Bridged Nucleic Acids (BNA)
Long RNA oligo synthesis is used in the production of Synthetic long RNA oligos for a variety of applications ranging from understanding the function of newly discovered genes to gene analysis. Long RNA oligos function as positive controls for sensitive molecular diagnostic tools and the development of medical therapeutics. Bio-Synthesis offers chemical long RNA synthesis (up to 300 bases) or Long RNA transcripts up to kilobases from a DNA template . These ready-to-use custom RNA fragments include custom mRNA, tRNA, or other long noncoding RNA (lncRNA). Bio-Synthesis also provides RNA oligo services for a wide range of modifications and labels.
Bio-Synthesis has optimized the chemical synthesis of long RNA. Every long RNA oligonucleotide manufactured at Bio-Synthesis undergoes quality control analysis by PAGE, HPLC and/or MS. All RNA oligonucleotides are packaged using our proprietary RNaseSAFE™ service, in which RNA oligos are packaged in RNase and DNase free matrix tubes to prevent degradation during shipping and storage and safeguard the biological activity of RNA oligos.
For additional information, please contact us or send an email to email@example.com
While there has been significant demand for solid phase synthesis of long RNA fragments such as miRNA, shRNA in gene structure and functional studies, the ability to synthesize longer RNA oligos is highly sequence-dependent. This is due to the potential for secondary structure formation and the inherent difficulty for chemical synthesis with additional 2'-OH reactive group of RNA which introduces considerable complexity to RNA synthesis. Therefore,an effective RNA oligo synthesis method for longer RNA is an important basic technology for further advancement of RNA related research.
Bio-Synthesis has met the challenge of synthesizing thousands of long RNA Oligonucleotides of up to 300 bases or RNA transcripts from a DNA template. We use the best reagents available and proprietary methods to optimized synthesis protocols in a state-of-art RNA synthesis facility. Each RNA oligo is meticulously monitored during synthesis and undergoes stringent quality assurance and quality control standards. The final product is identified by LC-MS Electrospray, sequencing or other custom analytical methods. Purity is analyzed by high throughput capillary gel or PAGE electrophoresis.
Long RNA oligos are used in variety of molecular biology applications:
All Bio-Synthesis oligos undergo desalting and deprotection at no additional charge.We strongly recommend that oligonucleotides longer than 50 bases be purified by RNase free poly acrylamide gel electrophoresis (PAGE). If you are unsure of the purification level you need for your research, see our technical bulletin or contact us for assistance.
Yield will vary based upon base composition, purification, oligo length, scale and
modifications. That's why we have a set a guaranteed quantity.
Oligos are checked for length, purity, and composition. Long RNA oligos are quality checked by PAGE, ESI-MS, and/or CE. Our quality control capabilities include MALDI-TOF mass spectrometry, Electrospray ionization mass spectrometry (ESI-MS), capillary electrophoresis (CE), and polyacrylamide gel electrophoresis (PAGE). If you report an oligo problem within 30 days of order receipt, we will gladly remake your oligo free of charge or provide a credit for the purchase amount.
All orders are shipped with a Quality Assurance document that includes measured yields, melting temperature, MW and µg/OD with every oligo.
Bio-Synthesis provides synthesis of oligo ultramer and modification with flexible synthesis scales for your individual research needs. The ability to synthesize RNA oligos is critical to many laboratory techniques. Radiolabeled and nonisotopically labeled RNA probes can be used in blot hybridization and nuclease protection assays. Modified
RNA can be used for various biochemical and molecular biology studies. RNA can also be used as a control for gene expression analysis (microinjection, infection with viral transcripts, in vitro translation), structure analysis (protein-RNA binding), and RNA -based diagnostic testing and drug development.
Contact us for large scale synthesis.
Contact us for modification not
listed above, in most cases we can help!