligently performed.

*Trademarks and Patents

Spectral Red and Fluoromount-G® are trademarks of Southern Biotechnology Associates, Inc.

Spectral Red is a PE-CY5 tandem conjugate.

The following are trademarks of other companies mentioned or referenced in this catalog:

FACScanTM is a registered trademark of Becton Dickinson Immunocytometry Systems, Inc.

VectaStainTM and ABCTM are registered trademarks of Vector Laboratories.

*Cy5 is a trademark of Amersham Biosciences Corp. Cy5 is for non-commercial research use only, not for therapeutic or in vivo applications.

R-phycoerythrin (R-PE) is protected by US Patent Nos. 4,520,114 and 4,524,104, European Patent No. 0076695, and Canadian Patent No. 1,179,942.

Cy3 and Cy5 fluorescent probes are used under license from Amersham Life Sciences, Inc., U.S. Patent Nos. 4,981,977 and 5,268,486, and other patents pending. This material (or portions of this material) is subject to proprietary rights of Amersham Biosciences Corp. and Carnegie Mellon University and made and sold under license from Amersham Biosciences Corp. This product is licensed for sale only for research. It is not licensed for any other use. There is no implied license hereunder for any commercial use. Commercial Use shall include; 1. sale, lease, license or other transfer of the material or any material derived or produced from it. 2. sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3. use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, return this material, unopened to SouthernBiotech, 160A Oxmoor Blvd, Birmingham, AL 35209, USA, and any money paid for the material will be refunded.

Don't see the label you are interested in? Just ask, in most cases we can accommodate your request.

And if you require help in choosing the optimal fluorophore for your conjugation, you can consult our experts.

Antibody-Dye Labels

Antibodies should be supplied at a minimum concentration of 5 mg/ml in neutral buffer without preservatives or protease inhibitors.

Label Ex/Em Emt Color* Recommended Use
Alexa Fluor® 350 346/442 Imaging
Alexa Fluor® 488 495/519 Flow cytometry and imaging
Alexa Fluor® 532 532/553 Imaging
Alexa Fluor® 546 556/573 Imaging
Alexa Fluor® 555 555/565 Imaging
Alexa Fluor® 568 578/603 Imaging
Alexa Fluor® 594 590/617 Imaging
Alexa Fluor® 633 621/639 Flow cytometry and imaging
Alexa Fluor® 647 650/665 Flow cytometry and imaging
Alexa Fluor® 660 663/690 Flow cytometry and imaging
Alexa Fluor® 680 679/702 Imaging
Dylight® 405 400/420 Imaging
Dylight® 488 493/518 Flow cytometry and imaging
Dylight® 549 562/576 Imaging
Dylight® 594 593/618 Imaging
Dylight® 633 638/658 Flow cytometry and imaging
Dylight® 649 654/673 Flow cytometry and imaging
Dylight® 680 692/712 Imaging
Dylight® 750 752/778
Dylight® 800 777/794
Cy3 550/565 Fluorescent microscopy
Cy3.5 581/596 Fluorescent microscopy
Cy5 Immunoblot includes: Amersham Brand Typhoon® System, and immunoflourescent applications
Cy5.5 Immunoblot, especially for Li-COR Odyssey systems
Oregon Green® 488 496/524 Flow cytometry and imaging
Fluorescein (i.e. FITC) 494/518 Flow cytometry, ind, BD FACS systems, Guava system, and fluorescent microscopy
Pacific Blue™ dye 410/455 Flow cytometry and imaging
Pacific Orange™ dye 400/551 Flow cytometry
Allophycocyanin (APC) 652/670 FRET assays and HTRF assays
Rhodamine 6G 525/555 Flow cytometry, immunoblots, and fluorescent microscopy
Rhodamine Green 502/527 Fluorescent microscopy
Rhodamine Red 560/580 Flow cytometry, fluorescent microscopy
R-Phycoerythrin (PE) (495) 565/575 Flow cytometry, Lumiex® and Guava® systems, FRET assays, and capillary electrophoresis; use with FITC for double labeling
Texas Red* Sulfonyl Chloride 595/615 Imaging
Biotin n/a Flow cytometry and other fluorescent applications

Reference/Citing:

  1. Different prelamin A forms accumulate in human fibroblasts: a study in experimental models and progeria
  2. 1,25-DIHYDROXYVITAMIN D3-INDUCED GENES IN OSTEOBLASTS: UNCOVERING NEW FUNCTIONS FOR MENINGIOMA 1 AND SEMAPHORIN 3B IN SKELETAL PHYSIOLOGY
  3. Fetal Death in Mice Lacking 5α-Reductase Type 1 Caused by Estrogen Excess
  4. A splicing variant of a death domain protein that is regulated by a mitogen-activated kinase is a substrate for c-Jun N-terminal kinase in the human central nervous system

Visit Literature Vault for more citing on Custom Polyclonal Antibodies

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please call 1-972-420-8505 or Fax at 1-972-420-0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."