Bridged Nucleic Acids (BNA)
Single-stranded circular DNA or circular RNA oligonucleotides are designed to undergo triplex formation with single-stranded DNA and RNA targets. Many of these circular oligonucleotides bind with greater affinity, sequence selectivity and are more resistant to nucleases compared to their linear counterparts. These synthetic circular DNA or circular RNA oligonucleotides can be chemically synthesized at Bio-Synthesis using solution and solid-phase synthesis methods from partially protected linear precursors. After removing the linear DNA, circular oligonucleotides are purified by denaturing polyacrylamide gel electrophoresis (PAGE) with >95% purity. Circularity is determined by exonuclease cleavage. As a final step, we apply an independent QA procedures to guarantee the highest quality for each circular oligonucleotide.
For additional information regarding custom circular DNA or RNA synthesis,contact us or send an email to firstname.lastname@example.org
Circular oligonucleotides act as an effective DNA or RNA template to produce long, repeating copies of circular sequences in rolling circle PCR application. Other strategies using circular oligonucleotide have shown interesting properties in diagnostic and therapeutic applications.
1. Diegelman, A. M. and Kool, E. T. (2000) Chemical and enzymatic methods for preparing circular single-stranded DNAs. In Current Protocols in Nucleic Acid Chemistry, vol. 2 (Beaucage, S. et al., eds.). John Wiley, New York, Chapter 5.2.
2. Oligonucleotide synthesis: methods and applications / edited by Piet Herdewijn. (Methods in molecular biology ; 288). ISBN 1-58829-233-
Bio-Synthesis custom DNA or custom RNA synthesis services offers numerous options to meet variety of research needs. We are committed to ISO quality management system to ensure our customers' complete satisfaction.
Sequence:91 base DNA Synthesis
TTC ACA GAG GAA GGG CCA GTA TCC TGT CCA AAC TTG ATG CTC CGG T CGT TTA GCA TAC TAA TCT GAG AGT CCG ACG GTC GTC AGT CAG TCA
Linear 91 mer ssDNA, target MW: 28128.1, observed MW: 28126.9
Circular 91 mer ssDNA, target MW: 28110.1, observed MW: 28111.1