800.227.0627

Modified Oligonucleotides for Colorimetric Detection & Affinity Capture

Bio-Synthesis offers modified oligos or oligonucleotides useful for colorimetric detection and affinity capturing application. Oligonucleotides can be modified using molecular tags such as biotin, dyes or streptavidin. Furthermore, other molecular labels or tags such as digoxigenin, alkaline phosphatase, horseradish peroxidase (HRP), or gold nanoparticles amoung others can be conjugated to oligonucleotides as well.

Biotin is a functional affinity tag often attached to biomolecules such as carbohydrates, peptide, proteins, and oligonucleotides. Since biotin binds specifically to streptavidin and avidin with a very high affinity it is often used in mobility shift assays, or for affinity purification together with streptavidin or avidin beads for column or spin-down chromatography.

Name Modification Postion within oligo
Biotin [Btn] colour1
Biotin dT [Btn dT] colour2
5'-Biotin [5Btn] cell6
Biotin-TEG [BtnTEG] colour4
3'-Biotin [3Btn] colour5
Dual Biotin [Dual Btn] cell3
Horseradish Peroxidase (HRP) [HRP] colour7
Alkaline Phosphatase (AP) [AP] colour8
Gold Nanopariticles colour9
Oligo Magnetic Bead Conjugates colour10

Quality Control:

Analytical HPLC, Gel eletrophoresis, and MALDI-TOF Mass Spectrometry

Delivery times:

5-7 Working days

Packaging:

Dried

Shipping conditions:

Room temperature

Storage conditions:

-20 oC to -70 oC

Oligonucleotides are stable in solution at 4 oC for up to 2 weeks. Properly reconstituted material stored at -20 oC should be stable for at least 6 months. Dried DNA (when kept at -20 oC) in a nuclease-free environment should be stable for years.

Ordering & Contact Information

  • Please contact us for additional information or send an email to info@biosyn.com.
  • You may also request an online quote.
  • To contact us by phone, please Call 972-420-8505 (USA) 800-227-0627 (INTL.) or Fax 972.420.0442
  • Orders may be placed using a purchase order (PO) or by credit card through our secure online ordering system.
  • When using a credit card ( creditcard ) it will be billed under "Bio-Synthesis, Inc."