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BNA qPCR primers and probes are dual labeled DNA or RNA probes designed for use in the 5' nuclease assays. Spiking oligonucleotide with BNA base in the probes or primers have increase sensitivity in real-time quantitative PCR assay. BNA Probes have increased sensitivity for distinguishing DNA base-pair mismatches, and are commonly used for SNP genotyping assays.
Bio-Synthesis is a sole supplier for BNA dual labeled oligonucleotide probe. All qPCR probes are available at a fixed price per oligonucleotide, within the following parameters:
If any help is needed qPCR primer design or the combination of dyes that should be used on a specific thermocycler, please do not hesitate to contact our scientific support that will be happy to help you.
Bridged Nucleic Acids (BNAs), a novel type of nuclei acid analogs that contains a six-member 2'-O,4'-aminoethylene bridge. This bridge-locked in 3' endo conformation which restricts the flexibility of the ribofuranoise ring and locks the structure into a rigid-bicyclic formation, allowing enhanced hybridization performance and biostability.
To overcome certain weakness of standard DNA probe chemistires, choose our range of Bridged Nucleic Acid (BNA) fluorescent probes, this powerful new generation sequence-specific real-time qPCR probes can be use in various PCR probe design:
Learn more about Bridged Nucleic Acids (BNAs)
All our BNA modified oligonucleotides undergo vigorous process of monitoring and strict quality control. Length and labeling are systematically controlled by PAGE, HPLC or MALDI-TOF mass spectrometry analysis. Quantity is systematically determined and validated by UV Absorbance at 260 nm. Fluorescent probes are systematically quality-checked by analytical ie-HPLC.
Single purification when labeling at time of the synthesis using phosphoramidite chemistry. Some labeling such as Texas Red, Alexa Flour or ATTO dye series must be labeld post-synthetically by covalently attach a primary amine modified oligonculeotide with dye via NHS ester chemistry. In this case, dual HPLC purified is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
BNA LightCycler FRET Probes can contain up to 6 BNA bases
BNA Molecular beacon can contain up to 8 BNA bases
Contact us for any dyes and quencher combinations not listed below.
Every oligo is quality checked by MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE).
Fully deprotected and desalted. Purified by PAGE or RP-HPLC
Delivered as dried-down product in opaque tubes
Turn-around time is dependent upon successful QC validation and does not include shipping time.
See information on our oligos: storage recommendations
Shipped by mail or express delivery, dry, in individual, opaque tubes
Oligonucleotides are delivered with an OligonucleotideTechnical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, length, extinction coefficient and purification data.
Additional services may increase turn-around time
Please contact your local Bio-Synthesis representative
On-line (contact us), by email (firstname.lastname@example.org) or fax