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Bio-Synthesis offers positive RNA control for rapid detection of SARS-CoV-2 (2019-nCoV) virus. Our control has been used by FDA approved labs to carry out COVID-19 molecular testing. It covers 3 separated regions on the SARS-CoV-2 viral genome and can be detected by RT-qPCR assays of SARS-CoV-2 N1 and SARS-CoV-2 N2. All oligonucleotide primers and probes are highly purified.

COVID19

Ready-to-Use Cocktail Mix of 2019-nCOV CDC qPCR Assay

Reagent Part # Description Reactions /Tube User Instruction
Positive Template Control (SARS2PC) SARS2PC-1000 Positive Template Control (SARS2PC) 1,000 1.Dissolve in 100 uL RNA storage solution. freeze to -70°C or below.
2. For use, this stock is diluted 1:100 (to 1,000 gene copies/uL) with extracted nucleic acid from human embryonic lung cells which contain RNase P RNA.
3. For 20 uL RT-qPCR, use 5 uL diluted SARS2PC.
SARS-CoV-2 N1 Oligo Mix SARS-CoV2-N1-FRP  1) SARS-CoV-2 N1 forward primer;
2) SARS-CoV-2 N1 Reverse Primer;
3) SARS-CoV-2 N1 Probe
1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved Oligo Mix.
SARS-CoV-2 N2  Oligo Mix SARS-CoV2-N2FRP  1) SARS-CoV-2 N2 forward primer;
2) SARS-CoV-2 N2 Reverse Primer;
3) SARS-CoV-2 N2 Probe
1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved Oligo Mix.
Human RNase P (RP) Oligo Mix Human-Rnase-FRP 1)Human RNase P (RP) Forward Primer;
2) Human RNase P (RP) Reverse Primer;
3) Human RNase P (RP) Probe
1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved Oligo Mix.

List of available probe and primer:

Reagent Part # Description Reactions /Tube User Instruction
Positive Template Control (SARS2PC) SARS2PC-1000 Positive Template Control (SARS2PC) 1,000 1.Dissolve in 100 uL RNA storage solution. freeze to -70°C or below.
2.For use, this stock is diluted 1:100 (to 1,000 gene copies/uL) with extracted nucleic acid from human embryonic lung cells which contain RNase P RNA.
3. For 20 uL RT-qPCR, use 5 uL diluted SARS2PC.
SARS-CoV-2 N1 Forward Primer SARS-CoV2-N1-F SARS-CoV-2 N1 forward primer  1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved oligo.
SARS-CoV-2 N1 Reverse Primer SARS-CoV2-N1-R SARS-CoV-2 N1 Reverse Primer 1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved oligo.
SARS-CoV-2 N1 Probe SARS-CoV2-N1-P SARS-CoV-2 N1 Probe 1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.08 uL dissolved oligo.
SARS-CoV-2 N2 Forward Primer SARS-CoV2-N2-F SARS-CoV-2 N2 forward primer  1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved oligo.
SARS-CoV-2 N2 Reverse Primer SARS-CoV2-N2-R SARS-CoV-2 N2 Reverse Primer 1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved oligo.
SARS-CoV-2 N2 Probe SARS-CoV2-N2-P SARS-CoV-2 N2 Probe 1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.08 uL dissolved oligo.
Human RNase P (RP) Forward Primer Human-RNase-F Human RNase P (RP) Forward Primer 1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved oligo.
Human RNase P (RP) Reverse Primer Human-RNase-R Human RNase P (RP) Reverse Primer 1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.72 uL dissolved oligo.
Human RNase P (RP) Probe Human-RNase-F\P Human RNase P (RP) Probe 1,000 1.Dissolve in 400 ul PCR water.
2.For 20 uL RT-qPCR, use 0.08 uL dissolved oligo.

Additional Informations: