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Dual-labeled probe also known as hydrolysis probes are the most common type of qPCR probes. These are linear single stranded oligonucleotide with a fluorescent reporter dye at 5' end and a quencher at 3' end of an oligonucleotide typically 20 to 30 nucleotide in length. The quencher molecule inhibits the natural fluorescence emission of the reporter dye by Forster-type energy transfer.

Bio-Synthesis is a supplier of dual labeled oligonucleotide probe with arrays of dye and quenchers combinations. All qPCR probes are available at a fixed price per oligonucleotide, within the following parameters:

  • DNA, BNA chimeric mix base up 35 bases in length
  • Wobbles (non-defined ratio) possible
  • HPLC purified in 10 nmole, 25 nmole up to 1000 nmole
  • QC by MALDI, analytical HPLC and Fluoroscan
  • Deliver lyophilized in tube
  • TAT of 3 working days

If any help is needed qPCR primer design or the combination of dyes that should be used on a specific thermocycler, please do not hesitate to contact our scientific support that will be happy to help you.

Custom Dual Labeled Probes and Primers

Bio-Synthesis has developed a throughput platform to manufacture high quality real-Time qPCR dual labelled probles with >95% guaranteed purity which may be used in the following applications:

  • Non-Isotopic, Real Time PCR
  • Gene expression profiling
  • Single nucleotide polymorphisms (SNPs)
  • Pathogen and mutation detection
  • Fluorescence in situ hybridization (FISH)
  • Viral identification and quantification
  • Gene copy determination
  • Microarray validation

The optimal recommended length of Double-Dye Oligonucleotide probes is 25 bases. While the maximum length of Double-Dye Oligonucleotides is 35 bases, it is unlikely to have efficient quenching with an end-labeled probe with sequences longer than 30 bases. In the case of extra long Double-Dye Oligonucleotides probes are required, we recommend to consider positioning the quencher internally. Conversely, sequence shorter than 20 bases may have adding BNA to your probe for:

Benefit of Adding BNA to Your qPCR SNP Probe

  • Increased thermal stability and hybridization specificity
  • Greater accuracy in SNP detection, allele discrimination, and in vitro quantification or detection
  • Easier and more sensitive probe designs for problematic target sequences

Contact Bio-Synthesis for qPCR primer design or visit our technical educational warehouse and search for article regarding real-time qPCR primers and probes.

Purification

In order to ensure that there is no background fluorescence, 100% of the dual labeled probes are purified by single or dual rp-HPLC and ie-HPLC as a standard protocol for all dual–labeled probes which give 90-97% purity. Depending on the type of the probes, one or two purification may performed to ensure the highest purity level

Quality Control

All dual labeled probes are quality checked by MALDI-TOP Mass spectrometry , analytical HPLC and analyze by Fluorometric Scan (ABS/EM) . In addition, the signal-to-background ratio is measured against a target sequence for all molecular beacons.

Pricing

All dual–labeled fluorogenic probes and Molecular Beacons includes the 5'–reporter dye, 3'–quencher, and HPLC purification. Please submit your order or quotation request to the client relations department at info@biosyn.com, fax (972) 420-0442, or you can use our online ordering system.

Browse our selection using the tabs below. If you can't find the dye and quencher combination listed below, please contact us

3' Black Hole Quencher Dual Labeled Probes

The optimal length of Double-Dye Oligonucleotide probes with a 3’ BHQ dark quencher is 25 bases. In case extra long Double-Dye Oligonucleotides probes are required, this BHQ1 and BHQ-2 can be coupled internally.

