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Xtremer Single-Stranded DNA (ssDNA) Fragments

Xtremer Single-stranded DNA (ssDNA) fragments are important research tools in molecular biology. ssDNA can be used in diagnostic assays, CRISPR gene therapy, DNA nanotechnology and many other applications. ssDNA oligonucleotides are typically produced via well established chemical solid-phase DNA synthesis on polymeric supports. One advantage is that almost any type of nucleotide analog can be introduced at well defined positions. However, as the length is extended, the expected yields will diminish somewhat. To counter the diminished yield, Bio-Synthesis has developed enzymatic synthesis methods, allowing us to produce single-strand DNA reaching as much as 2000nt.

Bio-Synthesis has supplied synthetic single strand long DNA since 1984. The addition of Xtremer ssDNA fragments production by enzymatic methods is an expansion of our Molecular Biology services. Any length of ssDNA or dsDNA maybe requested, from modified monomers to long-range DNA in the multi-kilobase range.

Contact us or request a quote for Single-Stranded DNA Xtremer >400 bases.

Chemical DNA Synthesis vs DNA Synthesis by Enzymatic Method

DNA synthesis by enzymatic method is a powerful alternative to chemical DNA oligo synthesis and is useful when long DNA (>400 nt) is needed. Yields of chemically synthesized DNA decrease dramatically with increasing length. Thus with current chemistries, it is not efficient to synthesize long, high quality DNA chemically. We have therefore developed a unique enzymatic DNA synthesis method to produce long single stranded DNA (ssDNA) for lengths in the thousands of bases which are often used as repair templates in gene knockin experiments, in vitro transcription, and more.

  • Ready-to-use long ssDNA donor templates up to 2 kb in length
  • Verification by sequencing
  • Modifications available
  • TAT: 3-4 weeks