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Fastype™ Molecular Detection Kits for Human Infectious Diseases

Bio-Synthesis Inc. is proud to introduce the Fastype™ Conjunctivitis Adenovirus and Chlamydia DNA PCR-SSP Detection Kits to the molecular infectious diseases testing labs. The molecular test is also known as Nucleic Acid Amplification Test, it is based on the amplification of the present of target DNA in the specimen rather than the conventional method of cell or bacteria culture.

The primary features of the Fastype™ kits are listed as following:

  • All primers, dNTPs and PCR buffer are pre-aliquot and lyophilized in high-quality PCR thermal cycle 0.2ml color coded micro strip tubes. Genomic DNA template of Adenovirus and Chlamydia are also added in the Positive Control Kits respectively for monitoring of PCR condition and the comparison of final amplified fragment.
  • Fastype™ SSP Kits reduce the preparation time to less than 10 minutes.
  • Lyophilized primers, dNTPs and PCR buffer are more stable than in liquid form under ambient condition, and the final lyophilized red dot (reagents) in the bottom of each PCR strip tube can distinguish clearly from the regular PCR strip tubes.
  • The PCR Protocol for all Fastype™ System is kept the same regardless of the kit type.
  • The PCR protocol, Certificate of Product and gel loading dye are also included in the shipment, and we have strong technical support.

Adenoviruses

Adenoviruses are a group of viruses that infect the membrane of the eyes, respiratory tract, the intestines, and urinary tract, they are highly contagious. Pinkeye (Conjunctivitis) always occurs in school children when the virus cause inflammation of the conjunctiva, and they also account for about 10% of acute respiratory infections in children and are a frequent cause of diarrhea. The three testing kits in this category are described as following:

Cat Number Product Name Descriptions
20 – AdTu – FA Fastype™ Adenovirus PCR – SSP Detection Kit A It is a single PCR – SSP reaction kit that detects Adenovirus DNA from infected specimens. This is the first round of the PCR amplification, and the amplified end product is 1,004 bp (base pair). The final lyophilized detection reagents are lyophilized in amber color PCR strip tubes
20 – nAdTu – FB Fastype™ Adenovirus Nested PCR – SSP Detection Kit B It is a single PCR – SSP reaction kit, it is used as a second round PCR to amplify 20 – AdTu – FA amplicon. The final amplified product is 956 bp, and the products are prepared in blue color PCR strip tubes.
20 – AdTu – FC+ Fastype™ Adenovirus PCR – SSP Positive Control Kit It is a single PCR – SSP reaction kit, Adenovirus genomic DNA template is added inside the PCR reaction tube to serve as a positive control and to monitor the PCR condition. The PCR fragment should yield a 1,004 bp band, and the kit is kept in clear PCR strip tubes.

Chlamydia

Chlamydia is a disease caused by the bacteria Chlamydia trachomatis, it is one of the most common sexually transmitted diseases in the U. S., thus, it is often called “the silent epidemic”, because infections are so prevalent yet many people do not know that they are infected. The advantage of molecular DNA – SSP detecting method is that generally more sensitive, specific and speedy than conventional culture,and can therefore identify more positive specimens. The DNA – SSP kits are briefly bdescribed as following:

Cat Number Product Name Descriptions
20 – Ch1/Ch2 – FCh Fastype™ Chlamydia PCR – SSP Detection Kit It is a single PCR – SSP reaction kit, detects omp2 gene Chlamydiaceae Species.The final amplified end product is 587 bp, and all components are lyophilized in the green color PCR strip tube.
20 – Ch1/Ch2 – FChC+, Fastype™ Chlamydia PCR – SSP Positive Control Kit It is a single PCR – SSP reaction kit, Chlamydia genomic DNA template is added inside the PCR – reaction tube to serves as a positive control standard for Chlamydia PCR– SSP detection kit. The final amplified fragment is 587 bp, and the kit is prepared in red color PCR strip tube.

References:

  • Suresh Dalapathy, Therese K. Lily, Sara Roy, and Hajib N. Madhavan.Development and use of nested polymerase chain reaction (PCR) for the Detection of Adenovirus from conjunctivitis specimens. J. Clinical Virology 11:77 – 84, 1998.
  • Hartley, J. C., S. Kaye, S. Stevenson, J. Bennett, and G. Ridgeway.PCR detection and molecular identification of Chlamydiaceae species. J of Clinical Microbiology, 39, 9: 3072 – 3079, 2001.
  • Meijer, A., J. Kwakkel, A. de Vries, L M. Schouls, and J. M.Ossewaarde. Species identification of Chlamydia isolates by analyzing restriction fragment length polymorphism of the 16S – 23S rRNA spacer region. J of Clinical Microbiology, 35, 5:1179 – 1183, 1997.