3' BHQ Scale Minimum Guarantee Yield USD
5’ TAMRA + 3 ‘BHQ-2
100 nmole scale 8-10 nmole dual HPLC Purified $350.00
250 nmole scale 20-25 nmole dual HPLC Purified $420.00
1.0 umole scale 55-60 nmole dual HPLC Purified $600.00
5’ FAM + 3’ BHQ-1
100 nmole scale 8-10 nmole dual HPLC Purified $150.00
250 nmole scale 20-25 nmole dual HPLC Purified $250.00
1.0 umole scale 55-60 nmole dual HPLC Purified $365.00
5' FAM + Internal T-BHQ-1 / 3' C3 250 nmole 10-15 nmole HPLC purified $295.00
1 umole scale 30-35 nmole HPLC purified $515.00
5’ HEX + 3’ BHQ-1
100 nmole scale 8-10 nmole dual HPLC Purified $235.00
250 nmole scale 20-25 nmole dual HPLC Purified $335.00
1.0 umole scale 55-60 nmole dual HPLC Purified $475.00
5’ TET + 3’ BHQ-1
100 nmole scale 8-10 nmole dual HPLC Purified $235.00
250 nmole scale 20-25 nmole dual HPLC Purified $335.00
1.0 umole scale 55-60 nmole dual HPLC Purified $475.00
5’ JOE + 3’ BHQ-1 100 nmole scale 2-3 nmole dual HPLC purified $300.00
250 nmole scale 8-10 nmole dual HPLC purified $450.00
1.0 umole scale 35-40 nmole dual HPLC Purified $600.00
5’ ROX + 3’ BHQ-2 100 nmole scale 2-3 nmole dual HPLC purified $300.00
250 nmole scale 8-10 nmole dual HPLC purified $420.00
1.0 umole scale 25-30 nmole dual HPLC Purified $625.00
5’ Rhodamine-6G + 3’ BHQ-1 100 nmole scale 2-3 nmole dual HPLC purified $300.00
250 nmole scale 8-10 nmole dual HPLC purified $370.00
1.0 umole scale 25-30 nmole dual HPLC Purified $550.00
5’ Texas Red® + 3’ BHQ-2 100 nmole scale 2-3 nmole dual HPLC purified $375.00
250 nmole scale 8-10 nmole dual HPLC purified $415.00
1.0 umole scale 35-40 nmole dual HPLC Purified $600.00
5’ Yakima Yellow® + 3’ BHQ-1 100 nmole scale 5 nmole HPLC Purified $345.00
250 nmole scale 10-15 nmole HPLC Purified $425.00
1.0 umole scale 45-50 nmole HPLC Purified $600.00
5’ Dragonfly Orang + 3’ BHQ-2 100 nmole scale 2-3 nmole dual HPLC purified $345.00
250 nmole scale 8-10 nmole dual HPLC purified $425.00
1.0 umole scale 25-30 nmole dual HPLC Purified $600.00
5’ Cy® 3 + 3’ BHQ-2 100 nmole scale 5 nmole HPLC Purified $295.00
250 nmole scale 10-15 nmole HPLC Purified $372.00
1.0 umole scale 45-50 nmole HPLC Purified $551.00
5’ Cy5® + 3’ BHQ-2 100 nmole scale 5 nmole HPLC Purified $295.00
250 nmole scale 10-15 nmole HPLC Purified $372.00
1.0 umole scale 45-50 nmole HPLC Purified $551.00
5’ Cy5® + 3’ BHQ-3 100 nmole scale 5 nmole HPLC Purified $650.00
250 nmole scale 10-15 nmole HPLC Purified $850.00
1.0 umole scale 45-50 nmole HPLC Purified $1,100.00
5’ DYOMICS + 3’ BHQ 100 nmole scale 2-3 nmole dual HPLC purified Inquire
250 nmole scale 8-10 nmole dual HPLC purified Inquire
1.0 umole scale 25-30 nmole dual HPLC Purified Inquire
5’ ATTO + 3’ BHQ 100 nmole scale 2-3 nmole dual HPLC purified $345.00
250 nmole scale 8-10 nmole dual HPLC purified $420.00
1.0 umole scale 25-30 nmole dual HPLC Purified $600.00
5’ Alexa + 3’ BHQ 100 nmole scale 2-3 nmole dual HPLC purified $345.00
250 nmole scale 8-10 nmole dual HPLC purified $420.00
1.0 umole scale 25-30 nmole dual HPLC Purified $600.00

3' TAMRA Dual Labeled Probes

TAMRA has an absorbance maximum of 565 nm and an emission maximum of 580 nm. In FRET application, a common donor-acceptor pair is 6-FAM (donor) / TAMRA (acceptor), due to their good spectral overlap. As the donor, 6-FAM is excited at 492 nm and transfers this energy to TAMRA, which then emits light at 580 nm. This FRET oligo probe design are widely used to in vivo studies to monitor biochemical reactions 1 .

