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Bio-Synthesis offers a complete array of fluorescent reporters and quenchers. Our product offering includes common dye modifiers such as 6-FAM, HEX and TET, as well as exotic modifications. We can directly attach fluorescent dyes to oligos at the 5'-end, 3'-end and even internal positions of an oligonucleotides during synthesis. When certain modifications (such as Digoxigenin and some fluorescent dyes) are not available for direct incorporation, cross-linking two molecules by using a specific functional group such as an amine, thiol modified oligonucleotide with certain cross linkers, are employed. See Chemistry behind cross linking Post-synthetic chemical modifications made to an oligonucleotide result in lower yields than modifications introduced during synthesis. Furthermore, all post synthetic conjugations require HPLC purification. PAGE purification is not offered for post synthetic conjugation as yields are further decreased and certain modifications can be damaged during PAGE purification.

All qPCR probes are available at a fixed price per oligonucleotide, within the following parameters:

  • Synthesis of the custom oligos up to 30 bases in length
  • Deprotected, desalted and HPLC purified (>85% pure)
  • Wide range of dye and quencher options available
  • QC by MALDI-TOP Mass Spectrometry, PAGE and Fluoroscan
  • 3-10 working days turnaround, pending successful QC validation
  • Assistance with the design is available, free of charge

Popular Probes

5'-Label 3'-Label Scale Minimum Yields Price
5' CAL Fluor Gold 540 3' BHQ™ 1 5 nmol 5 nmol pure $195
5' CAL Fluor Gold 540 3' BHQ™ 1 250 nmol 25 nmol pure $275
5' CAL Fluor Gold 540 3' BHQ™ 1 1 µmol 50 nmol pure $400
5' CAL Fluor Orange 560 3' BHQ™ 1 100 nmol 5 nmol pure $195
5' CAL Fluor Orange 560 3' BHQ™ 1 250 nmol 25 nmol pure $275
5' CAL Fluor Orange 560 3' BHQ™ 1 1 µmol 50 nmol pure $400
5' DYOMICS Dyes NHS 3' BHQ™ 1 250 nmol 8 nmol pure $550
5' DYOMICS Dyes NHS 3' BHQ™ 1 1 µmol 20 nmol pure $660
5' FAM 3' BHQ™ 1 100 nmol 5 nmol pure $195
5' FAM 3' BHQ™ 1 250 nmol 25 nmol pure $265
5' FAM 3' BHQ™ 1 1 µmol 50 nmol pure $395
5' HEX 3' BHQ™ 1 100 nmol 5 nmol pure $245
5' HEX 3' BHQ™ 1 250 nmol 25 nmol pure $325
5' HEX 3' BHQ™ 1 1 µmol 50 nmol pure $490
5' JOE 3' BHQ™ 1 250 nmol 8 nmol pure $325
5' JOE 3' BHQ™ 1 1 µmol 20 nmol pure $490
5' Rhodamine-6G NHS 3' BHQ™ 1 250 nmol 8 nmol pure $450
5' Rhodamine-6G NHS 3' BHQ™ 1 1 µmol 20 nmol pure $630
5' TET 3' BHQ™ 1 100 nmol 5 nmol pure $235
5' TET 3' BHQ™ 1 250 nmol 25 nmol pure $325
5' TET 3' BHQ™ 1 1 µmol 50 nmol pure $490
5' Yakima Yellow 3' BHQ™ 1 100 nmol 5 nmol pure $260
5' Yakima Yellow 3' BHQ™ 1 250 nmol 10 nmol pure $365
5' Yakima Yellow 3' BHQ™ 1 1 µmol 20 nmol pure $545
5' CAL Fluor Red 590 3' BHQ™ 2 100nmol 5 nmol pure $195
5' CAL Fluor Red 590 3' BHQ™ 2 250 nmol 25 nmol pure $270
5' CAL Fluor Red 590 3' BHQ™ 2 1 µmol 50 nmol pure $390
5' CAL Fluor Red 610 3' BHQ™ 2 100 nmol 5 nmol pure $215
5' CAL Fluor Red 610 3' BHQ™ 2 250 nmol 25 nmol pure $315
5' CAL Fluor Red 610 3' BHQ™ 2 1 µmol 50 nmol pure $475
5' CAL Fluor Red 635 3' BHQ™ 2 100 nmol 2 nmol pure $255
5' CAL Fluor Red 635 3' BHQ™ 2 250 nmol 10 nmol pure $375
5' CAL Fluor Red 635 3' BHQ™ 2 1 µmol 40 nmol pure $550
5' Cy3® 3' BHQ™ 2 1 µmol 20 nmol pure $530
5' Cy3® 3' BHQ™ 2 100 nmol 2 nmol pure $250
5' Cy3® 3' BHQ™ 2 250 nmol 8 nmol pure $355
5' Cy5® 3' BHQ™ 2 100 nmol 2 nmol pure $260
5' Cy5® 3' BHQ™ 2 250 nmol 8 nmol pure $365
5' Cy5® 3' BHQ™ 2 1 µmol 20 nmol pure $545
5' Dragonfly Orange 3' BHQ™ 2 100 nmol 2 nmol pure $325
5' Dragonfly Orange 3' BHQ™ 2 250 nmol 8 nmol pure $400
5' Dragonfly Orange 3' BHQ™ 2 1 µmol 15 nmol pure $570
5' FAM 3' BHQ™ 2 100 nmol 5 nmol pure $195
5' FAM 3' BHQ™ 2 250 nmol 25 nmol pure $265
5' FAM 3' BHQ™ 2 1 µmol 50 nmol pure $395
5' HEX 3' BHQ™ 2 100 nmol 5 nmol pure $245
5' HEX 3' BHQ™ 2 250 nmol 25 nmol pure $325
5' HEX 3' BHQ™ 2 1 µmol 50 nmol pure $490
5' JOE 3' BHQ™ 2 250 nmol 8 nmol pure $325
5' Quasar 570 3' BHQ™ 2 100 nmol 5 nmol pure $215
5' Quasar 570 3' BHQ™ 2 250 nmol 25 nmol pure $315
5' Quasar 570 3' BHQ™ 2 1 µmol 50 nmol pure $450
5' Quasar 670 3' BHQ™ 2 100 nmol 5 nmol pure $230
5' Quasar 670 3' BHQ™ 2 250 nmol 20 nmol pure $315
5' Quasar 670 3' BHQ™ 2 1 µmol 40 nmol pure $475
5' Quasar 705 3' BHQ™ 2 100 nmol 2 nmol pure $230
5' Quasar 705 3' BHQ™ 2 250 nmol 10 nmol pure $315
5' Quasar 705 3' BHQ™ 2 1 µmol 40 nmol pure $475
5' ROX 3' BHQ™ 2 250 nmol 10 nmol pure $450
5' ROX 3' BHQ™ 2 1 µmol 20 nmol pure $690
5' TAMRA NHS Ester 3' BHQ™ 2 100nmol 2 nmol pure $225
5' TAMRA NHS Ester 3' BHQ™ 2 250 nmol 8 nmol pure $425
5' TAMRA NHS Ester 3' BHQ™ 2 1 µmol 15 nmol pure $595
5' TET 3' BHQ™ 2 100 nmol 5 nmol pure $275
5' TET 3' BHQ™ 2 250 nmol 15 nmol pure $325
