Enhanced Diagnostic Tools
The interactions of biotin and biotin binding proteins have been a useful tool in
protein and nucleic detection and purification. A variety of molecules, including
lectins, proteins, and antibodies, can be biotinylated and used as primary probes
for detection with avidin or streptavidin "secondary" probes, or other detection
reagents for use in biological assays.
Conjugates of high-quality and reproducible secondary antibodies are essential for
consistent experimental results. Bio-Synthesis provides Custom Secondary Antibodies
Detection Probes using state-of-the-art conjugation chemistries and purification
methods with the ability to label microgram to milligram amounts of your antibody
in a short amount of time.
We offer custom conjugation of these secondary probes by request:
To obtain further Information, please contact us.
The following is a list of our currently available custom antibody detection probe
services. Should you not find a service that suits your needs, we will find a solution
Streptavidin, avidin and NeutrAvidin biotin-binding proteins each bind four biotins
per molecule with high affinity and specificity. Their multiple binding sites permit
a number of techniques in which unlabeled avidin, streptavidin or NeutrAvidin biotin-binding
protein can be used to bridge two biotinylated reagents. This bridging method, which
is commonly used to link a biotinylated probe to a biotinylated enzyme in enzyme-linked
immunohistochemical applications, often eliminates background problems that
can occur when using direct avidin or streptavidin-enzyme conjugates. The table
below is a comparison of each binding protein we offer.
We offer three different kinds of biotin-binding proteins: