Circular Oligonucleotide - A New Concept for Molecular Diagnostic
Circular oligonucleotides act as an effective DNA or RNA template to produce long, repeating copies of circular sequences in rolling circle PCR application. Other strategies using circular oligonucleotide have shown interesting properties in diagnostic and therapeutic applications.
- Anti sense using circular oligonucleotides
- Binding of duplex DNA
- Delivery vectors for miRNAs
- Diagnostics
- DNA fragment assembly using a nicking enzyme system
- DNA polymerase inhibition
- DNA structure studies
- Efficient templates for DNA and RNA polymerases
- Hairpin motif design
- Hairpin studies
- Ligation-independent cloning (LIC)
- Manipulating gene expression with caged circular oligonucleotides
- Mutation detection
- Padlock probes
- Probing DNA-protein interactions
- Quantitation of sequence-dependent DNA bending and flexibility
- RNA polymerase inhibition
- Rolling Circle Amplification (RCA)
- Single molecule counting
- Specific gene expression
- Study of noncanonical DNA structural motifs
- Synthesis of concatemeric polypeptides
- Topologic modifications
- Triple helix formation
- Unique DNA recognition properties
References
1. Diegelman, A. M. and Kool, E. T. (2000) Chemical and enzymatic methods for preparing circular single-stranded DNAs. In Current Protocols in Nucleic Acid Chemistry, vol. 2 (Beaucage, S. et al., eds.). John Wiley, New York, Chapter 5.2.
2. Oligonucleotide synthesis: methods and applications / edited by Piet Herdewijn. (Methods in molecular biology ; 288). ISBN 1-58829-233-
Custom Circular DNA, Circular RNA Synthesis
Bio-Synthesis custom DNA or custom RNA synthesis services offers numerous options to meet variety of research needs. We are committed to ISO quality management system to ensure our customers' complete satisfaction.
Case Study:
Sequence:91 base DNA Synthesis
TTC ACA GAG GAA GGG CCA GTA TCC TGT CCA AAC TTG ATG CTC CGG T CGT TTA GCA TAC TAA TCT GAG AGT CCG ACG GTC GTC AGT CAG TCA
Denaturing gel results:
ESI Mass Spectra:
Linear 91 mer ssDNA, target MW: 28128.1, observed MW: 28126.9
Circular 91 mer ssDNA, target MW: 28110.1, observed MW: 28111.1