5' TET 3' BHQ™ 2 1 µmol 25 nmol pure $490
5' Texas Red NHS 3' BHQ™ 2 250 nmol 5 nmol pure $430
5' Texas Red NHS 3' BHQ™ 2 1 µmol 15 nmol pure $660
5' Cy5® 3' BHQ™ 3 100 nmol 2 nmol pure $275
5' Cy5® 3' BHQ™ 3 250 nmol 5 nmol pure $365
5' Cy5® 3' BHQ™ 3 1 µmol 15 nmol pure $545
5' ATTO-TEC NHS 3' BHQ™1 250 nmol 8 nmol pure $500
5' ATTO-TEC NHS 3' BHQ™1 1 µmol 20 nmol pure $660
5' ATTO-TEC NHS 3' Eclipse® DQ 250 nmol 8 nmol pure $500
5' ATTO-TEC NHS 3' Eclipse® DQ 1 µmol 20 nmol pure $660
5' Cy3® or Cy5® 3' Eclipse® DQ 100 nmol 2 nmol pure $280
5' Cy3® or Cy5® 3' Eclipse® DQ 250 nmol 8 nmol pure $350
5' Cy3® or Cy5® 3' Eclipse® DQ 1 µmol 20 nmol pure $520
5' Dragonfly Orange 3' Eclipse® DQ 100 nmol 2 nmol pure $325
5' Dragonfly Orange 3' Eclipse® DQ 250 nmol 8 nmol pure $400
5' Dragonfly Orange 3' Eclipse® DQ 1 µmol 20 nmol pure $570
5' DYOMICS Dyes 3' Eclipse® DQ 250 nmol 5 nmol pure Inquire
5' DYOMICS Dyes 3' Eclipse® DQ 1 µmol 15 nmol pure Inquire
5' FAM 3' Eclipse® DQ 250 nmol 5 nmol pure $290
5' FAM 3' Eclipse® DQ 1 µmol 15 nmol pure $490
5' HEX 3' Eclipse® DQ 250 nmol 5 nmol pure $325
5' HEX 3' Eclipse® DQ 1 µmol 15 nmol pure $490
5' JOE 3' Eclipse® DQ 250 nmol 5 nmol pure $325
5' JOE 3' Eclipse® DQ 1 µmol 15 nmol pure $490
5' Rhodamine-6G NHS Ester 3' Eclipse® DQ 1 µmol 8 nmol pure $355
5' Rhodamine-6G NHS Ester 3' Eclipse® DQ 1 µmol 15 nmol pure $535
5' TAMRA NHS Ester 3' Eclipse® DQ 250 nmol 8 nmol pure $355
5' TAMRA NHS Ester 3' Eclipse® DQ 1 µmol 15 nmol pure $535
5' TET 3' Eclipse® DQ 250 nmol 8 nmol pure $295
5' TET 3' Eclipse® DQ 1 µmol 20 nmol pure $495
5' Yakima Yellow 3' Eclipse® DQ 250 nmol 8 nmol pure $400
5' Yakima Yellow 3' Eclipse® DQ 1 µmol 20 nmol pure $573
5' FAM 3' dT-BHQ-1/3’ C3 250 nmol 10 nmol pure $400
5' FAM 3' dT-BHQ-1/3’ C3 1 µmol 25 nmol pure $515
5' BHQ-2 3' Pulsar 650 250 nmol 10 nmol pure $450
5' BHQ-2 3' Pulsar 650 1 µmol 20 nmol pure $660
5' FAM 3' TAMRA NHS 100 nmol 2 nmol pure $225
5' FAM 3' TAMRA NHS 250 nmol 8 nmol pure $425
5' FAM 3' TAMRA NHS 1 µmol 15 nmol pure $595
5' HEX 3' TAMRA NHS 100 nmol 2 nmol pure $225
5' HEX 3' TAMRA NHS 250 nmol 8 nmol pure $425
5' HEX 3' TAMRA NHS 1 µmol 15 nmol pure $595
5' TET 3' TAMRA NHS 100 nmol 2 nmol pure $225
5' TET 3' TAMRA NHS 250 nmol 8 nmol pure $425
5' TET 3' TAMRA NHS 1 µmol 15 nmol pure $595
5' Yakima Yellow 3' TAMRA NHS 250 nmol 8 nmol pure $425
5' Yakima Yellow 3' TAMRA NHS 1 µmol 15 nmol pure $595