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Clinical Specimen DNA Identification

Tissue DNA Identification Services

Pathological Specimen DNA identification to determine relationship between patient specimens, tissues, or tissue fragment for quality assurance is vital to pathological review or medical procedure. Without verification, potential sources of error in pathological labs include specimen mix-up or cross-contamination from “floaters.” Tissue samples taken for pathologic analysis can be inadvertently mislabeled during sample collection or the time of pathology review. Misidentifying patients' samples can have serious consequences for patient management, as well as the treatment plan.

Bio-Synthesis offers Tissue DNA Identification and Testing Services for hospitals that are seeking to eliminate ID errors which compromise a patient’s well-being. Eliminating these errors will also help prevent hospitals from potential legal issues. Our services are performed at our accredited human tissue DNA fingerprinting/Genotyping facilities led by a team of scientists with extensive experience in multiplex STR to confirm tissue DNA identity. Bio-Synthesis also offers customized bioinformatics data analysis to differentiate tested specimens with sample pools specified by our clients.

Our commitment to total quality management (TQM) assures that our customers receive only the most highly discriminating methods for the confirmation of specimen identity and detection of patient sample cross-contamination and misidentification. All of our testing services are in compliance with current good tissue practices (CGTP) to validate your tissue banks.

If you have any questions or for additional information, please contact us or send an email to

When to Conduct Pathological Specimen DNA Identity Testing

  • Determining specimen identity in the case of suspected mislabeling
  • Confirming possible tissue contaminants (“floaters”) identified on histologic block or slide

BSI's Human Tissue Specimen Authentication

Bio-Synthesis offers the most comprehensive STR DNA testing available by examining of a panel of 15 highly polymorphic genetic markers plus one gender marker (amelogenin), to generate an STR DNA profile that uniquely identifies a patient sample. The results are reported as the number of identical or nonidentical alleles between the known and unknown samples. If the suspected source tissue is provided, an interpretation as to the identity of the unknown specimens also is given. If the suspected contaminant and patient’s known sample derived from unrelated individuals, there is a > 99.9999% likelihood that one or more loci will be different.

BSI uses STR (Short Tandem Repeat) Analysis for Clinical Specimen Authentication

DNA fingerprinting analysis by PCR is a highly accurate technique for determining the patient identity of a tissue sample. This assay uses PCR amplification of short tandem repeats (STRs) with number of highly polymorphic unlinked loci. Each STR locus has two alleles, and each allele has a specific length that is stably inherited. PCR amplification of 15 highly polymorphic STR loci [D3S1358, THO1, D21S11, PentaE, D5S818, D13S317, D7S820, D16S539, CSF1PO, PentaD, vWA, D8S1179, TPOX, D18S51, FGA] and a sex chromosome specific locus (amelogenin) is performed. The loci used for patient sample identification are utilized for human sample identity, and are not designed to detect non-human DNA.

Specimen Requirements

DNA STR analysis can be performed on formalin-fixed, paraffin-embedded tissue, blood in EDTA, or fresh/frozen tissue. The submitting pathologist must designate the suspected contaminant/mix-up as well as the patient’s known sample with which to compare. It is optional to include the suspected source tissue.

Testing on whole Blood and Bone Marrow

  1. Samples collected in purple-topped tubes (sodium EDTA anticoagulant) should be shipped on wet ice by FedEx overnight.
  2. 2 ml of blood or bone marrow is needed (Do not freeze whole blood or bone marrow). However, the scientist could extract and isolate DNA and send us genomic DNA. The gDNA should have the concentration 20 ng/ul and the volume 100 ul. The gDNA must be in distilled water and send with regular FedEx.

Testing on Solid Tumor/Frozen Tissue Specimens

  1. Lymph node, skin or other tissue biopsies may be utilized as a source of DNA for molecular assay. Each should be obtained in a sterile manner and transported frozen to preserve DNA integrity.
  2. 10-20 mg of tissue generally is sufficient for DNA STR testing. Again, the scientist could extract and isolate DNA and send us 20 ng/ul and the volume of 100 ul.

Testing on Paraffin-Embedded Tumor Tissues

For DNA testing, we can utilize paraffin-embedded tissue or sections prepared on glass slides. If paraffin material is used for testing, specific requirement will be required.

Slide sample requirement:

  1. Thickness: 6 micron
  2. No cover glass
  3. No dye/stained
  4. No fat tissue
  5. 4-5 slides

How to order tissue DNA testing

  1. To place an order please use the Online Tissue Specimen DNA Identification Order Form.
  2. To submit a sample for analysis, make a printout of the form Tissue Specifimen Identification information and mail to our lab:
    Bio-Synthesis Cell Line Laboratory, 612 E. Main Street, Lewisville, Texas 75057, USA
  3. If you have any questions or would like to inquire about the status of your order, please contact us.
  4. We accept POs and major credit cards . The purchase will be listed on your statement as “Bio-Synthesis, Inc.”