BNA Enhanced Real-time qPCR Primers and Probes
Bridged Nucleic Acids (BNAs), a novel type of nuclei acid analogs that contains a six-member 2'-O,4'-aminoethylene bridge. This bridge-locked in 3' endo conformation which restricts the flexibility of the ribofuranoise ring and locks the structure into a rigid-bicyclic formation, allowing enhanced hybridization performance and biostability.
Feature of BNA fluorescent probes
- Higher Tm by BNA modification increases thermal stability and hybridization specificity
- More accurate gene quantitation and allelic discrimination improve SNP detection assays
- Shorter NBA probes enable easier and more flexible probe designs for problematic target sequences
- Compatible with real-time PCR platforms and end-point analytical detection instruments - no additional investment on instrumentation is necessary
- Compatible with standard DNA and RNA phosphoramdite chemistry - allows the use of the same synthesis and modification protocols as DNA and RNA bases
To overcome certain weakness of standard DNA probe chemistires, choose our range of Bridged Nucleic Acid (BNA) fluorescent probes, this powerful new generation sequence-specific real-time qPCR probes can be use in various PCR probe design:
- BNA LightCycler FRET Probes
- BNA dual-labeled fluorogenic probes
- BNA molecular Beacon
Learn more about Bridged Nucleic Acids (BNAs)
Specifications of BNA real-time qPCR fluorescednt Probes
Quality Control:
All our BNA modified oligonucleotides undergo vigorous monitoring and strict quality control. Length and labeling is systematically controlled by PAGE, HPLC or MALDI-TOF mass spectrometry analysis. Quantity is determined and validated by UV Absorbance at 260 nm. Fluorescent probes are systematically quality-checked by analytical ie-HPLC.
Purification
Single purification when labeling at time of the synthesis using phosphoramidite chemistry. Some labeling such as Texas Red, Alexa Flour or ATTO dye series must be labeld post-synthetically by covalently attach a primary amine modified oligonculeotide with dye via NHS ester chemistry. In this case, dual HPLC purified is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Bases Assignment
DNA/BNA Chimeras:
- BNA base (+A, +C, +T, +G) (+ denotes the BNA bases)
- DNA (A,T,C,G)
- RNA (rA, rT, rC, rG)
BNA LightCycler FRET Probes can contain up to 6 BNA bases
BNA Molecular beacon can contain up to 8 BNA bases
Contact us for any dyes and quencher combinations not listed below.
Specifications
Quality control:
Every oligo is quality checked by MALDI-TOF mass spectrometry, electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and/or polyacrylamide gel electrophoresis (PAGE).
Purification
Fully deprotected and desalted. Purified by PAGE or RP-HPLC
Length
|
Length |
| LightCycler probes |
15 to 40 mers (optimal length: 20 to 30 mers) |
| Dual-labeled fluorogenic probes: |
15 to 40 mers |
| Molecular Beacons |
15 to 40 mers |
| Scorpions probes |
30 to 60 mers (uni-probe) |
| 15 to 45 mers (bi-probe) |
| Plexor |
up to 30 mers |
Backbone
Phosphodiester bond
Format
Delivered as dried-down product in opaque tubes
Turn-around time:
|
Turn-around time |
| LightCycler probes |
4 to 5 working days |
| Dual-labeled fluorogenic probes: |
5 to 6 working days |
| Molecular Beacons |
5 to 6 working days |
| Scorpions probes |
7 to 10 working days |
Turn-around time is dependent upon successful QC validation and does not include shipping time.
Storage and stability:
See information on our oligos: storage recommendations
Shipment:
Shipped by mail or express delivery, dry, in individual, opaque tubes
Technical Documentation:
Oligonucleotides are delivered with an OligonucleotideTechnical Data Sheet, which includes oligonucleotide name, sequence, concentration, precise quantity in OD and nmols, Tm, MW, length, extinction coefficient and purification data.
Additional Services:
- Probe design services
- High-throughput screening formats (microplates, etc)
- Aliquoting
Additional services may increase turn-around time
Pricing:
Please contact your local Bio-Synthesis representative
Ordering:
On-line (contact us), by email (info@biosyn.com) or fax