Bio-Synthesis offers dSpacer abasic oligonucleotide modification at various scales.
dSpacer, also known as abasic furan, is a tetrahydrofuaran derivative containing
a methylene group occupied in the 1 position of 2’-deoxyribose. dSpacer, similar to
C3 spacer, is commonly used to mimic an abasic site in an oligonucleotide.
In DNA, abasic sites are produced by hydrolysis of the glycosidic linkage to the
nucleotide base, leaving the sugar-phosphate backbone at that position. In
the cell, abasic site occurs after a spontaneous depurination/depyrmidination event,
by UV ionizing radiation or as a Base Excision Repair (BER) intermediate. Because such sites are fragile, they are easily susceptible to single-stranded/double-stranded
breakage. If not repaired by the BER mechanism, abasic lesions often lead to
mutation by translesion synthesis during replication. The particular base incorporated
opposite to the lesion varies depending on the organism and environmental conditions.3
dSpacer is used as an abasic site mimic in synthetic oligonucleotides because not only is it
structurally similar to the natural site, but it is also considerably
more stable, and thus can tolerate the chemical conditions used in oligo synthesis
and purification.4 One or more consecutive dSpacer modifications can also be used
to provide varying amounts of separation between different parts of an oligo
sequence.
Contact Bio-Synthesis for dSpacer Abasic Oligonucleotide Modification Service.
Purification:
Spacer modified oligonucleotide can be accomplished by various methods. Desalting
or cartridge purification is acceptable, however, additional purification by HPLC
is strongly recommended.
Price:
A 10% discount for more than two spacer modifications is applied.
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5' dSpacer Abasic Modification
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100 nmole RNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
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$120.00
$120.00
$180.00
$300.00
$600.00
$120.00
$120.00
$180.00
$300.00
$600.00
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Internal dSpacer Modification
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100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
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$120.00
$120.00
$180.00
$300.00
$600.00
$120.00
$120.00
$180.00
$300.00
$600.00
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3' dSpacer Abasic Modification
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100 nmole DNA oligo
250 nmole DNA oligo
1 µmole DNA oligo
5 µmole DNA oligo
10 µmole DNA oligo
100 nmole RNA oligo
250 nmole RNA oligo
1 µmole RNA oligo
5 µmole RNA oligo
10 µmole RNA oligo
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$135.00
$135.00
$200.00
$400.00
$800.00
$135.00
$135.00
$200.00
$400.00
$800.00
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References:
- indahl, T. Instability and decay of the primary structure of DNA. Nature. (1993),
362: 709-715.
- Nilsen, H., Krokan, H.E. Base excision repair in a network of defence and tolerance.Carcinogenesis
(2001), 22: 987-998.
- Lehman, A. Replication of damaged DNA by translesion synthesis in human cells. FEBS
Letters. (2005), 579: 873-876.
- Takeshita, M., Chang, C.N., Johnson, F., Will, S., Grollman, A.P. Oligodeoxynucleotides
containing synthetic abasic sites. Model substrates for DNA polymerases and apurinic/apyrimidinic
endonucleases. J. Biol. Chem. (1987), 262: 10171-10179.