Enhanced Diagnostic Tools
Synthetic or enzymatic mRNAs have been used to study protein-mediated mRNA recognition, mRNA export and translation, as well as mutation effects in protein-coding genes. More recently, mRNA-based drugs have been developped for cancer immunotherapy and Prophylactic Vaccines. The limitation of chemically synthesized mRNA longmer (>200 mer) at large quantity continues to be a synthetic bottleneck. In contrast, template directed mRNA synthesis by in vitro transcription up to thousands of bases is highly feasible.
Bio-Synthesis has provided thousands of custom mRNA synthesis from a plasmid-based template by in vitro transcription. mRNA can be generated by DNA templates provided by you or we can provide complete service from the ground up at Bio-Synthesis. This complete service allows the researcher to tailor synthesis and introduce modifications that produce a transcript that is optimal for downstream applications.
Please contact us for more information on mRNA
We provide mCAP (m7G(5')ppp(5')G capping for all our standard mRNA transcripts. We also provide ARCA, anti-Reverse Cap Analogs (3' O-Me-M7G(5')ppp(5')G) as an option. ARCA can only be inserted in the proper orientation which leads to higher translation levels.
We incorporate polyadenylation by template driven enzymatic methods. These methods produce mRNA with a >150 nts long poly(A) tail which is often used to enhance translation efficiency.
Yes, phosphatase treatment is part of our standard mRNA transcription procedure. This triphosphates containing mRNA is a result of incomplete capping procedure. In some cases, it is necessary to remove 5'-triphosphate to reduce possible innate immune responses in mammalian cells.
When ordering mRNA transcript services, please provide information such as mRNA sequence, desired 5' and 3'-UTR sequence. You may also provide additional specifications for modified bases such as s5-meC, pseudouridine (ψ) or any others.
For mRNA plasmid templates provided to Bio-Synthesis, please supply 5 ug plasmid DNA and information such as: