ATTO 647N Fluorescent Dye Oligonucleotide Labeling

Bio-Synthesis offers ATTO 647N fluorescent dye oligonucleotide labeling services. ATTO 647N is a fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high photostability with good water solubility.

ATTO 647N is a cationic  dye, it consists of a mixture of two isomers with identical absorption and fluorescence properties.  The fluorescence is excited most efficiently in the range 625- 660 nm, a suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.  They dye can be use as an alternative dye for Cy5, Alexa flour 647 or DY649. 

• Replacement dye for CY5, Alexa 647 and DY649
• Extraordinarily high fluorescence yield '
• High Thermo and photo-stability
• Excellent ozone resistance
• Moderate hydrophilic
• Cationic dye, mixture of two isomers

ATTO 647N dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification,  after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.

Contact us for ATTO 647N fluorescent dye oligonucleotide labeling services.