Protein Biopolymer Conjugation Services
Bio-Synthesis, leader in providing custom protein expression services as well as
protein conjugation and modification with target molecule of your choice. We
are devoted to delivering affordable peptide-biopolymer hybrid complexes, and offer comprehensive modification and labeling options to be used for life science research,
diagnostics, and drug discovery.
Over the decades, we have accumulated a continually growing portfolio of peptide
modification and conjugation services. If you can't find what you want, contact
our National Customer Service Center at 800.220.0627, or contact
us online with your detailed project descriptions.
- Conjugation of protein to enzyme for immuno-assay
- Hapten conjugation to carrier protein for immunogen preparation
- Conjugation of poly(ethylene glycol) to protein
- Protein dextran modification
- Protein-drug conjugates for therapeutic drug delivery
- Protein-antibody conjugates for targeted delivery
- Protein-macrocyclic metal chelator as targeted medical imaging agents
- Protein-support immobilization for detection
- Bioconjugation of Protein Capture Agents to Biosensor Surfaces
- Protein glycopolymer conjugate
- Biotinylation of proteins for use with streptavidin- or avidin-based reagents
- Development of key properties of therapeutic proteins in the optimization of in-vitro and in-vivo performance
As each conjugation is unique, our support team will work closely with each client to identify their objective and determine proper coupling chemistry. As we are the
experts in protein bioconjugation techniques, Bio-SynthesisI can provide a properly conjugated product quickly and efficiently.
Protein Conjugation Service Descriptions
Coupling of preactivated small molecule and biomolecule
with chemical reactive groups such as amine, thiol, carboxylate, hydroxyl, aldehyde and ketone, active hydrogen, photo-chemical and cycloaddition reactions, zero-length
cross-linking, homobifunctional, heterobiofunctional, or multifunctional cross-linking chemistries, denrimer and dendrons, and cleavable reagent systems.
Some commonly used cross-linking reagents include:
- glutaraldehyde - links carrier molecules to N-terminus of peptide
- carbodiimide (EDC), - attaches carrier to C-terminus of peptide
- succinimide esters (e.g. MBS, SMCC) - binds free amino group and Cys residues
- benzidine (BDB), - links to Tyr residues
- periodate, - attaches to carbohydrate groups
After standard desalting, or purification,
a small percent of heterogeneous products containing single or multi-site conjugate
- Natural, recombinant protein, or synthetic peptides
- Protein: Enzyme, antibodies, antigens, cell adhesion molecules
- Peptides: Synthetic polypeptides
- Saccharides: Sugars, oligosaccharides and polysaccharides
- Lipids: Fatty acids, phospholipids, glycolipids and any fat-like substances.
- Ligands: Hormone receptors, cell surface receptors, avidin and biotin, small molecules
- Labels: Fluorescent dyes, infrared-absorbing and UV-Vis absorption chromophores,
- Nucleic acids and nucleotides: DNA, RNA, PNA, nucleic acid analogs and genomic DNA
- Synthetic polymers: PEG, Nanoparticles, gold particles, dendrimers, dendron, PAMAM
- Others: Conjugated or mixtures of any the above
- Soid Supports:agarose, glass plates, membrane, beads
All custom synthesis of biomolecules, modification,
or bioconjugation services are manufactured under strict quality control processes.Analytical HPLC and MS analyses are performed in every development cycle. Final
target conjugates must first be isolated from excess or unreacted reagent. In many cases, simple dialysis removes unreacted reagent from the reaction solution. Additional
purification such as stirred cell filtration, tangential flow filtration (TFF),
or gel filtration chromatography may also be used to either remove excess reagent
or to isolate and characterize the cross-linked product with an additional fee.
For reagents that are similar in size or larger than the antibody (mostly protein
and other biological molecules), other purification techniques such as affinity
chromatography, ion-exchange chromatography, and hydrophobic interaction chromatography.
The cross-linked target molecule may then be further characterized by biochemical
or biophysical techniques. Once the product has been purified, it may be subject
to various characterization methods including spectrometric (MALDI-TOF, ESI, LC-MS),fluorescence, electrophoresis, and immunochemical, biochemical, and enzymatic analysis.
QC (quality control) and QA (quality assurance) procedures are also followed independently to ensure the highest quality possible of every delivered conjugate. Moreover, our
dedicated technical account managers will guide your project through every step
of the process and keep you informed of the latest progress.
The typical delivery consists of lyophilized
sample in individual fully labeled vials. The shipment also contains COA, MS, HPLC and/or
other analytical data. Additional analytical data is also available upon request.