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Antibody Characterization Services

Custom antibody characterization services to evaluate quantity, purity, identity, and functional performance of polyclonal and monoclonal antibodies. Support includes ELISA titration and quantitation, Western blot analysis, HPLC / SDS-PAGE / IEF, intact mass determination, N- and C-terminal sequencing, and murine isotype determination.

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Overview Capabilities Methods Workflow Sample Requirements Deliverables FAQ Contact

Overview

Antibody characterization is essential for determining whether an antibody is suitable for its intended research, assay, or downstream development use. A strong characterization workflow helps confirm quantity, purity, molecular identity, immunoreactivity, and key performance attributes before the antibody moves into labeling, bioconjugation, assay development, or broader analytical programs.

Bio-Synthesis provides characterization support for polyclonal and monoclonal antibodies using a combination of immunoassays, electrophoretic methods, chromatography, mass spectrometry, sequencing, and isotype testing. Services can be selected individually or combined into a project-specific workflow depending on the question you need answered.

ELISA titration & quantitation Western blot assay HPLC / SDS-PAGE / IEF Intact mass analysis Terminal sequencing Murine isotyping Protocol transfer available
Built around your project: Whether you need rapid titer screening, molecular confirmation, purity review, or deeper identity testing, we help align the characterization plan with your sample type, development stage, and downstream application.

Measures

Quantity to identity

Titer, immunoreactivity, purity, intact mass, sequence endpoints, and isotype

Formats

Polyclonal & monoclonal

Support for common antibody development and testing programs

Methods

Orthogonal analysis

ELISA, blotting, electrophoresis, chromatography, mass spectrometry, and sequencing

Output

Actionable data

Results intended to support development decisions and downstream use

Core Capabilities

ELISA Titration & Quantitation

Screen and quantify antibody binding using ELISA-based methods suitable for evaluation of larger sample sets in a rapid and cost-effective format.

Western Blot Assay

Assess immunoreactivity against denatured protein targets and review expected heavy- and light-chain banding patterns as part of identity and purity evaluation.

HPLC, SDS-PAGE & IEF Analysis

Use complementary analytical methods to review purity, heterogeneity, and electrophoretic behavior across antibody samples.

Intact Mass Determination

Mass spectrometry-based confirmation of heavy and light chain molecular mass for stronger identity assessment.

N- and C-Terminal Sequencing

Terminal sequencing support using Edman degradation and MALDI MS approaches for deeper characterization projects.

Murine Isotype Determination

Determine antibody isotype for murine samples in supernatants or ascites using established isotyping workflows.

Characterization Methods

Immunoassay & Functional Readout

  • Antibody titration by ELISA
  • Antibody quantitation by ELISA
  • ELISA development and sample testing
  • Protocol transfer for validated ELISA workflows
  • Western blot immunoreactivity analysis

Analytical & Structural Evaluation

  • HPLC analysis
  • SDS-PAGE and IEF analysis
  • Intact mass determination by mass spectrometry
  • N- and C-terminal sequencing
  • Murine isotype determination
Why combine methods? No single assay answers every characterization question. Combining immunoassay, electrophoresis, chromatography, and mass-based analysis can provide a more complete picture of antibody quantity, identity, and performance.

Typical Project Workflow

1. Scope Review

Define whether the goal is titer screening, purity review, identity confirmation, isotyping, or a broader characterization package.

2. Method Selection

Select one or more methods based on sample type, expected output, and whether results will support assay transfer or downstream development.

3. Analytical Testing

Perform the selected characterization workflow and generate data relevant to the project objective.

4. Reporting

Provide results, supporting observations, and applicable images or method outputs for review and decision-making.

Sample Requirements

Service Area Typical Input Purpose
Western Blot Assay Purified protein: 15–20 µg Evaluate immunoreactivity and expected banding profile
Western Blot Assay Cellular lysate containing target protein: 500 µg Assess target detection in more complex biological material
ELISA Testing Project-specific Titration, quantitation, and screening of antibody samples
Murine Isotyping Supernatant or ascites Determine murine antibody isotype
Advanced Analytical Testing Project-specific purified antibody material Support HPLC, MS, IEF, and sequencing-based characterization

Final sample requirements depend on the selected method, antibody format, and analytical objective.

Typical Deliverables

Project Data Package

  • Assay or analytical results aligned to project scope
  • Method-specific observations and interpretation support
  • Transferable ELISA protocol when applicable

Supporting Outputs

  • Western blot image or gel photo when applicable
  • Purity and identity review from selected orthogonal methods
  • Documentation supporting downstream decision-making

FAQ

What does antibody characterization include?
Depending on project scope, characterization may include ELISA titration and quantitation, Western blot, HPLC, SDS-PAGE, IEF, intact mass determination, terminal sequencing, and murine isotype testing.
Can you develop and transfer an ELISA method?
Yes. Once an ELISA has been developed, the protocol can be transferred and then used to test your antibody samples.
What sample quantity is needed for Western blot testing?
Typical inputs are 15–20 µg of purified protein or about 500 µg of cellular lysate containing the protein of interest.
Do you support both monoclonal and polyclonal antibodies?
Yes. The service is positioned for characterization of both monoclonal and polyclonal antibody samples.
Can murine antibodies be isotyped?
Yes. Murine isotype determination can be performed for antibodies in supernatants or ascites.
Can methods be combined in one project?
Yes. Projects can be designed around one specific assay or a broader multi-method characterization workflow.
More FAQ'sAll FAQ's

Contact & Quote Request

For the fastest quote, include antibody type, sample format, available quantity, desired characterization method(s), target protein information, and whether you need a single assay or a broader multi-method package.

Helpful details to include

  • Monoclonal or polyclonal antibody
  • Sample source and available amount
  • Need for ELISA, Western blot, MS, sequencing, or isotyping
  • Target application and desired turnaround context

Fastest path

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Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
Thank you for your understanding.

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