ISO 9001:2015
Bridged Nucleic Acids (BNA)
Bio-Synthesis offers accredited DNA Genotyping Services, following GLP guidelines for human cell line authentication and mycoplasma detection. These services are performed at accredited human DNA identity testing facilities by our team of scientists with years of experience in SNP, STR DNA profiling, high-resolution Luminex HLA DNA genotyping, DNA sequencing and bioinformatics data analysis. We provide our customers with the highest discriminating methods for species and identity confirmation, detection of cell cross-contamination and cell misidentification, assuring compliance with NIH recommendations and scholarly journal guidelines.
At our DNA Identity Testing Center, we make it easy and affordable to authenticate human cell lines and primary cells. Contact our customer service center or send an email to info@biosyn.com to find the right service package for your project.
$250/species
or
$1000 for all 14 species
The availability of well-characterized, viable human tissues and cell lines accompanied by pertinent donor data is a prerequisite for a great deal of current biomedical and transplantation research. Unfortunately, the misidentification and cross-contamination of human tissue cell lines occurs frequently. It is estimated that over 20% of all established cell lines are misclassified by cell type or species of origin, and over 33% of all cell lines suffer inter- or intra-species cross-contamination (1-3). The ATCC, along with the Coriell Institute for Medical Research, the European Collection of Cell Cultures (ECACC), the Deutsche Sammlung von Mikrorganismen and Zellkulturen (DSMZ), and the Japanese Collection of Research Resources have all received cell line submissions that, upon authentication, were determined to have been misidentified by the depositor (4,5). This poses a serious threat to the legitimacy of research findings produced from any of these cell cultures.
To aid clients in need of cell line identity validation, Bio-Synthesis (BSI) offers fast and reliable human cell line identification services to authenticate your cell lines. Scientists, with experience in STR DNA profiling, SNP genotyping, high-resolution Luminex HLA DNA typing, tissue microarray analysis and bioinformaticsdata analysis, perform DNA Identification at our accredited DNA testing facilities. Our commitment to total quality management assures our client's that our scientists employ efficient methods to confirming species' identity and detect cross-contamination, authenticating our client's cell lines in full compliance with NIH recommendations and scholarly journal guidelines.
If you have any questions or for additional information, please contact us or send an email to info@biosyn.com
Bio-Synthesis offers several different levels of cell authentication services at NO Additional Fees !
In addition, Bio-Synthesis offers microarray detection and analysis. This integrated service solution can be fully leveraged to generate top-quality results at the fastest possible turnaround time for your cancer cell and stem cell research needs.
Cell line contamination must be avoided to assure absolute reliability of the cell line authentication process. Cells used in basic and applied research can become altered in culture, either by contamination from other cell lines or by spontaneous genetic changes. The American Type Culture Collection (ATCC) recommends that researchers use low-passage cell lines and genotyping to authenticate cell line to assure reliable and reproducible results.
Cell line authentication and identification services from Bio-Synthesis are conducted under strict quality control processes.
Our GLP quality human cell line genotyping and characterization services offers rapid and accurate cell line identification by analyzing a pattern of defined short tandem repeats (STR). Cell line contamination is detected by the presence of 3 or more alleles using GeneMapper ID®Software.
Bio-Synthesis is committed to Total Quality Management (TQM) to assure our customers' complete satisfaction. QA (quality assurance) procedures are repeated for independent verification and are compliant with AABB and FDA regulations.
Dry Cell Pellets:
There is a minimum requirement of 100k cells if DNA has not been extracted. Please spin down the cells in an Eppendorf tube, wash the cell pellet with PBS buffer once and vac-dry without heating. Make sure the pellets are completely dry before shipping.
Frozen Cell Pellets:
There is a minimum requirement of 100k cells if DNA has not been extracted. Please spin down the cells in an Eppendorf tube, wash the cell pellet with PBS buffer once and immediately freeze it with liquid nitrogen. Keep frozen and ship in dry ice.
Cryopreservation Tube:
If your cell line tubes are preserved in liquid nitrogen, you may send one tube to us as-is in dry ice.
Extracted Genomic DNA:
Please provide at least 100 ng of DNA in 10 μL TE buffer or water. Include a 1% agarose gel picture to show DNA integrity.
Safety Guidelines:
Please make sure that the samples do not contain anything hazardous.
Sample Shipment:
When placing order online, please include cell line validation sample submission form with the sample.