Bridged Nucleic Acids (BNA)
Biol-Synthesis offers multi-gram large scale oligonucleotide synthesis, competitive pricing and fast turnaround.
With over 30 years of experience in oligonucleotide services, Bio-Synthesis has been providing large-scale custom oligonucleotide synthesis for therapeutic projects worldwide. Using various synthesis technologies, we manufacture highly complex DNA and RNA oligonucleotides which are produced for high-throughput, in vivo or commercial applications in multi-gram quantities of highly purified antisense oligos, siRNAs, ribozymes, miRNA and qPCR primers and probes. All large scale oligonucleotides are manufacturing under strict quality management standards, which include MS, HPLC and gel electrophoresis after completion of synthesis, purification, and quality control steps. These steps are followed by independent QA (Quality Assurance) procedures which guarantee the highest quality large scale DNA or RNA oligonucletoides.
We are able to incorporate various types of modifications specifically for antisense or RNAi applications. The most popular modification used for these applications involves replacing Phophodiester with
Phosphothiolate linkages. Such linkages are highly resistant to nuclease degradation, but they also lower duplex stability. To compensate for such deficiency, we can incorporate modified bases which do not only minimize exonuclease degradation but also enhance duplex stability. See examples below.
Several types of purification are available
Since the salt content and the purity of oligonucleotides are important features during large scale synthesis, a desalting step is needed as an additional default purification method for this service.
Our In-Vivo-Ready purification option provides high quality oligonucleotides that are ready for animal studies. Each oligonucleotide is HPLC purified, dialyzed to ensure that the oligonucleotide is salt free (conductance of <1 microsiemens for a 50 um solution), sterile filtered, and endotoxin tested. All oligonucleotides are analyzed by MALDI-TOF mass spectrometry, analytical HPLC, and sequencing.
Several types of quality control
Our quality products are manufactured in compliance with GMP standards.
Our pricing applies to unmodified oligonucleotides between 15 and 25 bases. Oricing includes purifications and ethanol precipitation. Please request a quotation for other lengths or larger quantities. The backbone may consist of phosphorothioate or phosphodiester linkages, or a combination of the two. Prices may vary due to sequence composition.