TAMRA also can be used as a FRET-based quencher moiety in real-time PCR probes such as TaqMan probes2 , Scorpion primers3 and Molecular Beacons 4 . For such probes, 6-FAM is used as the reporter moiety, and its emission at 521 nm is monitored. When 6-FAM and TAMRA are in close proximity, the former’s fluorescence at 521 nm is quenched by the latter. After sufficient spatial separation of the two dyes during the course of the assay, 6-FAM’s fluorescence is no longer quenched, and its fluorescence signal becomes observable.

TAMRA also can be used as a FRET-based quencher moiety in real-time PCR probes such as TaqMan probes2 , Scorpion primers3 and Molecular Beacons4 . For such probes, 6-FAM is used as the reporter moiety, and its emission at 521 nm is monitored. When 6-FAM and TAMRA are in close proximity, the former’s fluorescence at 521 nm is quenched by the latter. After sufficient spatial separation of the two dyes during the course of the assay, 6-FAM’s fluorescence is no longer quenched, and its fluorescence signal becomes observable.

TAMRA also can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos labeled with TAMRA at the 5’-end can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products.

We offer 3'-TAMRA CPG for 3' labeling and TAMRA-dT for labelling within the sequence. We also offer TAMRA NHS ester for labelling amino-modified oligonucleotides post-synthetically. All Double-Dye Oligonucleotides are single ie-HPLC or dual rp-HPLC and ie-HPLC purified. The length of Double-Dyes probes should be comprised between 15 and 35 bases. The optimal recommended length of Double-Dye Oligonucleotides with a 3’ TAMRA is 25 bases.

3' TAMRA Scale Minimum Guarantee Yield USD
5’ FAM + 3' TAMRA 50 nmole scale 5 nmole dual HPLC purified $180.00
100 nmole scale 8-10 nmole dual HPLC Purified $220.00
250 nmole scale 20-25 nmole dual HPLC Purified $280.00
1.0 umole scale 55-60 nmole dual HPLC Purified $365.00
5' TET + 3' TAMRA 50 nmole scale 5 nmole HPLC purified $220.00
100 nmole scale 8-10 nmole dual HPLC Purified $275.00
250 nmole scale 20-25 nmole dual HPLC Purified $310.00
1.0 umole scale 55-60 nmole dual HPLC Purified $475.00
5' Yakima Yellow + 3' TAMRA 100 nmole scale 8-10 nmole HPLC Purified $330.00
250 nmole scale 25-30 nmole HPLC Purified $380.00
1.0 umole scale 55-60 nmole dual HPLC Purified $570.00
5' HEX + 3' TAMRA 100 nmole scale 8-10 nmole HPLC Purified $275.00
250 nmole scale 20-25 nmole dual HPLC Purified $375.00
1.0 umole scale 55-60 nmole dual HPLC Purified $495.00

Carboxytetramethylrhodamine (TAMRA) is a fluorescent dye that is a derivative of rhodamine, and is used to label oligonucleotides at the 5’- or 3’-ends, or internally. TAMRA has an absorbance maximum of 565 nm and an emission maximum of 580 nm. TAMRA-modified oligonucleotides play a particularly important role in both fluorescence resonance energy transfer (FRET) and real-time PCR applications.

FRET is a distance-dependent interaction between two dye molecules in which excitation is radiationlessly transferred from one dye (the donor) to the second dye (the acceptor), due to spectral overlap. Because the efficiency of the energy transfer is extremely sensitive to the distance between the molecules (varying as the inverse sixth power of that distance) (1), FRET can be used to study biological phenomena that produce changes in molecular proximity (2). For oligonucleotides slated for use in FRET application, a common donor-acceptor pair is 6-FAM (donor) / TAMRA (acceptor), due to their good spectral overlap. As the donor, 6-FAM is excited at 492 nm and transfers this energy to TAMRA, which then emits light at 580 nm. FRET oligo probes are widely used to monitor biochemical reactions, particularly in in vivo studies (3).

Besides being used as a FRET fluorophore, TAMRA also can be used as a FRET-based quencher moiety in real-time PCR probes such as TaqMan probes (4), Scorpion primers (5) and Molecular Beacons (6). For such probes, 6-FAM is used as the reporter moiety, and its emission at 521 nm is monitored. When 6-FAM and TAMRA are in close proximity, the former’s fluorescence at 521 nm is quenched by the latter. After sufficient spatial separation of the two dyes during the course of the assay, 6-FAM’s fluorescence is no longer quenched, and its fluorescence signal becomes observable.

TAMRA also can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos labeled with TAMRA at the 5’-end can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatelite) products.

Note that, because TAMRA is in the form of an NHS ester, the oligo first must be synthesized with an Amino C6 Linker (for the ends) or the Amino C6 version of the base phosphoramidite (for internal labeling). The TAMRA-NHS ester is then manually attached to the oligo through the amino group in a separate reaction post-synthesis.

References:

  1. Didenko, V.V. DNA Probes Using Fluorescence Resonance Energy Transfer (FRET): Designs and Applications. Biotechniques (2001), 31 : 1106-1121.
  2. Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W., Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl. (1995), 4 : 1-6.
  3. Thelwell, N., Millington, S., Solinas, A., Booth, J., Brown, T. Mode of action and application of Scorpion primers to mutation detection. Nucleic Acids Res. (2000), 28: 3752-3761
  4. Tyagi, S., Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. (1996), 14: 303-308.

3' Eclipse Dark Quencher Dual Labeled Probes

The Eclipse quencher developed by Epoch, is a highly stable molecule and can be used safely in all common oligo deprotection schemes. The absorbance maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl. In addition, the structure of the Eclipse Quencher is substantially more electron deficient than that of dabcyl and this leads to better quenching over a wider range of dyes, especially those with emission maxima at longer wavelengths (red shifted) such as Redmond Red and Cy5. In addition, with an absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes.

The optimal length of Double-Dye Oligonucleotide probes with a 3’ Eclipse® Dark Quencher is 25 bases.

3' Eclipse Dark Quencher Scale Price
5' FAM + 3' Eclipse DQ 100 nmole 5 nmole HPLC Purified $256.00
250 nmole 10-15 nmole HPLC Purified $314.00
1.0 umole 45-50 nmole HPLC Purified $519.00
5' HEX + 3' Eclipse DQ 100 nmole 5 nmole HPLC Purified $256.00
250 nmole 10-15 nmole HPLC Purified $314.00
1.0 umole 45-50 nmole HPLC Purified $519.00
5' TET + 3' Eclipse DQ 100 nmole 5 nmole HPLC Purified $256.00
250 nmole 10-15 nmole HPLC Purified $314.00
1.0 umole 45-50 nmole HPLC Purified $519.00
5' Cy5 + 3' Eclipse DQ 100 nmole 5 nmole HPLC Purified $650.00
250 nmole 10-15 nmole HPLC Purified $850.00
1.0 umole 45-50 nmole HPLC Purified $1,100.00
5' Redmond Red 100 nmole 5 nmole HPLC Purified $370.00
250 nmole 10-15 nmole HPLC Purified $430.00
1.0 umole 45-50 nmole HPLC Purified $600.00
5' JOE + 3' Eclipse DQ 100 nmole 5 nmole HPLC Purified $321.00
250 nmole 10-15 nmole HPLC Purified $378.00
1.0 umole 45-50 nmole HPLC Purified $551.00
5' Rhodamine-6G + 3' Eclipse DQ 100 nmole 2-3 nmole dual HPLC purified $321.00
250 nmole 8-10 nmole dual HPLC purified $378.00
1.0 umole 25-30 nmole dual HPLC Purified $551.00
5' Yakima Yellow® + 3' Eclipse DQ 100 nmole 5 nmole HPLC Purified $370.00
250 nmole 10-15 nmole HPLC Purified $430.00
1.0 umole 45-50 nmole HPLC Purified $600.00
5' DYOMICS + 3' Eclipse DQ 100 nmole 2-3 nmole dual HPLC purified On Request
250 nmole 8-10 nmole dual HPLC purified On Request
1.0 umole 25-30 nmole dual HPLC Purified On Request
5' ATTO + 3' Eclipse DQ 100 nmole 2-3 nmole dual HPLC purified $350.00
250 nmole 8-10 nmole dual HPLC purified $445.00
1.0 umole 25-30 nmole dual HPLC Purified $590.00

