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Custom DNA Synthesis
Custom DNA Synthesis

Bio-Synthesis is a provider of Custom DNA Synthesis services tailored to our customers specifications. Our DNA Oligo synthesis scale ranges simple to complex, multi-gram scale up to 400 bases. We offer an extensive range of DNA oligonucleotide modifications including DNA backbone modifications, modified bases and fluorescent dye labeling. These modified oligonucleotides conform to the highest quality specifications for research, diagnostics, OEM and therapeutics.

DNA oligos are deprotected and desalted using SEC chromatography to remove impurities causes by trace molecules as part of our HPLC or PAGE purification process. Oligos are quantified twice by UV spectrophotometry to provide an accurate measure of OD yield. As part of our quality control process, DNA oligos are characterized by MALDI, or ESI Mass spectrophotometry and their purity is checked by analytical HPLC/PAGE.

DNA Synthesis Services:

  • Synthetic DNA is deprotected, desalted, lyophilized, Ready-to-Use
  • Quantitated 2x by UV spectrophotometry to provide an accurate measure of yield
  • Synthetic DNA is checked for quality by MALDI-TOF mass spectrometry
  • Synthetic DNA is checked for purity by HPLC and/or PAGE
  • Quality Assurance document includes: yields, TM, MW, μg/OD and more...
  • Fast delivery and high throughput capacity

Optional DNA Services:

DNA Services Description:

Unmodified DNA Synthesis

We offer several scales of custom unmodified DNA synthesis at very competitive prices. From small scale (custom DNA for research) to large production of ASR and GMP DNA oligomers. More Information...

Long Oligonucleotide Synthesis

Long DNA, as an internal control template with or without certain SNP's is used in amplification reactions such as Real-Time PCR and qPCR. More Information...

Custom Arrays/Libraries

High-throughput synthetic DNA can be provided in 96 or 384 well plates. We can customize the plate type required for your system. Options for oligos in solution include equimolar concentration, forward and reverse primer in the same well, dilution in buffer or water, and equal or variable volume. Options for lyophilized oligos in plates include equal nmole amounts or µg amount. More Information...

DNA Modifications

We provide thousand of custom DNA modifications such as DNA methylation, DNA base modification, backbone modification, and many others. More Information...

DNA qPCR Probes

We offer a wide variety of fluorescent reporters and quenchers including common dye modifiers such as 6-FAM, HEX and TET, as well as a full range of exotic modifications. More Information...

Antisense Oligos

Antisense oligos are powerful tools to halt biological events such as transcription, translation or splicing. We offer hybrid synthesis of RNA-DNA duplexes with mixed backbone linkages. More Information...

Oligo Chimera

Synthesis of chimeric DNA, chimeric DNA/RNA, chimeric DNA-peptide conjugates provides companies and researchers with powerful tools for research and pharmaceutical applications. More Information...

Custom DNA Synthesis

2' Deoxy DNA Oligonucleotide Synthesis
Linkages Scale Estimate Yields Price Restrictions
Phosphodiester (PO)
$10 Minimum (25 to 50 nmole)
$20 Minimum (100 to 250 nmole)
$60 Minimum (1 to 15 µmole)
50 nmole 3-5 ODs $0.42/base 15-60 bases
100 nmole 5-10 ODs $0.85/base 10-90 bases
250 nmole 15-20 ODs $1.00/base 5-100 bases
1 µmole 20-60 ODs $1.85 /base 5-100 bases
2 µmole 40-120 ODs $2.95 /base 5-100 bases
5 µmole 100-300 ODs $9.00/base 5-100 bases
10 µmole 200-600 ODs $12.00/base 5-100 bases
15 µmole 300-750 ODs $15.00/base 5-100 bases
Phosphorothioate (PS)
$40 Minimum
250 nmole 15 ODs $3.50/bond 10-25 base
1 µmole 20-60 ODs $5.50/bond 10-25 base
5 µmole 100-300 ODs $20.00/bond 10-25 base
10 µmole 200-600 ODs $35.00/bond 10-25 base
15 µmole 300-750 ODs Inquire 10-25 base
Methylphosphonate (MPN)
$150 minimum
1 µmole 20-60 ODs $35.00/bond 10-25 base
5 µmole 100-300 ODs $85.00/bond 10-25 base
10 µmole 200-600 ODs $125.00/bond 10-25 base
15 µmole 300-750 ODs Inquire 10-25 bases
  • PO - Phosphodiester, PS - phosphorothioates, MPN - Methylphosphonateli
  • Estimated yield is for 20 to 50 bases. Purity and yield may vary based on oligo length, modifications, or sequence composition.

Click here for recommended purification and applications

Click here for information regarding methods of purification

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Purifications of DNA Oligonucleotides
Purifications 100 nmol 250 nmol 1 umol 5 umol 10 umol
PAGE $65 $95 $125 $675 $965
IE HPLC $55 $85 $115 $240 $395
RP HPLC $55 $85 $115 $240 $395
Dual HPLC $100 $135 $195 $500 $700
Dual PAGE/HPLC $105 $160 $200 Inquire Inquire
RNase-Free PAGE $105 $135 $190 $395 $625
RNase-Free HPLC $85 $115 $160 $340 $590
RNase-Free Dual HPLC $135 $165 $225 $540 $785
RNase- Free Dual PAGE & HPLC NA $195 $295 Inquire inquire
  • The above estimated yields are for 20 to 50 bases in length. Purity and yield may vary based on oligo length, modifications, or sequence composition.
  • An additional 1-2 days of turnaround time is required for purification.

Click here for information regarding methods of purification

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Estimated Yields of DNA Oligonucleotides
Purifications 100 nmol 250 nmol 1 umol 5 umol 10 umol
PAGE 0.5-1 OD 2 ODs 10 ODs 50 ODs 100 ODs
IE HPLC 1-2 OD 4 ODs 20 ODs 100 ODs 200 ODs
HPLC 1-2 OD 4 ODs 20 ODs 100 ODs 200 ODs
RNase-Free HPLC 1-2 OD 4 ODs 20 ODs 100 ODs 200 ODs
Dual HPLC 0.5-1 OD 2 ODs 10 ODs 50 ODs 100 ODs
Dual PAGE & HPLC NA 1 ODs 5 ODs 25 ODs 50 ODs
  • Above estimated yield is for 20 to 50 bases in length. Purity and yield guarantees may vary due to oligo length, modifications, or sequence composition.
  • An additional 1-2 days of turnaround time is required for purification.

Click here for information regarding methods of purification

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Custom DNA Synthesis Case Study

At Bio-Synthesis we have developed methods for synthesizing complex and long oligos. Below, you will find a custom oligo synthesis case study representing our capability to synthesize long DNA & RNA, both chemically or enzymatically at our synthetic biology laboratory.

Complex DNA Synthesis up to 400 bases

Complex synthetic oligos are purified by gel electrophoresis and analyzed by analytical gel electrophoresis and mass spectrometry.

Example: 190 mers DNA Synthesis

Sequence: GCA GCT TGG TTA CCA GAA CGA TAA CAA TAG TTT GCA GCT TGG TTA CCA GAA CGA TAA CAA TAG TTT GCA GCT TGG TTA CCA GAA CGA TAA CAA TAG TTT GGC AGC TTG GTT ACC AGA ACG ATA ACA ATA GTT TGC AGC TTG GTT ACC AGA ACG ATA ACA ATA GTT TGC AGC TTG GTT ACC AGA ACG ATA A

QC: LC/MS, ESI and PAGE Gel analysis

DNA Oligo Synthesis Case StudiesDNA Oligo Synthesis Case Studies DNA Oligo Synthesis Case Studies