Custom AuNP bioconjugation for oligonucleotides, antibodies, proteins, peptides, aptamers, small molecules, and diagnostic assay development using optimized nanoparticle size, surface chemistry, purification, and characterization.
Gold nanoparticle conjugation is a nanomaterial-based bioconjugation approach used to attach biomolecules and functional ligands to nanoscale gold surfaces. Gold nanoparticles are valued for their strong colorimetric signal, surface plasmon resonance, high surface-to-volume ratio, and compatibility with multiple surface chemistries.
Bio-Synthesis provides custom gold nanoparticle conjugation services for research, diagnostic, biosensor, labeling, and lateral flow assay applications. We help select the appropriate AuNP size, linker chemistry, biomolecule format, purification strategy, and characterization workflow based on your intended use.
AuNPs provide intense visible color and plasmon-based signal for colorimetric assays and detection systems.
Thiol-gold, passive adsorption, EDC/NHS, maleimide, biotin-streptavidin, and PEGylated surfaces can be used.
Particle diameter can be selected to balance loading capacity, signal intensity, stability, and assay performance.
Useful for lateral flow assays, biosensors, molecular diagnostics, imaging, and probe development.
Gold nanoparticles can be functionalized with oligonucleotides, antibodies, proteins, peptides, aptamers, PEG chains, dyes, small molecules, and targeting ligands. Surface design controls stability, spacing, biological activity, and detection performance.
Biomolecules can be coupled through thiol-gold binding, covalent linkers, adsorption, affinity systems, or functionalized coatings.
Buffer, salt, pH, ligand density, PEG spacing, and purification are optimized to reduce aggregation and preserve activity.
Particle size and surface loading influence visual signal, kinetics, nonspecific binding, and detection sensitivity.
DNA, RNA, thiol-modified oligos, amine-modified oligos, aptamers, probes, and modified nucleic acids.
Explore oligo-gold conjugation →
Monoclonal antibodies, polyclonal antibodies, Fab, scFv, nanobodies, and capture or detection antibodies.
Explore antibody-gold conjugation →
Recombinant proteins, enzymes, carrier proteins, streptavidin, avidin, and protein biomarkers.
Explore protein-gold conjugation →
Thiolated peptides, cysteine-containing peptides, targeting peptides, cell-penetrating peptides, and peptide probes.
Explore peptide-gold conjugation →
Biotin, haptens, drugs, dyes, fluorophores, chelators, affinity tags, and targeting ligands.
Explore ligand-gold conjugation →
PEGylation, spacer arms, charged coatings, carboxyl, amine, maleimide, and mixed monolayer designs.
Explore PEGylated AuNPs →
Direct attachment of thiol-modified oligonucleotides, peptides, PEG, or ligands through strong Au-S surface interactions.
Common for antibodies and proteins using optimized pH, ionic strength, and stabilizers to preserve binding activity.
Carboxyl-functionalized AuNPs can be activated for amide bond formation with amine-containing biomolecules.
Maleimide-functionalized particles enable selective coupling to cysteine-containing peptides, proteins, or antibody fragments.
Biotinylated biomolecules can be immobilized using streptavidin- or avidin-functionalized AuNP platforms.
PEG spacers and passivating ligands can improve colloidal stability, reduce nonspecific binding, and control ligand spacing.
Particle size affects optical signal, surface area, biomolecule loading, steric accessibility, colloidal stability, and assay sensitivity. The guide below provides practical starting points for selecting AuNP diameter.
Particle Size by Application
Recommended Particle Size by Biomolecule Type
Antibody-gold and protein-gold conjugates for rapid diagnostic strip development and visible readout.
AuNP aggregation, plasmon shift, and signal amplification formats for molecular and immunoassays.
Oligonucleotide- and aptamer-functionalized AuNPs for hybridization, target recognition, and nucleic acid detection.
Functionalized AuNPs for electrochemical, optical, SPR, and affinity-based biosensor platforms.
Gold conjugates for microscopy, electron-dense labeling, cellular studies, and probe development.
Ligand-, peptide-, aptamer-, or antibody-modified AuNPs for targeted binding and delivery studies.
Surface plasmon peak position and broadening are monitored to evaluate conjugation and aggregation.
Hydrodynamic size and size distribution can be evaluated to confirm colloidal stability after conjugation.
Unbound biomolecule and excess reagents are removed using an appropriate purification method for the particle and biomolecule.
Conjugation efficiency, biomolecule loading, or depletion can be assessed depending on molecule type and assay needs.
Binding, hybridization, enzymatic activity, or assay-specific testing can be performed upon request.
Buffer compatibility, salt tolerance, aggregation behavior, and storage conditions can be evaluated for downstream use.
Gold nanoparticle conjugation is part of a broader nanomaterial and bioconjugation service platform.
This page covers custom gold nanoparticle conjugation, AuNP bioconjugation, oligonucleotide-gold conjugates, DNA-functionalized gold nanoparticles, antibody-gold nanoparticle conjugates, protein-AuNP conjugation, peptide-gold nanoparticle conjugation, PEGylated gold nanoparticles, lateral flow gold conjugates, biosensor nanoparticles, and diagnostic gold nanoparticle probes.
or the fastest review, send your biomolecule type, available functional groups, desired AuNP size, application, required quantity, buffer limitations, purity needs, and any assay performance requirements.
Selected references on gold nanoparticle bioconjugation, DNA-AuNP conjugates, antibody-gold probes, lateral flow assays, and nanoparticle characterization.
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