Enhanced Diagnostic Tools
Pseudo-complementary base modified oligonucleotides, originally referred to as selectively binding complementary oligonucleotides, are important tools for the study of duplex structures in RNA and DNA double strands. Oligonucleotides modified with non-standard selective or pseudo-complementary bases show significantly reduced hybridization affinities for the formation of duplexes with each other due the presence of chemical modification. However, many pseudo-complementary modified oligonucleotides can form strong base pairs with natural DNA or RNA target sequences. Consequently, pseudo-complementary nucleic acids show diminished intra-molecular and inter-molecular secondary structures, but can readily hybridize to unmodified nucleic acids. The differential hybridization properties of pseudo-complementary nucleic acids allow for sequence-specific targeting of duplex DNA using various double duplex invasion strategies. Examples are oligonucleotides containing palindromic (self-complementary) sequence stretches that allow the design of hybridization probes that form hairpins in RNA targets.
Bio-Synthesis offers several pseudo-complementary bases to be incorporated at the 5', 3' or internal position of an oligonucleotide.
Contact Bio-Synthesis for Pseudo-complementary nucleobase oligo modification.
All oligonucleotide synthesized at Bio-Synthesis is quality check by using either MALDI-TOF mass spectrometry, analytical HPLC,or polyacrylamide gel electrophoresis (PAGE). Final yields are determined using UV absorbance at OD260 In addition, we perform custom formulation and quality control tailored to meet your specific requirements.
Quality Control: Analytical HPLC, Gel eletrophoresis, and MALDI-TOF Mass Spectrometry
Delivery times: 5-7 Working days
Shipping conditions: Room temperature
Storage conditions: -20 oC to -70 oC
Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.