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Bio-Synthesis is a provider of Custom Oligo Synthesis services. Oligonucleotides are synthesized as unmodified or modified chemical structures with defined sequences using a procedure known as Phosphoramidite solid-phase synthesis. Oligos which are synthesized using solid-phase phosphoramidite chemistry are often purified by high performance liquid chromatography (HPLC) or PAGE to achieve the desired purity. Synthetic DNA or RNA oligonucleotides obtained by solid-phase synthesis are employed in a variety of molecular biology applications such as PCR, real-time PCR, sequencing, site directed mutagenesis, single nucleotide polymorphism (SNP) assays, microarrays and various therapeutic applications.
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DNA primers and probes
Long DNA oligos(up to 400 bases)
Other DNA bioconjugates
Short or long RNA oligos
Hybrid chimeric synthesis
Wide choice of modifications
Super functional synthetic RNA analogs
Replacement for LNA and PNA
Superior binding affinity
Long DNA Oligos (up to 400 bases)
Chemically synthesized DNA
Custom modifications available
Long RNA oligos (up to 300 bases)
Chimeric hybrid, modified oligos
Long RNA by chemical synthesis
Up to multi kilo-bases
5' capping, 3' Poly(A) Tailing
Multi gram size
Multi gram size
2'-OMe, 2'-F purified, Rnase free
Conjugating oligonucleotides with peptides, antibodies, proteins, dyes and small molecules.
Producing high quality oligonucleotides requires a rigorous quality control system in place. All the reagents used in our oligo synthesis process are sourced from reliable suppliers and checked for quality prior to use. During synthesis, we apply trityl monitoring of oligonucleotides to control the coupling efficiency at each cycle. All oligonucleotides are routinely analyzed by analytical HPLC or PAGE for purity and characterized by MALDI-TOF, LC-MS or ESI mass spectrometry. Finally, oligos are quantitated twice by UV spectrophotometry to provide an accurate measure of yield.
With nearly 30 years of experience in oligonucleotide synthesis, we have the expertise to synthesize the most technically challenging custom biomolecules. Our Molecular Biologists and Chemists are prepared to assist you with experimental design, application support, and troubleshooting.
As part of our long-standing commitment to quality, we employ the following standards for quality assurance:
QC documentation is available upon request including:
The following applies to mid-scale synthesis projects (200 mg - 10 g):