Bridged Nucleic Acids (BNA)
The ability to synthesize target RNA, such as mRNA, in the laboratory is critical
to certain molecular biology techniques and genomic studies including ribozyme and
aptamer synthesis, RNA structure and processing analyses, RNA interference and antisense
RNA techniques. Bio‑Synthesis is the only company that offers the full range
of services to manufacture high quality ultra
long RNA synthetically or by in vitro transcription from template options
including Plasmids, PCR Products, oligonucleotides and cDNA. Whether you need research-grade
RNA, a control template for molecular assays or a modified capped RNA, Bio‑Synthesis
has the track record and expertise in providing high quality RNA, along with flexibility
which is unmatched by other suppliers.
All d2RNATM, in vivo transcription RNA, are delivered fully purified
using various purification options and quality checked including, but not limit
to, DNA sequencing, DNA, RNA size checked by electrophoresis, or real-time PCR,
qPCR analysis. Custom assay development and validation are also available upon request.
Final RNA oligos are delivered normalized and packaged lyophilized in an inert chemical
matrix that inactivates trace RNase in the liquid phase, providing an added level
of RNA stability during sample handling, with or without ice.
While there has been significant demand for ultra long RNA oligonucleotides for
gene structure and functional studies, the synthesis of long RNA fragments using
the conventional RNA synthesis method is highly sequence-dependent. This is due
to the potential for secondary structure formation and the inherent difficulty for
chemical synthesis with additional 2'-OH reactive group of RNA introduces considerable
complexity to the synthesis. Usually the conventional method can only synthesize
RNA fragments under 100 bases. Bio‑Synthesis has met the challenge of synthesizing
thousands of semi-synthetic long RNA, d2RNATM (3000 bases and more) using
the best reagents available, proprietary methods to optimize the RNA manufacturing
process, and the expertise in nucleic acid chemistry and molecular biology since
Each in vitro transcribed long RNA oligo is meticulously monitored during synthesis
and controlled according to Bio-Synthesis’ stringent quality assurance and
quality control standards. Each DNA template can be verified with bidirectional
DNA sequencing in multiple locations. BSI’s Quality Control department will ensure
the final product is verified and passes all standards.
For over 25 years, Bio‑Synthesis has maintained these gold standards for long
RNA oligonucleotide synthesis that saves the researcher a great deal of time and
trouble for applications that require direct synthesis of the entire target fragment
such as mRNA.
These long oligos can be used in variety of molecular biology applications such
Contact us today to discuss your
Long RNA project.
All Bio‑Synthesis RNA transcript oligos are purified under RNase free environment.
Purification methods include:
Yield will vary based upon base composition, purification, oligo length, scale and
modifications. That's why we have a set guaranteed quantity.
Each step of the RNA production process is carefully monitored, starting from oligonucleotide
synthesis to DNA templates production. Each step is screened by mass spectrometry,
agarose size check and bidirectional DNA sequencing in multiple locations. After
transcription, the RNA is carefully checked using denaturing PAGE electrophoresis,
Real-Time PCR and other additional custom assay developments as required by our
If you are dissatisfied and report an oligo problem within 30 days of order receipt,
we will gladly remake your oligo free of charge or provide a credit for the purchase
All orders are shipped with a certificate of analysis and other fully traceable
Bio‑Synthesis provides custom d2RNATM, in vitro transcribed long
RNA (>3000 and more) and messenger RNA (mRNA) synthesis service and modifications
with flexible synthesis scales for your individual research needs. The RNA probes
produced at Bio‑Synthesis have been used as radio-labeled and nonisotopically
labeled RNA probes in blot hybridization and nuclease protection assays. Modified
RNA probes are used in various biochemical and molecular biology studies. d2RNA
are also used as control template for gene expression analysis (microinjection,
infection with viral transcripts, in vitro translation), structure analysis (protein-RNA
binding), and RNA-based diagnostic testing and drug development.
Contact us now to discuss your
specific RNA synthesis projects.