Enhanced Diagnostic Tools
Synthetic siRNA and shRNA constructs are two of the numerous options available.
BSI provides the custom synthesis of sequence-specific siRNA for direct transfection,
as well as the routine synthesis of shRNA oligos, ~60 bases long purified DNA oligos
to be cloned to form shRNA constructs . Our siRNAs (small interfering RNAs) are
double-stranded RNA molecules, made of 19 RNA nucleotides with two nucleotides (usually
DNA bases) 3' overhangs. They are used to knockdown a specific gene of interest
for identifying gene function, elucidating pathways and screening for potential
new drug targets.
Custom siRNA is available with a variety of fluorescent labels and other modifications.
Alexa Fluor dyes provide superior performance and can be directly substituted for
other common dyes. Other fluorescent dyes available include fluorescein, rhodamine,
Cy3, and Cy5. Custom siRNA may be labeled at either the 3' or the 5' end of the
sense strand, and labels do not affect the biological activity of the siRNA. As
well as fluorescent labels, many other backbone and base modifications are available
including amino linkers, thio linkers, biotin, dabcyl and phosphate modifications. View the full range of modification
options Special modifications are also available on request;
please contact our RNA oligo
All siRNA oligos are provided as a lyophilized pellet to ensure stability during
Bio-Synthesis provides siRNA by using proprietary synthesis and purification processes
with final products that are ready-to-use. There is no need for further time-consuming
and expensive HPLC or PAGE purification. The high-throughput synthesis and purification
processes enable fast turnaround times and high yields. Each duplex siRNA synthesis
undergoes stringent quality control including MALDI-TOF mass spectrometry analysis
that verifies identity of the component strands and analytical HPLC QC that verifies
purity of the duplex itself, a level of QC unparalleled in the industry. Our high
performance grade siRNA is economically priced allowing the use of highly pure siRNA
in all routine RNAi experiments. For very sensitive applications, custom synthesis
of HPLC-purified siRNA is also available upon request. Read more about siRNA synthesis
platform and technology.