Bio-Synthesis offers affinity ligand oligonucleotide synthesis and modification that allows researchers to use as probes in solution, membrane, or bead-based assay systems. These affinity ligands have high binding affinity toward a specific moiety or they can be used to generate antibodies. Oligo affinity ligand modifications include: biotin (ligand)-streptavidin (moiety), digoxigenin (ligand)-anti-DIG-antibody and dinitrophenol (ligand)-anti-DNP-antibody. In the appropriate capture medium, these ligand modified oligonucleotides can be used to capture and purification of the oligo by affinity chromatography.

These ligands can be incorporated at any position within an oligonucleotide post-synthetically or at time of synthesis. An oligonucleotide modified with a primary amine group is used to covalently attach dye with oligonucleotide via NHS ester chemistry; other cross-linking chemistries are also available depending on particular project specifications, Dual HPLC purification is highly recommended for post-synthetic conjugation.

contact us for affinity ligand modified oligonucleotide synthesis services.

Our Advantages

  • Wide selection of modified RNA,DNA bases beyond 5-Mthyl C
  • Flexibility in oligo length and synthesis scales
  • Oligonucleotides are delivered as deprotected, desalted and/or purified
  • Quality checked by MALDI-TOF/LC-MS, analytical HPLC, PAGE
  • Specialize in producing newly-discovered and difficult -to-make modified bases
Product Modification Code 5 Prime Internal 3 Prime
Biotin [5 Biotin] Y Y Y
Biotin dT [5 Biotin-dT] Y Y Y
Biotin TEG (15 atom triethylene glycol spacer) [Biotin-TEG] Y Y Y
Desthiobiotin [DesthioBiotin-TEG] Y Y Y
Branch Biotin [Biotin-Branch] Y Y Y
Digoxigenin [Dig-NHS] Y Y Y
2,4-dinitrophenyl, DNP [DNP] Y Y Y
2,4-dinitrophenyl TEG, DNP-TEG [DNP-TEG] Y Y Y
Photocleavable PC Biotin [PC-Biotin] Y Y Y
NTA (Nitrilotriacetate) [NTA] Y Y Y
PseudoUridine Ribo [Psd-rU] Y Y Y

Quality Control: Analytical HPLC, Gel eletrophoresis, and  MALDI-TOF Mass Spectrometry

Delivery times: 5-7 Working days

Packaging:    Dried

Shipping conditions: Room temperature

Storage conditions: -20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please Call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."