Digoxigenin Oligo Labeling

Digoxigenin Labeling

Like biotin, digoxigenin (DIG) is mainly used as a non-isotopic label for DNA and oligonucleotides in a wide range of applications. This small hapten can be conjugated to amino-modified oligos with digoxigenin-NHS-ester. The positions of amino-modification can be specified within an oligo. The 5' terminus can be labeled with a C6 or C12 linker. C12 may be preferable. Linker arm length determines accessibility of the digoxigenin label to the detecting antibody. Amino-dT can be substituted for dT at internal positions of the oligo. The spacing between labels should be maintained at 10 or more bases to prevent steric limitations on antibody recognition of the digoxigenin label. Multiple labels are necessary for northern and southern hybridization probes.

Digoxigenin	Desalted Qty.	5'-Mod	Internal	3' -mod
250 nmol	15 OD	$170	NA	$270
1.0 µmol	35 OD	$200	NA	$300
Digoxigenin dT	Desalted Qty.	5'-Mod	Internal	3' -mod
250 nmol	15 OD	$200	$200	NA
1.0 µmol	35 OD	$280	$280	NA

Purification

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD₂₆₀ In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

For additional information, please Contact us.