3' IQ Quencher Dual Labeled Probes

The Instant Quencher IQ4, IQ2 are highly stable molecule and can be used safely in all common oligo deprotection schemes. IQ Quencher is capable of effective performance in a wide range of colored FRET probes from FAM (EX 495 nm) to Cy5 (Ex 650 nm).

The optimal length of Double-Dye Oligonucleotide probes with a 3’ IQ4 dark quencher is 25 bases. In case extra long Double-Dye Oligonucleotides probes are required, this IQ4 can be coupled internally

3' Instant Quencher IQ Scale Price
5' FAM + IQ2, IQ4 100 nmole 15 nmole HPLC Purified $195.00
250 nmole 25 nmole HPLC Purified $275.00
1.0 umole 50 nmole HPLC Purified $415.00
5' HEX + 3' IQ2 or IQ4 100 nmole 15 nmole HPLC Purified $245.00
250 nmole 25 nmole HPLC Purified $350.00
1.0 umole 50 nmole HPLC Purified $520.00
5' TET + 3' IQ2 or IQ4 100 nmole 15 nmole HPLC Purified $245.00
250 nmole 25 nmole HPLC Purified $350.00
1.0 umole 50 nmole HPLC Purified $520.00
5' JOE + 3' IQ2 or IQ4 100 nmole 15 nmole HPLC Purified $245.00
250 nmole 25 nmole HPLC Purified $350.00
1.0 umole 50 nmole HPLC Purified $520.00
5' Cy3 + 3' IQ4 100 nmole 2 nmole HPLC Purified $225.00
250 nmole 10 nmole HPLC Purified $260.00
1.0 umole 20 nmole HPLC Purified $475.00
5' CY5 + 3' IQ4 100 nmole 2 nmole HPLC Purified $225.00
250 nmole 10 nmole HPLC Purified $260.00
1.0 umole 20 nmole HPLC Purified $475.00
5' TAMRA + 3' IQ4 100 nmole NA NA
250 nmole 10 nmole HPLC Purified $345.00
1.0 umole 20 nmole HPLC Purified $515.00
5' ROX+ 3' IQ4 100 nmole NA NA
250 nmole 10 nmole HPLC Purified $420.00
1.0 umole 20 nmole HPLC Purified $630.00
5' Texas Red + 3' IQ4 100 nmole NA NA
250 nmole 10 nmole HPLC Purified $420.00
1.0 umole 20 nmole HPLC Purified $630.00
5’ ATTO + 3’ IQ4 100 nmole scale 2-3 nmole dual HPLC purified $345.00
250 nmole scale 8-10 nmole dual HPLC purified $420.00
1.0 umole scale 25-30 nmole dual HPLC Purified $600.00
5’ Alexa + 3’ IQ4 100 nmole scale 2-3 nmole dual HPLC purified $345.00
250 nmole scale 8-10 nmole dual HPLC purified $420.00
1.0 umole scale 25-30 nmole dual HPLC Purified $600.00

3' Blackberry Quencher Dual Labeled Probes and Others

Blackberry quencher BBQ650 can be use to replace BHQ3. BBQ650 dark quencher has broad absorbance spectra ranging from 550-750 with peak absorbance at ~650 nmm. This quencher is ideal for use with Texas Red®, Cy5TM, TAMRA, Redmond Red, DyeLight and other fluorescent dyes that emit in the red spectral range.

