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Sample Preparation Guidelines

Sample Preparation Guidelines
Array Processing Services

Target Sample Submission Guidelines

Total RNA Sample:

  1. Quality of total RNA: All RNA samples should meet assay quality standards to ensure the highest quality RNA is hybridized to the gene expression arrays. User should run the initial total RNA on an agarose gel or bioanalyzer to examine the ribosomal RNA bands. 260/280 absorbance readings should be measured for total RNA. Acceptable 260/280 ratios fall in the range of 1.8 to 2.1.

  2. Amount of total RNA: Depending on different platform to use, different amount is required. Generally, 1-5 total RNA is needed. The RNA concentration should be greater than 0.1ug/ul (add RNase-free water to adjust same concentration).

  3. Please submit a copy of the photograph of your total RNA on the agarose gel electrophoresis or bioanalyzer printout, and 260/280 and 260/230 absorbance reading together with the sample(s).

Genomic DNA Sample:

  1. Quality of DNA: User should run the initial DNA on an agarose gel or bioanalyzer to examine the ribosomal RNA bands. 260/280 absorbance readings should be measured for genomic DNA. Acceptable 260/280 ratios fall in the range of 1.6 to 1.8.

  2. Amount of DNA: 1.0 μg DNA is required. The DNA concentration should be 50ng/μl. DNA should be dissolved in DNase-free water or low EDTA TE buffer.

  3. Please submit a copy of the photograph of your DNA on the agarose gel electrophoresis or bioanalyzer printout, and 260/280 and 260/230 absorbance reading together with the sample(s).

Shipping:

Overnight shipment using a commercial carrier(FedEx or UPS, etc) is required.

  1. For tissues and cells: Keep in dry ice for frozen tissues and cell pellets. Never let it thaw, so make sure there is enough dry ice. For RNAlater kept tissues or cells, use ice pads instead.

  2. For total RNA and Genomic DNA: Ship with dry ice.

Array Printing Sample Submission Guidelines

DNA (cDNA or oligos):

  1. Quality of oligos: 260/280 absorbance readings should be measured using a spectrophotometer. Acceptable 260/280 ratios fall in the range of 1.8 to 2.1.

  2. Amount of oligos: At least 50ul in DNase-free water is needed. The DNA concentration should be between 0.1 and 0.5ug/ul (add DNase-free water to adjust each sample to same concentration).

  3. Samples should be kept in 96-well microtiter plates.

  4. A sample sheet with sample names should be included with the shipment. Also please specify number of arrays and number of replicated spots on the arrays.

Protein/Antibodies:

  1. Proteins/Antibodies should be dissolved in printing buffer (1x PBS/0.05 BSA) to 0.5mg/ml or higher. At least 50 ul in volume is required.

  2. Samples should be kept in 96-well microtiter plates.

  3. A sample sheet with sample names should be included with the shipment. Also please specify number of arrays and number of replicated spots on the arrays.

Shipping:

Overnight shipment with ice pad using a commercial Carrier(FedEx or UPS, etc) is required.