Custom DNA Synthesis--"FASTEST services in the industry"
Oregon Green® Fluorophores Labeling
There are different possibilities for labeling fluorescein dye to an oligonucleotide:
Oregon Green, an alternative to fluorescein with greater photostability and higher
fluorescence }
This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by
substituting any thymidine with 5-C6-Amino-2'-deoxythymidine for internal labeling,
a double HPLC purification is required.
Contact your Account Representative for pricing on rhodamine dye labeled oligonucleotides.
For additional information, please Contact us.
Price/labeling, purification and synthesis is an additional charge
| Dyes
|
Scales |
Label position
|
| 5'
|
Int
|
3'
|
|
Oregon Green® 488, 5- Isomer
|
250 nmol
|
$200
|
$256
|
$225
|
|
1.0 µmol
|
$280
|
$334
|
$300
|
|
Oregon Green® 488, 6- Isomer
|
250 nmol
|
$200
|
$256
|
$225
|
|
1.0 µmol
|
$280
|
$334
|
$300
|
|
Oregon Green® 514
|
250 nmol
|
$200
|
$256
|
$225
|
|
1.0 µmol
|
$280
|
$334
|
$300
|
Purification
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite
modifiers. However, additional purification is strongly recommended for modifiers
that require NHS-ester chemistry to conjugate a dye through an amine linker such
as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60
% less than a standard oligo. Please see our yield chart for details.
Quality Controls
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF
mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields
are determined using UV absorbance at OD260 In addition, we perform QC
methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please Contact us.
The most trusted name in Quality Custom Synthesis