Enhanced Diagnostic Tools
All of our dyes can be attached post synthetically to the 5'- terminus of an oligonucleotide
via an amino- or thiol- linker with a 6-Carbon spacer arm. This method is available
for JOE .
Another possibility for labeling an oligonucleotide at the 5' or internal terminus
is to couple the dye directly during synthesis via its phosphoramidite. This method
is available for 6-FAM, Fluorescein, Fluorescein dT, 6-Fluorescein, HEX, TET .
Post synthetic labeling of oligonucleotides at internal sites of the sequence is
possible by substituting any thymidine with 5-C6-Amino-2'-deoxythymidine. All dyes
available for 5'- labeling can also be attached internally.
It is also possible to label the 3'- end of oligonucleotides post synthetically
via an amino link. This method is available for all dyes. For the 3'- labeling with
fluoresce in , BSI has special solid supports [CPGs] with the dyes already attached.
This is very useful for high quality production of labeled probes.
Contact your Account Representative for pricing on Epoch dye and Quenchers labeled
oligonucleotides. For additional information, please Contact us.
Price/labeling, purification and synthesis is an additional charge
* Product Note - This compound requires amino- or thiol- linker with a 6-Carbon
spacer arm or by substituting any thymidine with 5-C6-Amino-2'-deoxythymidine for
internal labeling, a double HPLC purification is required.
A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo.
Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.