Enhanced Diagnostic Tools
We are pleased to offer products based on Epoch’s Redmond Red™, Yakima
Yellow™ and Gig Harbor Green™ fluorophores and Eclipse™ non-fluorescent
quencher. The two fluorescent dyes, Yakima Yellow and Redmond Red, are available
to be labeled at 5' or 3' end of an oligo. Yakima Yellow has an absorbance maximum
at 530 nm and emission maximum at 549 nm, while Redmond Red’s absorbance and
emission maxima are at 579 nm and 595 nm, respectively. Gig Harbor Green and 6-FAM
are based on the same fluorescein core structure but Gig Harbor Green is 15-20%
brighter than FAM. The Eclipse quencher from Epoch solves most of the problems inherent
in the synthesis of molecular beacon and FRET probes. The Eclipse molecule is highly
stable and can be used safely in all common oligo deprotection schemes. The absorbance
maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl. In addition,
the structure of the Eclipse Quencher is substantially more electron deficient than
that of dabcyl and this leads to better quenching over a wider range of dyes, especially
those with emission maxima at longer wavelengths (red shifted) such as Redmond Red
and Cy5. In addition, with an absorption range from 390 nm to 625 nm, the Eclipse
Quencher is capable of effective performance in a wide range of colored FRET probes.
Contact your Account Representative for pricing on Epoch dye and Quenchers labeled
oligonucleotides. For additional information, please Contact us.
Price/labeling, purification and synthesis is an additional charge
A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo.Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.