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Bio-Synthesis offers high throughput long oligo synthesis services often used in control
templates, cloning, mutagenesis, gene expression and other demanding applications.
Our track record in providing thousands of high-fidelity long oligo synthesis
up to 400 bases is unmatched.
Long DNA Oligos are delivered deprotected, desalted to remove small
molecular impurities, and PAGE purified for extremely high purity. Long DNA Oligos are delivered lyophilized and
normalized guarantying yields of 5 or 10 nmole.
Need kilobase ssDNA for gene knockin? Visit our Xtremer Single Strand DNA Synthesis page
While there has been significant demand for long oligos (150 mer and more), the
yields in conventional DNA synthesis and the associated side reactions have previously
limited the availability of oligonucleotides to lengths <150 nt. Bio-Synthesis's
expertise in artificial gene synthesis provides us with
the ability to synthesize thousands of single strand synthetic long DNA oligos using the best DNA synthesis reagents available and optimized long oligo
synthesis protocols which permits high quality DNA Ubermer with superior coupling
efficiency of > 99.5% through an entire DNA synthesis reaction. Each custom
DNA long oligo is meticulously monitored during synthesis and controlled according to
Bio-Synthesis’s stringent quality assurance and quality control standards. The final
product is identified by LC-MS Electrospray, sequencing or other custom analytical
methods. Purity is analyzed by high throughput capillary gel or PAGE electrophoresis.
For over 29 years Bio-Synthesis has maintained these gold standards for long DNA
oligonucleotide synthesis that saves the researcher a great deal of time and trouble
for applications that require direct synthesis of the entire target fragment.
These long oligos can be used in variety of molecular biology application including
but not limited to:
All Bio-Synthesis's oligos include desalting and deprotection at no additional charge.
We strongly recommend oligonucleotide longer than 50 bases to be purified by poly
acrylamide gel electrophoresis (PAGE). If you are unsure about the purification
level you need for your research, see our technical bulletin titled
What type of purification I need for my applications or
contact us for assistance.
Unlike other suppliers, there is no additional charge for DNA Oligo Ubermer Synthesis.
Yield will vary based upon base composition, purification, oligo length, scale and
modifications. That's why we have a set guaranteed quantity.
All custom oligo synthesis are routinely checked for length, purity, and composition. Long oligos are
typically quality checked by PAGE, ESI-MS, and/or CE. Our global quality control
capabilities include MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry
(ESI-MS), capillary electrophoresis (CE), and polyacrylamide gel electrophoresis
(PAGE). If you are dissatisfied and report an oligo problem within 30 days of order
receipt, we will gladly remake your oligo free of charge or provide a credit for
the purchase amount.
All orders are shipped with a Quality Assurance document that includes measured
yields, melting temperature, MW and µg/OD with every oligo.
Bio-Synthesis provides synthesis of oligo extendamer and modification with flexible
synthesis scales for your individual research needs. These long oligo are suitable
applications such as cloning or side-directed mutagenesis. Longer constructs that
required more choices like palindromes for restriction enzymes give researchers
more flexibility. DNA Ubermers are also used as an internal control template with
or without certain SNP's and has been use in amplification reactions like Real-Time
PCR and qPCR.
* Yields: Sequence complexity results in reduce yield