The optimal length of Double-Dye Oligonucleotide probes with a 3’ BBQ650 dark quencher is 25 bases. In case extra long Double-Dye Oligonucleotides probes are required, this BBQ650 can be coupled internally.

3' Blackberry® Quencher (BBQ 650®) Scale Price
5' JOE + 3' IQ2 or IQ4 100 nmole 15 nmole HPLC Purified $245.00
250 nmole 25 nmole HPLC Purified $350.00
1.0 umole 50 nmole HPLC Purified $520.00
5' Cy3 + 3' BBQ650 100 nmole 2 nmole HPLC Purified $225.00
250 nmole 10 nmole HPLC Purified $260.00
1.0 umole 20 nmole HPLC Purified $475.00
5' CY5 + 3' BBQ650 100 nmole 2 nmole HPLC Purified $225.00
250 nmole 10 nmole HPLC Purified $260.00
1.0 umole 20 nmole HPLC Purified $475.00
5' TAMRA + 3' BBQ650 100 nmole NA NA
250 nmole 10 nmole HPLC Purified $345.00
1.0 umole 20 nmole HPLC Purified $515.00
5' ROX+ 3' BBQ650 100 nmole NA NA
250 nmole 10 nmole HPLC Purified $420.00
1.0 umole 20 nmole HPLC Purified $630.00
5' Texas Red + 3' BBQ650 100 nmole NA NA
250 nmole 10 nmole HPLC Purified $420.00
1.0 umole 20 nmole HPLC Purified $630.00
5’ ATTO + 3’ BBQ650 100 nmole scale 2-3 nmole dual HPLC purified $345.00
250 nmole scale 8-10 nmole dual HPLC purified $420.00
1.0 umole scale 25-30 nmole dual HPLC Purified $600.00
5’ Alexa + 3’ BBQ650 100 nmole scale 2-3 nmole dual HPLC purified $345.00
250 nmole scale 8-10 nmole dual HPLC purified $420.00
1.0 umole scale 25-30 nmole dual HPLC Purified $600.00

Delivery times

3-5 Working days

Packaging

Lyophilised

Shipping conditions

Room temperature

Storage conditions

-20 °C to -70 °C

Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Lyophilized DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

Specifications

Quality control:

Every oligo is quality checked by MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE).

Purification

Fully deprotected and desalted. Purified by PAGE or RP-HPLC

Length

Length
LightCycler probes 15 to 40 mers (optimal length: 20 to 30 mers)
Dual-labeled fluorogenic probes: 15 to 40 mers
Molecular Beacons 15 to 40 mers
Scorpions probes 30 to 60 mers (uni-probe)
15 to 45 mers (bi-probe)
Plexor up to 30 mers

Backbone

Phosphodiester bond

Format

Delivered as dried-down product in opaque tubes

Turn-around time:

Turn-around time
LightCycler probes 4 to 5 working days
Dual-labeled fluorogenic probes: 5 to 6 working days
Molecular Beacons 5 to 6 working days
Scorpions probes 7 to 10 working days

Turn-around time is dependent upon successful QC validation and does not include shipping time.

Storage and stability:

See information on our oligos: storage recommendations

Shipment:

Shipped by mail or express delivery, dry, in individual, opaque tubes

Technical Documentation:

Oligonucleotides are delivered with an OligonucleotideTechnical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, length, extinction coefficient and purification data.

Additional Services:

  • Probe design services
  • High-throughput screening formats (microplates, etc)
  • Aliquoting

Additional services may increase turn-around time

Pricing:

Please contact your local Bio-Synthesis representative

Ordering:

On-line (contact us), by email (info@biosyn.com) or fax

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please call 1-972-420-8505 or Fax at 1-972-420-0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."