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The interactions of biotin and biotin binding proteins have been a useful tool in protein and nucleic detection and purification. A variety of molecules, including lectins, proteins, and antibodies, can be biotinylated and used as primary probes for detection with avidin or streptavidin "secondary" probes, or other detection reagents for use in biological assays.
Conjugates of high-quality and reproducible secondary antibodies are essential for consistent experimental results. Bio-Synthesis provides Custom Secondary Antibodies
Detection Probes using state-of-the-art conjugation chemistries and purification
methods with the ability to label microgram to milligram amounts of your antibody in a short amount of time.
We offer custom conjugation of these secondary probes by request:
To obtain further Information, please contact us.
The following is a list of our currently available custom antibody detection probe services. Should you not find a service that suits your needs, we will find a solution for you!
Streptavidin, avidin and NeutrAvidin biotin-binding proteins each bind four biotins
per molecule with high affinity and specificity. Their multiple binding sites permit
a number of techniques in which unlabeled avidin, streptavidin or NeutrAvidin biotin-binding
protein can be used to bridge two biotinylated reagents. This bridging method, which
is commonly used to link a biotinylated probe to a biotinylated enzyme in enzyme-linked
immunohistochemical applications, often eliminates background problems that
can occur when using direct avidin or streptavidin-enzyme conjugates. The table
below is a comparison of each binding protein we offer.
We offer three different kinds of biotin-binding proteins: