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Dextran Modification and Conjugation
Dextran Modification and Conjugation

Dextran Conjugates

Bio-Synthesis provides high quality modification and activation of dextran to protein or other molecules using flexible conjugation chemistries, which cover almost every aspect you may encounter during the preparation phase in diagnostic and drug discovery research. These natural occurring hydrophilic polysaccharides are synthesized by Leu-conostoc bacteria. Dextrans are characterized by their high molecular weight, good water solubility, low toxicity, and relative inertness. These properties make the modification of a biomolecule with dextran an effective water-soluble carrier for drugs in vivo, as a hapten carrier to illicit an immune response, as a multifunctional cross linker, and as a stabilizer of enzymes and other proteins. Moreover, their biologically uncommon α-1,6-polyglucose linkages are resistant to cleavage by most endogenouscellular glycosidases. Therefore, dextran conjugates make ideal long-term tracers for live cells. Fluorescent dextrans also serve as valuable markers for cell loading of macromolecules by microinjection, vesicular fusion, electroporation, as well as for the uptake and internal processing of exogenous materials by phagocytotic and endocytotic pathways.

Dextran Modification Service Portfolios

  • Peptide-dextran modification
  • Protein-dextran modification
  • Fab'-dextran modification
  • Dextran-anti-IgD conjugates
  • siRNA-dextran conjugation
  • Antisense oligo-dextran conjugation
  • Enzyme-dextran modification
  • Fluorescent dye-dextran conjugates
  • Small molecule-dextran modification
  • Dextran-drug conjugates
  • Dextran-metal chelator (DTPA) conjugates

All bioconjugation projects are meticulously monitored according to Bio-Synthesis's stringent quality assurance and quality control standards, which are fully backed up by a bioanalytical laboratory.

What is a Dextran Polymer?

Dextrans are polysaccharides consisting of repeating units of D-glucose linked together in glycosidic bonds with molecular weights ≥1000 dalton. The hydroxylic content of the dextran sugar backbone makes the polymer very hydrophilic and can be easily modified for attaching to other molecules. The hydroxyl functional groups of dextran are present on each monomer in the chain. Each monomer contains of at least 3 hydroxyls (4 on the terminal units) that may undergo derivatization reactions. This multivalent nature of dextran allows molecules to be attached at numerous sites along the polymer chain.

Bio-Synthesis offers dextran modification and conjugation services using various molecular weight sizes ranging from 3,000 to 2,000,000 daltons. Because unlabeled dextrans are polydispersed and may become more so during the chemical processes required for their modification and purification, the actual molecular weights present in a particular sample may have a broad distribution. For example, our 3,000 MW dextran preparations contain polymers with molecular weights predominantly in the range of ~1,5003,000 daltons, including the dye or other labels, while our 70,000 MW dextran preparations contain polymers with molecular weights ranging from 60,000 to 90,000 daltons.

Contact our Technical Service Center at 800.220.0627 or contact us online with your detailed project specifications. A project manager will be assigned to help you with the design and development of an appropriate synthetic method for your specific needs.

Sample Submission Requirement:

Biomolecules supplied by customers should be sufficiently pure. Please provide up to 3 mgs of starting material with the necessary data for purity assessment. Commercial available biopolymers can be supplied by customers or synthesize and ordered through Bio-Synthesis.

Price

The price varies based on the project specifications. Our service includes materials and labor for conjugation only! Price does not include the cost of biopolymer synthesis or ordering through Bio-Synthesis from commercial vendors and, if deemed necessary, biopolymer modification to introduce additional functional groups, extra linkers, spacers, etc. Please contact us for a quote.

Service Descriptions

Chemistry:

Reaction of aldehyde with amine, hydroxyl amine or hydrazine containing compounds.

Service Specification:

After standard desalting or purification, a small percent of heterogeneous products containing single or multi-site conjugate per molecule may exist.

Procedure:

After labeling, final conjugates must first be isolated from excess or unreacted reagent by gel filtration or dialysis. In many cases, simple dialysis may suffice to remove unreacted reagent from the reaction solution. Additional purification techniques such as stirred cell filtration, tangential flow filtration (TFF), and gel filtration chromatography may also be used to either remove excess reagent or isolate and characterized the cross-linked product. With exception of dialysis, additional purification techniques are usually recommended if the protein/antibody is significantly larger (>3-fold) than the modifying or coupling reagent. For reagents (mostly protein and other biological molecules) that are similar in size or larger than the antibody, one must resort to other purification techniques such as affinity chromatography, ion-exchange chromatography, and hydrophobic interaction chromatography.

Cross-linked target molecules may then be further characterized by gel electrophoresis. It may be subject to additional analyses with an additional fee. This includes spectroscopic (MALDI-TOF, ESI, LC-MS Fluorescence), electrophoresis, immunochemical biochemical, enzymatical analysis, and TLC. QC (quality control) and QA (quality assurance) procedures are also followed independently to offer you double guarantee for the highest quality possible. Moreover, our dedicated technical account managers will guide you through every step of the process and constantly keep you informed of the latest project progress.

Ordering and Submitting Requests for Bioconjugation Services

For us to better understand your customized project, please complete our Bioconjugation Service Questionnaire. The more our chemists understand your project needs, the more accurate feedback we will be able to provide you.  Provide us with your project details will enable to us to recommend the best reagents to use for your project.  The most useful and readily available tools for bioconjugation projects are cross-linking reagents. A large number of cross-linkers, also known as bifunctional reagents, have been developed.  There are several ways to classify the cross-linkers, such as the type of reactive group, hydrophobicity or hydrophilicity, and the length of the spacer between reactive groups.  Other factors to consider are whether the two reactive groups are the same or different (for example, heterobifunctional or homobifunctional reagents), whether the spacer is cleavable, and whether the reagents are membrane permeable or impermeable.  The most accessible and abundant reactive groups in proteins are the ϵ-amino groups of lysine.  Therefore, a large number of the most common cross-linkers are amino selective reagents, such as imidoesters, sulfo-N-hydroxysuccinimide esters, and N-hydroxysuccinimide esters.  Due to the high reactivity of the thiol group with N-ethylmaleimide, iodoacetate and a-halocarbonyl compounds, new cross-linkers have been developed that contain maleimide and a-carbonyl moieties.  Usually, N-alkylmaleimides aremore stable than their N-aryl counterparts.

In addition to the reactive groups on the cross-linkers, a wide variety of connectors and spacer arms have also been developed.  The nature and length of the spacer arm play an important role in the functionality.  Longer spacer arms are generally more effective when coupling large proteins or those with sterically protected reactive side-chains.  Other important considerations are the hydrophobicity, hydrophilicity, and the conformational flexibility.  Long aliphatic chains generally fold on themselves when in an aqueous environment, which makes the actual distance spanned by such linker arms less than expected.  Instead, spacers that contain more rigid structures (for example, aromatic groups or cycloalkanes) should be used.  These structures, however, tend to be very hydrophobic which could significantly decrease the solubility of the modified molecules or even modify some of their properties.  In such cases, it is recommended to choose a spacer that contains an alkylether (PEO) chain.  Bio-Synthesis offers several cross-linkers with PEO chains, such as thiol-binding homobifunctional reagents, heterobifunctional based, and their derivatives.

Within 3-5 days upon receiving your project scope, we will provide you an appropriate quotation. An order can be placed with PO (Purchase Order) or major credit cards ( ). Your credit card will be billed under Bio-Synthesis, Inc.