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DNA Modifications
Spiked Oligo

Spiked Oligos

Spiked bases refer to non-equimolar mixtures of bases. Spiked oligonucleotides are useful when a determined ratio of degenerated bases is required at precise positions within the sequence. This is generally required when one wants to use the preferential genetic codons rather than to degenerate the wobble position with a statistical mix of dNTP.

Spiking Bases Qty. 5'-Mod Internal 3' -mod
200 nmol 15 OD Inquire Inquire Inquire
1.0 µmol 35 OD Inquire Inquire Inquire

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

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