Long Oligo Synthesis - DNA UbermerTM
While there has been significant demand for long oligos (150 mer and more), the
yields in conventional DNA synthesis and the associated side reactions have previously
limited the availability of oligonucleotides to lengths <150 nt. Bio-Synthesis's expertise in artificial gene synthesis provides us with the ability to synthesize thousands of single strand synthetic long DNA oligos using the best DNA synthesis reagents available and optimized long oligo synthesis protocols which permits high quality DNA Ubermer with superior coupling
efficiency of > 99.5% through an entire DNA synthesis reaction. Each custom
DNA long oligo is meticulously monitored during synthesis and controlled according to
Bio-Synthesis’s stringent quality assurance and quality control standards. The final
product is identified by LC-MS Electrospray, sequencing or other custom analytical
methods. Purity is analyzed by high throughput capillary gel or PAGE electrophoresis.
For over 29 years Bio-Synthesis has maintained these gold standards for long DNA
oligonucleotide synthesis that saves the researcher a great deal of time and trouble
for applications that require direct synthesis of the entire target fragment.
Application of DNA Long Oligonucleotide
These long oligos can be used in variety of molecular biology application including but not limited to:
- Cloning, gene construction and site directed mutagenesis
- Preparation of shRNA libraries
- Templates for RNA synthesis using in-vitro transcription
- SNP synthetic positive control template for qPCR
- Targeted resequencing
- Other high complexity synthetic biology applications
All Bio-Synthesis's oligos include desalting and deprotection at no additional charge.
We strongly recommend oligonucleotide longer than 50 bases to be purified by poly
acrylamide gel electrophoresis (PAGE). If you are unsure about the purification
level you need for your research, see our technical bulletin titled
What type of purification I need for my applications or
contact us for assistance.
Unlike other suppliers, there is no additional charge for DNA Oligo Ubermer Synthesis.
Yield will vary based upon base composition, purification, oligo length, scale and
modifications. That's why we have a set guaranteed quantity.
All custom oligo synthesis are routinely checked for length, purity, and composition. Long oligos are
typically quality checked by PAGE, ESI-MS, and/or CE. Our global quality control
capabilities include MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry
(ESI-MS), capillary electrophoresis (CE), and polyacrylamide gel electrophoresis
(PAGE). If you are dissatisfied and report an oligo problem within 30 days of order
receipt, we will gladly remake your oligo free of charge or provide a credit for
the purchase amount.
All orders are shipped with a Quality Assurance document that includes measured
yields, melting temperature, MW and µg/OD with every oligo.
Ultra Long Oligo Synthesis
Bio-Synthesis provides synthesis of oligo extendamer and modification with flexible
synthesis scales for your individual research needs. These long oligo are suitable
applications such as cloning or side-directed mutagenesis. Longer constructs that
required more choices like palindromes for restriction enzymes give researchers
more flexibility. DNA Ubermers are also used as an internal control template with
or without certain SNP's and has been use in amplification reactions like Real-Time
PCR and qPCR.
Specifications of DNA UbermerTM
- Synthesis of up to 400 bases
- All desalted or/and purified by gel purified
- QC by Sequencing
- Delivered normalized and lyophilized in tubes or plates
- Ideal for gene construction, targeted resequencing, control templates and other
complex synthetic biology
* Yields: Sequence complexity results in reduce yield