Bridged Nucleic Acids (BNA)
Scorpion Primers is a highly sensitive, sequence specific dual labeled hairpin, uni-probe that combines a target specific PCR primer and a target specific DNA probing sequence in a single molecule design. This uni-molecular mechanism results in faster kinetic reaction and lead to instantaneious generation of a fluorescent signal in real-time qPCR without incur any competiting side reactions. These primers doe not require enzymatic cleavage of the probe duing PCR cycling. The unique properties of Scorpions Primers enabled reliable probe design with stronger signals, shorter reaction times and better discrimination power than other bi-molecular system. Scorpions probes offer valuable tools for rapid, real-time PCR, endpoint PCR, SNP detection, and gene quantification.
All Bio-Synthesis's Scorpion Primers are available at a fixed price per oligonucleotide, within the following parameters:
If any help is needed qPCR primer design for Scorpions Primers/Probes or the combination of dyes, please do not hesitate to contact our scientific support that will be happy to help you.
Learn more about Bridged Nucleic Acids (BNAs).
All scorpion primers includes DNA probing sequence held in a stem-loop conformation with a 5' reporter dye and an internal quencher dye directly linked to the 5' end of a PCR primer via a hexathylene glycol blocker. These probes are HPLC purified and controlled by MALDI-TOF Mass Spectrometry and deliver in lyophilized format.
Please browse our selection below to find a suitable dye and quencher combination, or you may contact us
Guarantee Delivered Quantity (nmol)*
* including dual HPLC and Mass Check
For probe design, larger molar amounts, large volume orders or other special requests, please contact us at email@example.com
Uni-Probe: 5' Flurophore
* Dyes indicated in parentheses are reporter groups that quench with less than optimal efficiency but can still be used in probes having hairpin design.
*Estimate is based on 2 nmoles or 32 µg for 1 OD and 200 nM in 25 µl reaction (5.0 pmol/reaction). Estimate is based on an average sequence length of 50 bases (uni-probe).
In order to ensure that there is no background fluorescence, 100% of the dual labeled probes are purified by single or dual rp-HPLC and ie-HPLC as a standard protocol for all dual–labeled probes which give 90-97% purity. Depending on the type of the probes, one or two purification may performed to ensure the highest purity level
All dual labeled probes are quality checked by MALDI-TOP Mass spectrometry , analytical HPLC and analyze by Fluorometric Scan (ABS/EM) . In addition, the signal-to-background ratio is measured against a target sequence for all molecular beacons.
3-5 Working days
-20 oC to -70 oC
Oligonucleotides are stable in solution at 4oC for up to 2 weeks. Properly reconstituted material stored at -20oC should be stable for at least 6 months. Lyophilized DNA (when kept at -20oC) in a nuclease-free environment should be stable for year
Every oligo is quality checked by MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE).
Fully deprotected and desalted. Purified by PAGE or RP-HPLC
Delivered as dried-down product in opaque tubes
Turn-around time is dependent upon successful QC validation and does not include shipping time.
See information on our oligos: storage recommendations
Shipped by mail or express delivery, dry, in individual, opaque tubes
Oligonucleotides are delivered with an Oligonucleotide Technical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, length, extinction coefficient and purification data.
Additional services may increase turn-around time
Please contact your local Bio-Synthesis representative
On-line (contact us), by email (firstname.lastname@example.org) or fax