Enhanced Diagnostic Tools
NEBcutter Restriction Analysis
NEBcutter is an on-line tool for restriction mapping nucleotide sequences that also features identification of non-overlapping ORFs.
Watcut Restriction Analysis
Alternative restriction enzyme analysis..
Convert DNA sequence data to it's Reverse Complement.
Raw Translation of DNA
Translate DNA sequence in all 6 possible frames.
Formatted DNA Sequence Translation
There are two sites you can use. The preferred one is located at www.justbio.com. You will need to register yourself with this site before you can use this tool and you must use Internet Explorer V5.5 (or better) or Netscape V6 (or better). This facility allows translation of DNA sequence in one, or more frames. The output is formatted as free text amino acid sequence as well as DNA sequence plus the aligned translation. This utility is a useful and fast way to format your sequence following an Open Reading Frame analysis.
Graphical Open Reading Frames Analysis at NIH
Graphically identify ORFs within DNA sequence data.
Identification of Prokaryotic Promoters
A neural network based program to find possible transcription promoters. From the Berkley Drosophila Genome Project
Screens DNA sequences for interspersed repeats and low complexity DNA sequences. Multiple sequences may me submitted in FASTA format
EINVERTED, PALINDROME and EQUICKTANDEM
Find inverted and tandem repeats in sequence data.
Codon usage table analysis
Java Script Oligo calculator (Netscape V2 or better).
Graphical Codon Usage Analyser
Differences in codon usage among organisms can lead to a variety of problems concerning heterologous gene expression. The GCUA tools display the codon bias in a graphical manner.