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Protein Expression Services

At Bio-Synthesis, we can guide you through a full protein expression development cycle. Our Protein Expression Services are uniquely positioned to help you by providing streamlined Gene to Protein Expression Services. Backed by years of experience in molecular biology, protein biochemistry and cutting-edge technology, our scientists can express, purify, and characterize proteins that match your exact needs.

Protein Expression Services

Our Advantage

  • Free Condon optimization services
  • Multiple protein expression system
  • Gene cloning and protein expression vector construction
  • Flexible protein purification system
  • Large-scale production
  • Quality control test development and validation
  • Finished product formulation and stability analysis
  • Technology transfer
  • 100% confidentiality

Protein Expression Services

  • Recombinant antigens for vaccine development
  • Proteins for use in diagnostic kits
  • Proteins for structure studies
  • Proteins for functional studies and enzymatic assays
  • Protein-protein and ligand interactions studies
  • Protein microarrays
  • Protein libraries
  • Biomarker development
  • Drug screening target

For additional information, please contact us or send an email to info@biosyn.com

Service Bacterial cell Insect cells Mammalian cell
Construct Generation and Subcloning
Transfection and Transformation
Clone verification by Sequencing and Restriction Analysis
Cell Line Propagation
Optimization of Expression in Multiple Strains
Cell Line and Medium Optimization
Growth & Expression Condition Optimization
Large Scale Expression
Purification & Characterization

Custom Recombinant Protein Purification

At Bio-Synthesis, we custom design protein purification strategies for each individual protein to ensure a high recovery rate. This enables us to purify most untagged recombinant proteins within three steps: 1. Capture, 2. Intermediate-step, 3. Polish. For tagged proteins, our two-step protein purification method can achieve greater than 95% purity in most cases.

  • Multiple protein purification systems in a temperature controlled environment
  • Extensive collection of chromatography media
  • Innovative configurations of chromatography columns custom tailored to each protein
  • Vigorous quality assurance programs to ensure the required quality and reproducibility
  • Production capacity of up to tens of grams
  • Greater than 98% purity

Custom Antibody Services

Additional Services

    Molecular Biology Services

    Bio-Synthesis provides a full range of molecular biology services with fast delivery at affordable prices.

    • Gene synthesis (with or without codon optimization)
    • Gene cloning using our NovoRec seamless cloning
    • Mutagenesis
    • DNA plasmid preparation and large scale production

    Isotope Labeling (E. coli system only)

    Uniform isotope labeling is a key tool for NMR studies on recombinant proteins and their ligand interactions for pharmaceutical interest. A minimal medium is required for specific labeling in E. coli systems in order to control the nutrients found in the broth. However, a typical minimal medium (such as M9) cannot support the same level of growth as a richer medium and thus limits the amount of protein produced by limiting the cell density of the culture. This constraint results in a high cost for isotope labeling. With years of experience in bacterial culture and protein expression, our scientists at Bio-Synthesis have developed a fortified minimal medium which supports growth and protein expression that is comparable to the standard medium. This proprietary medium drastically lowers cost, providing a large amount of recombinant protein uniformly labeled with a stable isotope. We provide stable isotope labeling services in our E. coli expression system with 15N, 13C.

    Protein Refolding (E. coli system only)

    Production of foreign proteins in E. coli may result in the formation of protein aggregates, more commonly known as inclusion bodies. Specific empirical conditions are required to make inclusion bodies soluble and further refold denatured proteins. At Bio-Synthesis, our scientists devised a proprietary refolding system that is comprised of a variety of refolding buffers optimized with various parameters. Using this optimized system, we have successfully refolded more than 300 proteins, which has earned us a spot as a leader in the field.

    Bio-Synthesis protein refolding services include the following steps:

    • Protein expression and inclusion body preparation (optional)
    • Solubilize inclusion body to maximum monomer status
    • Small-scale refolding experiments to determine the best refolding conditions by activity assay
    • Small-scale refolding
    • Large scale refolding (optional)

    Process Development

    Our process development ensures that protein production is scalable, reproducible, and cost effective. It is furthermore effective in bridging the gap between small and large-scale production. Our process is optimized to significantly improve the recovery rate and the bioactivity of your recombinant Proteins. Let us help you:

    • Develop your protein production process from inception
    • Update your current process to yield optimal results

    Protein Characterization

    Bio-Synthesis employs a broad range of methods to characterize our protein products:

    • Electrophoresis
    • N-terminal amino acid sequencing
    • Mass spectrometry
    • Bioactivity assay
    • Western blotting
    • ELISA
    • HPLC
    • Endotoxin test

    Protein Crystallization

    We have developed our protein crystallization services based on our solid protein expression and purification skills.
    We offer:

    • Crystal screening using high throughput kit
    • Crystal optimization with various parameters
    • Co-crystallization with ligand compound
    • X-ray analysis