Enhanced Diagnostic Tools
Bio-Synthesis supplies Custom RNA Oligo Synthesis services for a wide range of labels, modifications, scales and purities. We provide RNA synthesis for long RNA oligos up to 300 bases.
RNA oligos between 80-300 bases are synthesized using the latest nucleic acid technology and are purified using RNase free PAGE electrophoresis combined with HPLC. Our RNA oligonucleotide synthesis services are provided with mass spectrometric analysis. Purified RNA oligonucleotides come with stringent analytical HPLC or gel electrophoresis to determine final purity.
RNA oligonucleotides are processed using our RNaseSAFETM service, in which RNA oligos are packaged in RNase free matrix tubes to prevent degradation during shipping and storage. This standard service helps safeguard the biological activity of RNA oligos.
For additional information on RNA synthesis services such as RNAi, siRNA, shRNA, mRNA, please contact us or send an email to firstname.lastname@example.org.
RNA synthesis and deprotection is performed using proprietary parallel synthesis and deprotection technologies. This advanced technology enables us to synthesize oligos with higher coupling efficiency and fast deprotection, resulting in quick turnaround time.
Available Lengths: RNA oligos are available from 10-80 bases for 100 nmole, 5-90 bases for 250 nmole and 1 µmole, and 5-50 bases for 5 µmole and 10 µmole
for sequences >90 bases please contact us or request a quote.
* Chemical Long RNA Synthesis up to 250 bases
* Long RNA Transcripts >1000 bases
We provide RNA oligonucleotide synthesis in quantities ranging from multi-mg to several grams. RNA analogs and chimeras are available from 10 micromoles up to 10 grams! Contact us for more information.
siRNA arrays are available in 96-Well-Format with oligos 10-20 bases at 1 ODU deprotected and desalted dry or in liquid format.
Bio-Synthesis is able to chemically synthesizes high quality long RNA of >100 nt. The ability to synthesize longer RNA oligos is highly sequence-dependent due to the potential for secondary structure formation and the inherent difficulty for chemical synthesis of certain sequences. Reduced yield is possible as oligo length increases. We also offer enzymatic RNA synthesis by transcription up to thousands of bases in length using DNA template.
Please contact us to learn more about Chemical Long RNA or Enzymatic Long RNA Synthesis.
GCC ACU UAA ACG ACC UGG AUG UUG CUU UAC UGA AAC UAA AGA AAG UGC AGU UUC CAU UUG GUG GUU AUG GU
Length: 71 nt Calculated MW: 22765.65Detected MW: 22766.5
Sequence: GAAUACUGGAGGGUCAAUACACGUUGGAGUGGCGGACGGGCCGAAGGCGGCGUGAGCGGCGAAGGGAUGGUGAGGUCGGAGUGGUGAGGCGGAGUCGCGUGGUGAGCGAGCGCGGUGUGGCGUGAGAGGCGUUCUGCUGGUCAGUAACCAGGU 3'
Length: 153 nt Calculated MW: 22765.65 Detected MW: 22766.5
Column: Phenomenex, Clarity 3u, oligo-RP, 150*2.00 mm, 3 micron Solvent: A 20 mM TEAA, pH 8.0; B: Acetonitrile
5% denaturing PAGE, EtBr staining Lane 1: 153 nt RNA Lane 2: RNA Marker
Sequence: 1429 nt
Calculated Mass: 460256.4
Size Analysis: 5% denatured PAGE, EtBr stainingCalculated size: 1429 nt Observed size: 1429 nt
5% denaturing PAGE picture, EtBr staining Lane 1: RNA markers Lane 2: 1429 nt RNA