Live Chat Support Software
800.227.0627
Circular Oligonucleotide
Circular Oligonucleotide Synthesis

Circular Oligonucleotide Synthesis

Single-stranded circular DNA or circular RNA oligonucleotides are designed to undergo triplex formation with single-stranded DNA and RNA targets. Many of these circular oligonucleotides bind with greater affinity, sequence selectivity and are more resistant to nucleases compared to their linear counterparts. These synthetic circular DNA or circular RNA oligonucleotides can be chemically synthesized at Bio-Synthesis using solution and solid-phase synthesis methods from partially protected linear precursors. After removing the linear DNA, circular oligonucleotides are purified by denaturing polyacrylamide gel electrophoresis (PAGE) with >95% purity. Circularity is determined by exonuclease cleavage. As a final step, we apply an independent QA procedures to guarantee the highest quality for each circular oligonucleotide.

Specs:

  • Length- 35 to 200 mers
  • Quantities- 100, 200, 500 pmole
  • Purification HPLC or PAGE purified
  • Validation- MS and PAGE analysis plus additional QA procedures

For additional information regarding custom circular DNA or RNA synthesis, contact us or send an email to info@biosyn.com

Circular Oligonucleotide - A New Concept for Molecular Diagnostic

Circular oligonucleotides act as an effective DNA or RNA template to produce long, repeating copies of circular sequences in rolling circle PCR application. Other strategies using circular oligonucleotide have shown interesting properties in diagnostic and therapeutic applications.

  • Anti sense using circular oligonucleotides
  • Binding of duplex DNA
  • Delivery vectors for miRNAs
  • Diagnostics
  • DNA fragment assembly using a nicking enzyme system
  • DNA polymerase inhibition
  • DNA structure studies
  • Efficient templates for DNA and RNA polymerases
  • Hairpin motif design
  • Hairpin studies
  • Ligation-independent cloning (LIC)
  • Manipulating gene expression with caged circular oligonucleotides
  • Mutation detection
  • Padlock probes
  • Probing DNA-protein interactions
  • Quantitation of sequence-dependent DNA bending and flexibility
  • RNA polymerase inhibition
  • Rolling Circle Amplification (RCA)
  • Single molecule counting
  • Specific gene expression
  • Study of noncanonical DNA structural motifs
  • Synthesis of concatemeric polypeptides
  • Topologic modifications
  • Triple helix formation
  • Unique DNA recognition properties

References

1. Diegelman, A. M. and Kool, E. T. (2000) Chemical and enzymatic methods for preparing circular single-stranded DNAs. In Current Protocols in Nucleic Acid Chemistry, vol. 2 (Beaucage, S. et al., eds.). John Wiley, New York, Chapter 5.2.

2. Oligonucleotide synthesis: methods and applications / edited by Piet Herdewijn. (Methods in molecular biology ; 288). ISBN 1-58829-233-

Custom Circular DNA, Circular RNA Synthesis

Bio-Synthesis custom DNA or custom RNA synthesis services offers numerous options to meet variety of research needs. We are committed to ISO quality management system to ensure our customers' complete satisfaction.

Case Study:

Sequence:91 base DNA Synthesis

TTC ACA GAG GAA GGG CCA GTA TCC TGT CCA AAC TTG ATG CTC CGG T CGT TTA GCA TAC TAA TCT GAG AGT CCG ACG GTC GTC AGT CAG TCA

Denaturing gel results:

Circular Oligonucleotide Synthesis

ESI Mass Spectra:

Linear 91 mer ssDNA, target MW: 28128.1, observed MW: 28126.9

Circular Oligonucleotide Synthesis

Circular 91 mer ssDNA, target MW: 28110.1, observed MW: 28111.1

Circular Oligonucleotide Synthesis

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please call 1-972-420-8505 or Fax at 1-972-420-0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."