Cholesteryl TEG Modified Oligonculeotide
Bio-Synthesis offers cholesteryl TEG (15 atom triethylene glycol spacer) modified oligonucleotide synthesis services. Since oligonucleotides are predominantly hydrophilic, they tend to have difficulty permeating cell membranes. In order to improve cellular uptake, one strategy is to conjugate to oligonucleotides molecules that are non-toxic and hydrophobic. Cholesteryl conjugated oligonucleotides have been the subject of great interest in antisense and other studies. The addition of cholesterol has been shown to enhance the penetration of oligonucleotides into the cells1, probably due to lipophilic nature of the cholesterol molecule. It has been used as a transfection aid for antisense oligos and siRNAs, both in vitro and in vivo. 3’-Cholesterol containing oligonucleotides exhibited significant 3’-endonuclease resistance.2-4 Cholesterol is a very hydrophobic modification that is best purified using RP-HPLC.
Product Information
Cholesteryl TEG Modified Oligonculeotide
End Modifier, Antisense, siRNA, Cell Penetration
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- M.K. Bijsterbosch, et al., Nucleic Acids Res., 2000, 28, 2717-2725.
- M.K. Bijsterbosch, et al., J. Pharmacol. Exp. Ther., 2002, 302, 619-626.
- M. Manoharan, Antisense Nucleic Acid Drug Dev, 2002, 12, 103-28.
- M. Manoharan, Curr Opin Chem Biol, 2004, 8, 570-9.
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