Bridged Nucleic Acids (BNA)
At Bio-Synthesis, we can guide you through a full protein expression development cycle. Our Protein Expression Services are uniquely positioned to help you by providing streamlined Gene to Protein Expression Services. Backed by years of experience in molecular biology, protein biochemistry and cutting-edge technology, our scientists can express, purify, and characterize proteins that match your exact needs.
At Bio-Synthesis, we custom design protein purification strategies for each individual protein to ensure a high recovery rate. This enables us to purify most untagged recombinant proteins within three steps: 1. Capture, 2. Intermediate-step, 3. Polish. For tagged proteins, our two-step protein purification method can achieve greater than 95% purity in most cases.
Bio-Synthesis provides a full range of molecular biology services with fast delivery at affordable prices.
Uniform isotope labeling is a key tool for NMR studies on recombinant proteins and their ligand interactions for pharmaceutical interest. A minimal medium is required for specific labeling in
E. coli systems in order to control the nutrients found in the broth. However, a typical minimal medium (such as M9) cannot support the same level of growth as a richer medium and thus limits the amount of protein produced by limiting the cell density of the culture. This constraint results in a high cost for isotope labeling. With years of experience in bacterial culture and protein expression, our scientists at Bio-Synthesis have developed a fortified minimal medium which supports growth and protein expression that is comparable to the standard medium. This proprietary medium drastically lowers cost, providing a large amount of recombinant protein uniformly labeled with a stable isotope. We provide stable isotope labeling services in our E. coli expression system with 15N, 13C.
Production of foreign proteins in E. coli may result in the formation of protein aggregates, more commonly known as inclusion bodies. Specific empirical conditions are required to make inclusion bodies soluble and further refold denatured proteins. At Bio-Synthesis, our scientists devised a proprietary refolding system that is comprised of a variety of refolding buffers optimized with various parameters. Using this optimized system, we have successfully refolded more than 300 proteins, which has earned us a spot as a leader in the field.
Bio-Synthesis protein refolding services include the following steps:
Our process development ensures that protein production is scalable, reproducible, and cost effective. It is furthermore effective in bridging the gap between small and large-scale production. Our process is optimized to significantly improve the recovery rate and the bioactivity of your recombinant Proteins.
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Bio-Synthesis employs a broad range of methods to characterize our protein products:
We have developed our protein crystallization services based on our solid protein expression and purification skills.